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159,371 results

Showing: 10401 - 10440 of 159371 results

  1. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83476-plasmid-map-sequence-id-189195

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  3. NL4-3 mCherry Luciferase

    Plasmid
    #44965
    Purpose
    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
    Depositor
    Warner Greene
    Article
    Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371
    Insert
    mCherry, Luciferase, IRES
    Use
    Lentiviral
    Tags
    Luciferase, IRES and mCherry with D212G
    Expression
    Mutation
    S34C mutation in Nef
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    44965-plasmid-map-sequence-id-352462

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  7. ePPE

    Plasmid
    #183095
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103
    Insert
    Nls-nCas9-NC-nls-MLV-Nls
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A in SpCas9
    Promoter
    Available since
    April 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183095-plasmid-map-sequence-id-358613

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  13. lenti-sgRNA neo

    Plasmid
    #104992
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104992-plasmid-map-sequence-id-213507

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  18. mEos3.2-TOMM20-N-10

    Plasmid
    #57483
    Purpose
    Localization: Mitochondrial Membrane, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pAM5411

    Plasmid
    #132663
    Purpose
    RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance
    Depositor
    Susan Golden
    Article
    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1
    Inserts
    aadA
    YFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PaadA and PconII
    Available since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    132663-plasmid-map-sequence-id-260955

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  23. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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  27. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2 (NES Synthetic)
    Use
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222950-plasmid-map-sequence-id-450583

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  29. KRAB-dSpCas9

    Plasmid
    #205248
    Purpose
    In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    KRAB-dSpCas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205248-plasmid-map-sequence-id-409108

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  33. mPA-GFP-CD81-10

    Plasmid
    #57124
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    CD81 (CD81 Human)
    Use
    Tags
    mPA-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. Mito-SNAP

    Plasmid
    #206480
    Purpose
    Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression
    Depositor
    Erika Holzbaur
    Article
    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7
    Insert
    Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
    Use
    Tags
    SNAPf
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    206480-plasmid-map-sequence-id-435222

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  39. pDONR221-SLC1A4_STOP

    Plasmid
    #161128
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC1A4 (SLC1A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161128-plasmid-map-sequence-id-334669

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Showing: 10401 - 10440 of 159371 results