We narrowed to 23,940 results for: nes

1. enAsCas12a-mSA

   Plasmid

   #182954

   Purpose

   Express enAsCas12a-mSA

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   enAsCas12a-mSA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUAS-hActin-luc2

   Plasmid

   #48081

   Purpose

   Luciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter

   Depositor

   Huda Zoghbi

   Article

   Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   removed hsp70 promoter, inserted amplified 615 bp…

   Promoter

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

3. tol2-mpx-lyn-GFP

   Plasmid

   #35037

   Depositor

   Anna Huttenlocher

   Article

   Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

   Insert

   Lyn-GFP (lyn Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pSicoR human Dicer3

   Plasmid

   #14765

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 Apr 1. ():.

   Insert

   Dicer shRNA (DICER1 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pLV-H1-SGIPZ_NF1 sh1miR

   Plasmid

   #119206

   Purpose

   knockdown of NF1

   Depositor

   Helen Morrison

   Article

   Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

   Insert

   sh1miR targeting NF1 (NF1 Rat, Human, Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCherry_eDHFR_SIM#1

   Plasmid

   #164646

   Purpose

   tag SIM with mCherry and eDHFR

   Depositor

   Michael Lampson, Huaiying Zhang

   Article

   Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

   Insert

   SIM
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-nDsRedFL-miRNT

   Plasmid

   #177808

   Purpose

   A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-NT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1-scrCdh2.1 GFP

   Plasmid

   #209100

   Purpose

   GFP expressing scramble of shRNA targeting Cdh2

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   scrCdh2.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. px330 RBP1 gRNA

   Plasmid

   #165593

   Purpose

   Insertion of RBP1 degron

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   RBP1 (Polr2a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBT344_pattB-pCA-GFP-pA-FRT5

    Plasmid

    #52550

    Purpose

    for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 141_pT3TS_iCre

    Plasmid

    #171793

    Purpose

    Plasmid for optimised iCre RNA synthesis using T3 promoter.

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Insert

    optimised Cre for RNA expression
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pME-nlsGal80(opt)

    Plasmid

    #51618

    Purpose

    vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80(opt) (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Tags

    NLS

    Expression

    Mutation

    codon optimized

    Promoter

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Col1A-5'probe

    Plasmid

    #20732

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    Col1A-5'probe (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Sox1/2/3-2373/+3>nls::Cas9::nls

    Plasmid

    #59989

    Purpose

    Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Sox1/2/3 (SoxB1) -2373/+3

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHSP70B-CP6-TK

    Plasmid

    #165881

    Purpose

    Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid

    #117070

    Purpose

    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

    Depositor

    Kim Failor

    Inserts

    Cas9
    SacB
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOD_A0908

    Plasmid

    #91027

    Purpose

    Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TREX2-P2A-AtCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EC1.2

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)-PM-CaNARi

    Plasmid

    #64850

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    Lyn-CaNARi
    

    Use

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

    Plasmid

    #21800

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD14 (Psmd14 Mouse)
    

    Use

    Tags

    GST and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-Merlin-Flag-HA

    Plasmid

    #27103

    Depositor

    Filippo Giancotti

    Article

    Li et al Cell. 2010 Feb 19. 140(4):477-90.

    Insert

    Merlin (Nf2 Mouse)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pENTR-Arl13b1

    Plasmid

    #40870

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

22. SB52

    Plasmid

    #21487

    Depositor

    Shoumo Bhattacharya

    Article

    Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2009

    Availability

    Academic Institutions and Nonprofits only

23. pCRISPR_eGFP_314

    Plasmid

    #111822

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 294-314
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSuper mouse DGCR8-2

    Plasmid

    #14773

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    DGCR8 shRNA (Dgcr8 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pRK5-mFzd6-1D4

    Plasmid

    #42268

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 6 (Fzd6 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pVenus-Sec16A

    Plasmid

    #36157

    Purpose

    Expresses Sec16A as a Venus FP fusion

    Depositor

    David Stephens

    Article

    Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

    Insert

    Sec16A (SEC16A Human)
    

    Use

    Tags

    Venus (EYFP derivative)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pIS1 Cyclin D2 long UTR

    Plasmid

    #21645

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 long UTR (CCND2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA-LEU2-I-SceI

    Plasmid

    #67595

    Purpose

    I-SceI expression regulated by tetracycline inducible system in S. pombe

    Depositor

    Kurt Runge

    Article

    Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

    Inserts

    nmt terminator
    I-SceI
    TetR
    

    Use

    Gene targeting in yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti_CMV:Flag-CeNLuc_SV40:PuroR

    Plasmid

    #183036

    Purpose

    Expresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    CeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. lentiCRISPR - DDX3Y sgRNA 2

    Plasmid

    #70657

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pRK5-mFzd10-1D4

    Plasmid

    #42272

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 10 (Fzd10 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pSicoR human Drosha3

    Plasmid

    #14768

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    Drosha shRNA (DROSHA Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

34. PA-RL-mCherry

    Plasmid

    #78861

    Purpose

    DULIP negative control (bait) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMX-PGC1

    Plasmid

    #6

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Cell 1998 Mar 20;92(6):829-39.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2005

    Availability

    Academic Institutions and Nonprofits only

36. pGL3-basic-Polr2a promoter_600bps

    Plasmid

    #21111

    Depositor

    Dean Tantin

    Article

    Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

    Insert

    Polr2a promoter_600bps (POLR2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

37. mCherry-eDHFR-SIMvada

    Plasmid

    #164649

    Purpose

    SIM mutant tagged with mCherry and eDHFR

    Depositor

    Michael Lampson, Huaiying Zhang

    Article

    Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

    Insert

    SIMvada
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSicoR mouse Dicer3

    Plasmid

    #14781

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    Dicer shRNA (Dicer1 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

39. pME-puro-CLIP-FLAG-CD59

    Plasmid

    #50375

    Purpose

    Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    CLIP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pB194

    Plasmid

    #61154

    Purpose

    Expression of KIN28 under GAL1 promoter in yeast

    Depositor

    Charles Brenner

    Article

    Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

    Insert

    TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only