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pAJM.773

Plasmid

#108527

Purpose

VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

VanRAM + PVanCC-YFP

Expression

Bacterial

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3

Plasmid

#112005

Purpose

For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

Depositor

Lin Tian

Publication

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has service

AAV5 and AAV9

Insert

axon-GCaMP6s-P2A-mRuby3

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-EWB-DIO-cyan pre-eGRASP(p32)

Plasmid

#111589

Purpose

An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Promoter

Ef1a

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-CWB-pre-eGRASP(p32)

Plasmid

#111598

Purpose

An AAV vector that expresses green pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

green pre-eGRASP(p32)

Use

AAV

Promoter

CaMKIIa

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-HaloTag7-SnoopLigase

Plasmid

#105627

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7

Depositor

Mark Howarth

Publication

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

HaloTag7-SnoopLigase

Expression

Bacterial

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV.GFAP.eGFP.WPRE.hGH

Plasmid

#105549

Purpose

AAV expression of EGFP from GFAP promoter

Depositor

James M. Wilson

Has service

AAV5

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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scFv-sfGFP-DNMT3A1

Plasmid

#102278

Purpose

The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

Depositor

Margaret Goodell

Publication

Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

Insert

DNMT3A1 (DNMT3A Human)

Use

Lentiviral

Tags

scFV-sfGFP

Expression

Mammalian

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPB-CAG-rtTA-IRES-Hygro

Plasmid

#102423

Purpose

Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

Depositor

Kian Peng Koh

Publication

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tetracyclin-transactivator (rtTA)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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MCP-YFP

Plasmid

#101160

Purpose

to express MCP-YFP fusion protein

Depositor

Mazhar Adli

Publication

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MCP-YFP

Expression

Mammalian

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Publication

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rock2 (Rock2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCW57.1-DUX4-WT

Plasmid

#99282

Purpose

All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

Depositor

Stephen Tapscott

Publication

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (DUX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDIRECT_23C

Plasmid

#91140

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

Plasmid

#86230

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFC333

Plasmid

#87844

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

Depositor

Uffe Mortensen

Publication

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
ble (bleomycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pT7-VP7SA11

Plasmid

#89167

Purpose

Express Rotavirus SA11 VP7 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rotavirus SA11 VP7

Tags

*see below

Expression

Mammalian

Promoter

T7 promotor

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223_FBXW7_WT

Plasmid

#81795

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_BRAF_p.V600E

Plasmid

#81700

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway Cloning

Mutation

V600E

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pT7-NSP5SA11

Plasmid

#89172

Purpose

Express Rotavirus SA11 NSP5 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rotavirus SA11 NSP5

Expression

Mammalian

Promoter

T7 promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1_miniSOG2 T2A H2B-EGFP

Plasmid

#87410

Purpose

Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

Depositor

Xiaokun Shu

Publication

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mini singlet oxygen generator 2

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRN111

Plasmid

#84463

Purpose

pJB38-NWMN29-30 + SarA_P1-mCherry-Term

Depositor

Reindert Nijland

Publication

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SarA_P1-mCherry from pRN11

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

Plasmid

#85146

Purpose

lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mRuby3-P2A-mTurquoise-MLC

Use

Lentiviral

Tags

mRuby3, mTurquoise

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPaint-TagGFP2-PuroR

Plasmid

#80970

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TagGFP2

Use

Gene tagging

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pTriEx-mCherry-Zdk1

Plasmid

#81057

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Zdk1

Tags

6x His tag, FLAG, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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AgDD

Plasmid

#78289

Purpose

create protein aggregates in living cells

Depositor

Thomas Wandless

Publication

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NES-L106P-sfGFP (FKBP1A Human)

Tags

MLALKLAGLDI

Expression

Mammalian

Promoter

pCAG

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FU- dio PSD95-mCherry- W

Plasmid

#73919

Purpose

Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. Plasmid can also be used to make lentivirus.

Depositor

Elly Nedivi

Publication

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

PSD-95-mCherry

Use

Lentiviral

Tags

mCherry and myc

Expression

Mammalian

Promoter

Ubi-C

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pClneoMyc-LATS1

Plasmid

#66851

Purpose

Express Myc-tagged LATS1 in mammalian cells

Depositor

Yutaka Hata

Publication

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LATS1 (LATS1 Human)

Tags

Myc

Expression

Mammalian

Mutation

P44L mutation in LATS1 compared to GenBank refere…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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paGFP-Omp25

Plasmid

#69598

Purpose

expresses GFP-labeled mitochondrial outer membrane protein 25

Depositor

David Sabatini

Publication

Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

Insert

paGFP-Omp25 (Synj2bp Rat)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

photoactivatable GFP fused to rat Omp25 C-terminus

Promoter

CMV

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCW-GFP-CtIP

Plasmid

#71109

Purpose

mammalian expession of GFP-CtlP

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pLV-tetO-mCherry

Plasmid

#70273

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of mCherry

Depositor

Hubert Schorle

Publication

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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