We narrowed to 22,837 results for: Sis

1. pX458_miR-290-295KO-gRNA1

   Plasmid

   #172709

   Purpose

   Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   miR-290-295
   

   Use

   CRISPR

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458_miR-290-295KO-gRNA2

   Plasmid

   #172710

   Purpose

   Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   miR-290-295
   

   Use

   CRISPR

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

   Plasmid

   #84258

   Purpose

   Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   Sp sgTRE3G
   PYL1-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   IRES-mCherry and PYL1

   Expression

   Mammalian

   Promoter

   CMV and mouse U6

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1P shABCB7_2

   Plasmid

   #102969

   Purpose

   Suppression of ABCB7

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shABCB7_2 (ABCB7 Human)
   

   Use

   Lentiviral and RNAi

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1P shABCB7_1

   Plasmid

   #102968

   Purpose

   Suppression of ABCB7

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shABCB7_1 (ABCB7 Human)
   

   Use

   Lentiviral and RNAi

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2-cryR;tmem88aCE1-P1Egfp

   Plasmid

   #90148

   Purpose

   contains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   tmem88aE1P1Egfp (tmem88a Zebrafish)
   

   Tags

   EGFP

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pV1464

   Plasmid

   #111440

   Purpose

   N. castellii Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAYC

   Plasmid

   #62657

   Purpose

   Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   iCre
   

   Expression

   Mammalian

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-myc-ASPM CTR

   Plasmid

   #27669

   Depositor

   Joseph Loturco

   Article

   Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

   Insert

   Abnormal spindle-like microcephaly associated (Aspm Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Contains carboxy terminal region of ASPM (amino a…

   Available Since

   March 21, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pDN-D2irTN2AG5kwh

    Plasmid

    #128253

    Purpose

    Mammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    Beta Globin Intron::hTetR::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    CMV D2ir promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. UKNP4.2.1

    Plasmid

    #98267

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. UKNP2.3.1

    Plasmid

    #98210

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBABE-Hygro HoxB9

    Plasmid

    #27022

    Depositor

    Joan Massague

    Article

    Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

    Insert

    HoxB9 (HOXB9 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3.1-hMcl-1(T163A)

    Plasmid

    #25376

    Depositor

    Roger Davis

    Article

    Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

    Insert

    hMCL1 (MCL1 Human)
    

    Tags

    V5 and His tag

    Expression

    Mammalian

    Mutation

    changed threonine 163 to alanine

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

15. R26-EGFP MMEJ donor vector

    Plasmid

    #137926

    Purpose

    R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with micro-homology arms
    

    Use

    Mouse Targeting

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

    Plasmid

    #115490

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMtb CblA

    Plasmid

    #138298

    Purpose

    Expression of Mtb CblA

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Insert

    Mtb CblA
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMtb ATR

    Plasmid

    #138297

    Purpose

    Expression of Mtb ATR

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Insert

    Corrinoid adenosyl transferase
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA6-LRRC38-V5-His

    Plasmid

    #127820

    Purpose

    Expression of human LRRC38 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC38 (LRRC38 Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJMP1290

    Plasmid

    #119267

    Purpose

    construction intermediate

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHygroR-GMRWhite

    Plasmid

    #165886

    Purpose

    Hygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX6p-Meis1a

    Plasmid

    #78144

    Purpose

    bacterial expression of GST-Meis1a

    Depositor

    Francesco Blasi

    Article

    Dardaei et al Proc Natl Acad Sci U S A. 2014 Mar 11;11

    Insert

    Meis1a (Meis1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

    Plasmid

    #127357

    Purpose

    gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    R118G

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. cycLuc_SuMMVs

    Plasmid

    #119210

    Purpose

    Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with SuMMVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPSP6

    Plasmid

    #48150

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJMP1067

    Plasmid

    #119243

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103_VAV3-1/2

    Plasmid

    #91528

    Purpose

    Protein expression and purification of human SH3 domain construct VAV3-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV3-1/2 (VAV3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_SORBS3-1/3

    Plasmid

    #91534

    Purpose

    Protein expression and purification of human SH3 domain construct SORBS3-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SORBS3-1/3 (SORBS3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_VAV3-2/2

    Plasmid

    #91524

    Purpose

    Protein expression and purification of human SH3 domain construct VAV3-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV3-2/2 (VAV3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103_SH3YL1-1/1

    Plasmid

    #91485

    Purpose

    Protein expression and purification of human SH3 domain construct SH3YL1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3YL1-1/1 (SH3YL1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHH0103_NEBL-1/1

    Plasmid

    #91383

    Purpose

    Protein expression and purification of human SH3 domain construct NEBL-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NEBL-1/1 (NEBL Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHH0103_NPHP1-1/1

    Plasmid

    #91358

    Purpose

    Protein expression and purification of human SH3 domain construct NPHP1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NPHP1-1/1 (NPHP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHH0103_PACSIN1-1/1

    Plasmid

    #91372

    Purpose

    Protein expression and purification of human SH3 domain construct PACSIN1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PACSIN1-1/1 (PACSIN1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHH0103_SH3D21-1/3

    Plasmid

    #91296

    Purpose

    Protein expression and purification of human SH3 domain construct SH3D21-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3D21-1/3 (SH3D21 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH0103_MYO15A-2/2

    Plasmid

    #91291

    Purpose

    Protein expression and purification of human SH3 domain construct MYO15A-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MYO15A-2/2 (MYO15A Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDGE671

    Plasmid

    #153237

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    GFP

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p3E-Dusp6DM_S159/197A

    Plasmid

    #67684

    Purpose

    3' entry clone with activated Dusp6 (S159/167A)

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    dusp6 (dusp6 Zebrafish)
    

    Mutation

    S159A, S197A

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGWB527

    Plasmid

    #74869

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBABE-hygro NM23 H1 WT

    Plasmid

    #11364

    Depositor

    Ronald Kahn

    Article

    Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

    Insert

    NM23 H1 (NME1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

40. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTR

    Plasmid

    #67932

    Purpose

    Translational reporter - Lox 5'UTR

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    sfGFP-NLS-P2A-NLS-mCherry (LOX Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge