-
Plasmid#196616PurposePlasmid for integrating tetO7-controlled sfGFP into the HO region in yeastDepositorInsertSuperfolder GFP
UseTagsExpressionYeastMutationPromotertetO7 promoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-mNeonGreen
Plasmid#162034PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsNeon GreenExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IBU-SNAP-Rab7
Plasmid#228141PurposeExpresses SNAP-Rab7 in mammalian cellsDepositorInsertRab7 (Rab7 Mouse)
UseRetroviralTagsSNAPExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd3
Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-JMJD2A
Plasmid#24180PurposeMammalian expression of human JMJD2A with HA tagDepositorInsertJMJD2A (KDM4A Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2
Plasmid#25031DepositorInsertDCP2 (DCP2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-GASC1
Plasmid#24214PurposeMammalian expression of human JMJD2C with HA tagDepositorInsertGASC1 (KDM4C Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-HA-hTet1CD-WPRE-PolyA
Plasmid#39454DepositorInsertHA-hTet1CD (TET1 Human)
UseAAVTagsHAExpressionMammalianMutationaa1418–2136PromoterEF1aAvailable sinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135T
Plasmid#160433PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to ThrDepositorInsertE. coli AlkB
UseTagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Threonine; deleted t…PromoterT7Available sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTMEM-PhoCl-Insert
Plasmid#219674PurposeCaging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarletDepositorInsertTMEM-PhoCl-rtTA3
UseTagsPhoClExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
plx208-CMV-Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit
Plasmid#213538PurposeExpresses the CapC-caged opioid peptide in mammalian cellsDepositorInsertSignal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy Flag GCN5
Plasmid#14106DepositorInsertGCN5 (KAT2A Human)
UseAdenoviralTagsflagExpressionMammalianMutationE189G and K195NPromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLEX301-TagRFP
Plasmid#162035PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsRFPExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28_Human_SAT1_Cterm_His
Plasmid#220487Purposeheterologous expression of SSAT1 for protein purificationDepositorInsertSSAT1 (SAT1 Human)
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Human_SAT1_Nterm_His
Plasmid#220482Purposeheterologous expression of SSAT1 for protein purificationDepositorInsertSSAT1 (SAT1 Human)
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-gRNA-puro
Plasmid#180426PurposeExpresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.DepositorInsertgRNAscr
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3628
Plasmid#191445PurposePrgef-1-Chrimson::UrSL2::wCherry unc-54 3' UTR C.elegans panneural expression of Chrimson RFPDepositorInsertChrimson
UseTagswCherryExpressionWormMutationPromoterPregf-1Available sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pInsert-PhoCl-RER
Plasmid#219676PurposeCaging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2DepositorInsertCD4mScarlet-PhoCl-RER
UseTagsmScarlet and PhoClExpressionMammalianMutationPromoterTREAvailable sinceJune 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH2523
Plasmid#191358PurposePunc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFPDepositorInsertGCaMP3
UseTagsmCherryExpressionWormMutationPromoterPunc-25Available sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-His-hCE
Plasmid#112710PurposeExpresses N-terminally His-tagged human CE/RNGTT in bacterial cellsDepositorInsertRNGTT (RNGTT Human)
UseTags6xHis and Stop codons placed after RNGTT sequence…ExpressionBacterialMutationR594HPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-RFP-IRES-CD
Plasmid#126678PurposeDoxycycline inducible expression of CD along with reporter RFPDepositorInsertRFP-IRES-CD (FCY1 Budding Yeast)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX303-GFP
Plasmid#162032PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH4260
Plasmid#191678PurposePtwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarletDepositorInsertGCaMP6s
UseTagswScarletExpressionWormMutationPromoterPtwk-40Available sinceNov. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLEX304-GFP
Plasmid#162033PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
S12.1_Empty_Bridge
Plasmid#127670PurposeAmpicillin resistant multiplexing backbone without CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH4330
Plasmid#191700PurposePunc-17::GtACR2::wCherry unc-54 3' UTR C.elegans neuron expression of GtACR2 RFPDepositorInsertGtACR2
UseTagswCherryExpressionWormMutationPromoterPunc-17Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4329
Plasmid#191679PurposePrgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural expression of GtACR2 RFPDepositorInsertGtACR2
UseTagswCherryExpressionWormMutationPromoterPregf-1Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
p35S::mCh-HA-P2A-lifeact-mCh
Plasmid#135234Purposeconstitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)DepositorInsertmCh-HA-P2A-lifeact-mCh
UseTagsHA, lifeact, and p2aExpressionPlantMutationPromoter35SAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
S12.2_Bridge + Target
Plasmid#127671PurposeAmpicillin resistant multiplexing backbone with CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Empty Anchor Control
Plasmid#127665PurposeEncodes for puromycin resistance expressed from the EF1a promoterDepositorInsertPuro
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH3666
Plasmid#191447PurposePacr-2s-GCaMP6s::wCherry unc-54 3' UTR C.elegans A/B MN expression of GCaMP6s RFPDepositorInsertGCaMP6s
UseTagswCherryExpressionWormMutationPromoterPacr-2sAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H
Plasmid#146503PurposeInsect Expression of DmDCP1_327-372DepositorInsertDmDCP1_327-372 (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC027.306
Plasmid#171149PurposeBiopterin production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-1_Rluc-Y39x-Fluc
Plasmid#233615PurposeExpression of Rluc-Fluc reporter gene containing 39xYDepositorInsertRluc-Y39x-Fluc
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-4
Plasmid#228986PurposeFor bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-4
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-2
Plasmid#228984PurposeFor bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-2
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-7
Plasmid#228989PurposeFor bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-7
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-38
Plasmid#228979PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-38, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-38
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-1
Plasmid#228983PurposeFor bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-3
Plasmid#228985PurposeFor bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-3
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-44
Plasmid#228981PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-44
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-45
Plasmid#228982PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-45
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-8
Plasmid#228990PurposeFor bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-8
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-5
Plasmid#228987PurposeFor bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-5
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-6
Plasmid#228988PurposeFor bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-6
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-39
Plasmid#228980PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-39
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-TEF2p-LDH-CYC1t
Plasmid#216120PurposeYeast integrative vector for constitutive expression of LDH at the LEU2 locusDepositorInsertLactate dehydrogenase (LDH Bovine)
UseTagsExpressionYeastMutationPromoterTEF2 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-LDH-CYC1t
Plasmid#216121PurposeYeast integrative vector for constitutive expression of LDH at the TRP1 locusDepositorInsertLactate dehydrogenase (LDH Bovine, Bos taurus)
UseTagsExpressionYeastMutationPromoterTDH3 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-GFP-Rab5
Plasmid#228139PurposeExpresses GFP-Rab5 in mammalian cellsDepositorInsertRab5 (Rab5a Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only