We narrowed to 6,273 results for: nls
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Plasmid#185799Purposepcp-12xSunTag component for SunRISER SRv1.2DepositorInsertpcp-12xSunTag
TagsFactorXa site, HA, and NLSExpressionMammalianPromoterhuman ubiquitin C promoterAvailable SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NLS-QPAS1-VP16
Plasmid#186187PurposeAAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activationDepositorInsertNLS-QPAS1-VP16
UseAAVPromoterCAGAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH321-1-Tier1-PhCMV-dCas9-3xNLS
Plasmid#169595PurposeTier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).DepositorInsertdead S.pyogenes Cas9
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKSB- PUb-NLSmTurqSV40 GGGG AAGA
Plasmid#174535PurposePUb-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsertPUb-mTurquoise BsaI cassette
ExpressionInsectPromoterAedes aegypti PolyubiquitinAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
Plasmid#172208PurposeAAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL
Plasmid#172205PurposeAAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-dt/CBA-EGFP-NLS
Plasmid#163918PurposeContains bidirectional reporter genes dtomato and nuclear-targeting EGFP.DepositorInsertdtomato and EGFP
UseLentiviralExpressionMammalianMutationAdded nuclear localization signal to EGFPAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCaggs-NLS-PAmCherry1-GSS-EGFP
Plasmid#158000PurposeExpression of nucleus localized PAmCherry1 and EGFP fusion proteinDepositorInsertNLS-PAmCherry1-GSS-EGFP
ExpressionMammalianPromoterActin promoter with CMV enhancerAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise-3XNLS-WPRE
Plasmid#159949Purposeoptical regulation of nuclear exportDepositorInsertmTurquoise-3XNLS
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-Ypet-3XNLS-WPRE
Plasmid#159950Purposenucleus markerDepositorInsertYpet-3XNLS
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(+7A)-IDR-YFP-NLS
Plasmid#145278PurposeMammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLSDepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-037 NLSNoLS(D-G)//Esp3I
Plasmid#130239PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS
UseSynthetic BiologyAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E7-dTom-nls-dTom
Plasmid#135642PurposeAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E7 regulatory elementDepositorInsertdTom-nlsdTom
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E9-dTom-nls-dTom
Plasmid#135644PurposeAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E9 regulatory elementDepositorInsertdTom-nlsdTom
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)
Plasmid#133795PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a-3NLS-Klf4 TALE-GCN4
Plasmid#120546PurposeExpress 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human KLF4 geneDepositorAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
ELK1 A box-NLS pcDNA3.1/HisC
Plasmid#121096PurposeExpression of the A box fragment of ELK1DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
ELK1 B box-NLS pcDNA3.1/HisC
Plasmid#121095PurposeExpression of the B box fragment of ELK1DepositorAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS
Plasmid#107303PurposeExpresses ZFP-VEGFA-TS2 fused to FRB in mammalian cellsDepositorInsertZFP_VEGFA-TS2
UseCRISPRTags2xNES, 3x Flag, 2xNLS, and FRBExpressionMammalianPromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS
Plasmid#107304PurposeExpresses ZFP-VEGFA-TS3 fused to FRB in mammalian cellsDepositorInsertZFP_VEGFA-TS3
UseCRISPRTags2xNES, 3x Flag, 2xNLS, and FRBExpressionMammalianPromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta2
Plasmid#100871PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
Tags3xFLAGExpressionMammalianMutationtruncated protein 71-246aaPromoterCMVAvailable SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-Flag-NLS-hSPDEF delta3
Plasmid#100876PurposeLentiviral expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationtruncated protein 243-335aaPromoterTRE2Available SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta3
Plasmid#100872PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
Tags3xFLAGExpressionMammalianMutationtruncated protein 244-335aaPromoterCMVAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta1
Plasmid#100870PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
Tags3xFLAGExpressionMammalianMutationtruncated protein 1-248aaPromoterCMVAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
Plasmid#68276PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLSDepositorInsertSte7DS NLS(4E) mCherry
TagsmCherryExpressionYeastPromoterRPS2Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-DBD-NLS
Plasmid#51841PurposeFlag-CHES1L mutant encoding the DNA binding and NLS domains onlyDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlag and NLSMutationcontains CHES1 amino acids 110-205Available SinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
SPL11p::pCGTAG (SPL11p::NLS-GFP)
Plasmid#27411DepositorInsertSPL11 promoter (AT1G27360 Mustard Weed)
MutationSPL11 promoter transcriptionally fused to a nucle…Available SinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)
Plasmid#115141PurposeCMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLSDepositorInserthuman codon optimized Orf2mor anti-CRISPR protein
TagsNLS(SV40)ExpressionMammalianAvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1a-dCas9-HA-BFP-KRAB-NLS
Plasmid#102244PurposeLentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoterDepositorInsertdCas9-HA-BFP-KRAB-NLS
UseCRISPR and LentiviralTagsHA-TagBFP-KRAB-NLSExpressionMammalianPromoterEF1aAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)
Plasmid#140534PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2DepositorInsertOsTIR1(F74G) mAID-EGFP-NLS TOL2
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-KanMX6
Plasmid#228022PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-HIS3MX6
Plasmid#228023PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-HISMX6
Plasmid#228027PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with HISMX6 markerDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-KanMX6
Plasmid#228026PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-1: ORF5-TBP6.7-Pin domain-NLS
Plasmid#132543PurposeExpresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cellsDepositorInsertCIRTS-1: ORF5-TBP6.7-Pin domain-NLS
TagsFLAG tagExpressionMammalianPromotercmv d0Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87828PurposeHigh-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
TagsEGFP and mCherryExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
ExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Per2)-DIO-intron2-Venus-NLS-D2
Plasmid#110057PurposePer2 transcription fluorescence reporterDepositorInsertPer2 promoter and Venus (Per2 Mouse)
UseAAV and Cre/LoxTagsNLS-D2ExpressionMammalianPromoterPer2Available SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-pSV40(4)-pmRevErbA-mScaI3-NLS-Pest
Plasmid#240118PurposeA lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.DepositorInsertmurine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded SV40-Enhancer+OriAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)
Plasmid#204619PurposeExpression vector of Cascade and Cas3. Puromycin selectable.DepositorInsertCas7, Cas5, Cas8, Cas11, Cas6, and Cas3
UseCRISPRTagsEach protein has C- and N-terminal SV40 NLSPromoterEF1aAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc
Plasmid#113899PurposeMammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminaseDepositorInsertTetR-HA-NLS-P2A-BSD-Myc
UseLentiviralTagsHA-MycPromoterCMV-MIEAvailable SinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140220PurposeConstitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activatorDepositorInsertdLbCpf1
UseCRISPRTagsminiVPR-mCherryExpressionMammalianMutationD832APromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Neo-PIP-NLS-mVenus
Plasmid#118619PurposeLentiviral expression of the cell cycle marker PIP-NLS-mVenusDepositorInsertPIP-NLS-mVenus
UseLentiviralTagsmVenusExpressionMammalianPromoterhPGKAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS-FLAG-cGAS
Plasmid#127659PurposeOver-express EGFP-NLS-FLAG-cGASDepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-GFP-PGK-Puro
Plasmid#110869PurposeLentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato
Plasmid#51085PurposeNeuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV Retrograde and AAV1InsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianPromoterhuman Synapsin1Available SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianPromoterEF1aAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)
Plasmid#225754PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA
Plasmid#118617PurposeLentiviral expression of mTurq2 tagged PCNADepositorInsertTK-NLS-mTurq2-PCNA (PCNA Human)
UseLentiviralTagsmTurq2ExpressionMammalianPromoterhPGKAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only