We narrowed to 27,291 results for: Tat
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Plasmid#76037Purpose3rd generation lentiviral gRNA plasmid targeting human ANKK1DepositorInsertANKK1 (ANKK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK3 gRNA (BRDN0001145644)
Plasmid#75976Purpose3rd generation lentiviral gRNA plasmid targeting human STK3DepositorInsertSTK3 (STK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMPK2 gRNA (BRDN0001145007)
Plasmid#75805Purpose3rd generation lentiviral gRNA plasmid targeting human CMPK2DepositorInsertCMPK2 (CMPK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PIK3R4 gRNA (BRDN0001148885)
Plasmid#75594Purpose3rd generation lentiviral gRNA plasmid targeting human PIK3R4DepositorInsertPIK3R4 (PIK3R4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PIK3R4 gRNA (BRDN0001148908)
Plasmid#75595Purpose3rd generation lentiviral gRNA plasmid targeting human PIK3R4DepositorInsertPIK3R4 (PIK3R4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-L156P
Plasmid#69918PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationL156PPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-MIIa
Plasmid#58684PurposeExpresses LGP2 with a mutation to motif IIa in mammalian cellsDepositorInsertLGP2-MIIa (DHX58 Human)
UseTags3x FLAGExpressionMammalianMutationmutation to Motif IIA, K138E and Y142FPromoterCMVAvailable sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt344B
Plasmid#34602DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-344 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt206A
Plasmid#34599DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-206 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro/RAF1-P207S
Plasmid#131726PurposeMammalian Expression of RAF1DepositorInsertRAF1 (RAF1 Human)
UseLentiviralTagsExpressionMammalianMutationP207SPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TRAP1_WT_V5
Plasmid#82994PurposeGateway Donor vector containing TRAP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertTRAP1 (TRAP1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mCherry-EGFP-LC3B
Plasmid#170446PurposeLentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.DepositorInsertmCherry-GFP-LC3 (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT/CAGGS-NRASG12V-IRES-mVenus
Plasmid#236076PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).DepositorInsertsUseSleeping beauty transposon plasmidTagsExpressionMutationG12VPromoterCAGGSAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT/CAGGS-NRASG12V/D38A-IRES-mVenus
Plasmid#236077PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).DepositorInsertsUseSleeping beauty transposon plasmidTagsExpressionMutationG12V D38APromoterCAGGSAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMIH-TFAM-GFP
Plasmid#113704PurposeFluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selectionDepositorInsertTFAM (Tfam Mouse)
UseRetroviralTagseGFPExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-ICUE
Plasmid#181845PurposeGenetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-ICUE (RAPGEF3 Human)
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-8:TOMM20-mEGFP
Plasmid#87423PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20DepositorInsertTOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
TRIPZ-bioGEF-UBnc-PURO
Plasmid#208044PurposeEnables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycinDepositorInsertUBnc (UBC Human)
UseLentiviralTagsLow-affinity AvitagExpressionMutationL73P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-CMV-PEmax-IRES-ZeoR
Plasmid#207353PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.DepositorInsertPrime editor max (PEmax) enzyme
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pET21b(+)-Is-PETase-W159H-S238F
Plasmid#112203PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationW159H, S238FPromoterN/AAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Chronos-tdTomato]
Plasmid#84484PurposeAAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3 N-MYC TIP60
Plasmid#213003Purposeexpresses human TIP60DepositorInsertKAT5 (KAT5 Human)
UseTagsMYCExpressionMammalianMutationPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dF-HA-KORD-IRES-mCitrine
Plasmid#65417PurposeAAV packaging vector for Cre-dependent, hSyn driven KOR DREADD expression.DepositorUseAAVTagsHA and mCitrineExpressionMutationPromoterhSynAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PE2-IRES-ZeoR
Plasmid#207352PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.DepositorInsertPrime editor 2 (PE2) enzyme
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1Δ499-546-HA-Puro
Plasmid#193768PurposeDoxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1Δ499-546 (CDT1 Human)
UseLentiviralTagsHAExpressionMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ZBTB24_WT_V5
Plasmid#83004PurposeGateway Donor vector containing ZBTB24, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertZBTB24 (ZBTB24 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_5SA-PolyA
Plasmid#112285PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)DepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with 5 Serines (61, 109, 127, 1…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)
Plasmid#208289PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA; mutations in iSpyMacPromoterCMV and T7Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TP53_WT
Plasmid#82754PurposeGateway Donor vector containing TP53, part of the Target Accelerator Plasmid Collection.DepositorInsertTP53 (TP53 Human)
UseGateway entry vectorTagsExpressionMutationP72RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-ChrimsonR-tdTomato
Plasmid#99231PurposeAAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterCaMKIIAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MDM2_WT
Plasmid#82897PurposeGateway Donor vector containing MDM2, part of the Target Accelerator Plasmid Collection.DepositorInsertMDM2 (MDM2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.G469V
Plasmid#82833PurposeGateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationG469VPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-TDP-43 M337V
Plasmid#162609PurposeAllows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationM337VPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PIK3CA_p.H1047R
Plasmid#82824PurposeGateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.DepositorInsertPIK3CA (PIK3CA Human)
UseGateway entry vectorTagsExpressionMutationH1047RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KEAP1_p.R470S
Plasmid#82781PurposeGateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.DepositorInsertKEAP1 (KEAP1 Human)
UseGateway entry vectorTagsExpressionMutationR470S; C624SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7NT*-Bri2 113-231 R221E
Plasmid#138134PurposeExpresseds human Bri2 BRICHOS R221E mutant in E. coliDepositorInserthuman Bri2 BRICHOS R221E (ITM2B Human)
UseTagsNT* tag derived from spider silk proteinsExpressionBacterialMutationPromoterT7Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
UseTags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Chronos-tdTomato
Plasmid#62726PurposeAAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.DepositorHas ServiceAAV8InsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only