164,223 results

1. pLKO.1 puro_shDDX58_230212

   Plasmid

   #167293

   Purpose

   Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette.

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   shRNA hsDDX58 (RIGI Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV4-3 HA/IkB-alpha

   Plasmid

   #21985

   Depositor

   Warner Greene

   Article

   Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

   Insert

   I-kappa B alpha (NFKBIA Human)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY49

   Plasmid

   #128915

   Purpose

   mammalian expression, proximity labelling, mitochondrial relocalisation

   Depositor

   Sean Munro

   Article

   Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

   Insert

   Rab11BSN-BirA*-HA-MAO (RAB11B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223-ACVR1C

   Plasmid

   #23615

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ACVR1C (ACVR1C Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

5. CSII-PPM1H-mApple

   Plasmid

   #198473

   Purpose

   Mammalian expression of PPM1H-mApple

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   PPM1H-mApple (PPM1H Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Flag-IDH1-R132H

   Plasmid

   #62907

   Purpose

   Expresses R132 mutant IDH1 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

   Insert

   IDH1 (IDH1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deleted Arg132 (R132)

   Promoter

   Available Since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-flag-myr-Akt1-IRES-GFP

   Plasmid

   #65063

   Purpose

   mammalian retroviral expression plasmid

   Depositor

   Kira Gritsman, Jean Zhao

   Article

   Kharas et al Blood. 2010 Feb 18;115(7):1406-15. doi:

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   flag and myristoylation tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pPagP

   Plasmid

   #112125

   Purpose

   Bacterial expression of outer membrane protein PagP, wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   PagP (pagP E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Plasmid

   #218667

   Purpose

   pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   mitoLbNOX
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG and MTS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC5A4

    Plasmid

    #132170

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC5A4 (SLC5A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pJEP217-Lenti-CMV-GFP-WPRE-pA

    Plasmid

    #62925

    Purpose

    pLenti vector backbone designed to express GFP from a CMV promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    1.3αCaMKII and CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Beta-2-adrenergic receptor-YFP

    Plasmid

    #67146

    Purpose

    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Beta-2-adrenergic receptor-YFP (ADRB2 Human, Aequorea victoria)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF806-reci

    Plasmid

    #186711

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Cloning recipient (reci).

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-EGFP-C1-HsEDC4_S

    Plasmid

    #147455

    Purpose

    Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsEDC4 (EDC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJS61

    Plasmid

    #207585

    Purpose

    GFP-TolA expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TolA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. tdTomato-ER-5

    Plasmid

    #58098

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    ER (CALR Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

17. L-CRISPR-CTN (MLL-ENL)

    Plasmid

    #69212

    Purpose

    Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. F5-bio-His

    Plasmid

    #53426

    Purpose

    Expresses full-length Coagulation factor V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    F5 (F5 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBtet-Bla

    Plasmid

    #60510

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTR-345 pUC19-tNGFR-P2A-IL2RB N

    Plasmid

    #186064

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL2RB (IL2RB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pN40

    Plasmid

    #128898

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab2AQL-BirA*-HA-MAO (RAB2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HA-NEDD8

    Plasmid

    #18711

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

    Insert

    NEDD8 (NEDD8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AnkyrinB-2XHA

    Plasmid

    #31057

    Depositor

    Vann Bennett

    Article

    Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

    Insert

    Ankyrin B (ANK2 Human)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CMPE-ePPEplus

    Plasmid

    #205242

    Purpose

    For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV Tmx1-V5

    Plasmid

    #175138

    Purpose

    Lentiviral expression of V5-tagged mouse Tmx1

    Depositor

    Larry Gerace

    Insert

    Tmx1 (Tmx1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFastBac Dual wt p110α/p85α

    Plasmid

    #199302

    Purpose

    co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

    Depositor

    Peter Vogt

    Article

    Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

    Insert

    His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
    

    Use

    Tags

    Avi and His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AICSDP-116:PCNA-mEGFP

    Plasmid

    #170818

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA

    Depositor

    Allen Institute for Cell Science

    Insert

    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    June 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pPLV_C1

    Plasmid

    #186415

    Purpose

    Endogenous murine RNase inhibitor production for cell-free lysate

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

    Insert

    Murine RNase Inhibitor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 2xmyc-RAB10

    Plasmid

    #164631

    Purpose

    Expresses RAB10 in mammalian cells with a 2xmyc tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    Ras-related protein Rab-10 (RAB10 Human)
    

    Use

    Tags

    2xmyc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA 3.1 myc-Rab10

    Plasmid

    #208366

    Purpose

    Mammalian expression of myc-tagged human Rab10

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    myc-Rab10 (RAB10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available Since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTK_Msx1_enh_264_Citrine

    Plasmid

    #130610

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Msx1_enh_264
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3 2xFLAG-2xSTREP E2F4

    Plasmid

    #236439

    Purpose

    transient overexpression of E2F4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F4 (E2F4 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1(+)Gα15-244-NLuc

    Plasmid

    #207154

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNA15 (GNA15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position E(244)N

    Promoter

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

    Plasmid

    #117661

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-alphaf(I)-E2-Crimson
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-RAB-EKARev

    Plasmid

    #118474

    Purpose

    ddRFP-based single-color biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LZRS-Mek1-wt1 (wt1)

    Plasmid

    #21196

    Depositor

    Paul Khavari

    Insert

    Mek1 (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

37. pScI_dCas9-CDA_J23119-sgRNA

    Plasmid

    #108550

    Purpose

    Bacterial Target-AID vector with sgRNA

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Inserts

    dCas9-PmCDA1
    Streptococcus pyogenes sgRNA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    J23119 and lambda OR

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HRE-doUnaG

    Plasmid

    #201713

    Purpose

    Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHT-trxA.ads

    Plasmid

    #47383

    Purpose

    Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Insert

    ads
    

    Use

    Tags

    6xHis and trxA

    Expression

    Bacterial

    Mutation

    Promoter

    Pgrac

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. JB40

    Plasmid

    #128901

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab5ASN-BirA*-HA-MAO (RAB5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge