159,557 results

1. pAAV.CAG.LSL.EGFP

   Plasmid

   #100047

   Purpose

   AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5FRT/TO-Npl4-Strep-HA

   Plasmid

   #113495

   Purpose

   Expression of human Npl4 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Npl4 (NPLOC4 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PS-CFP2-Clathrin-15

   Plasmid

   #57220

   Purpose

   Localization: Clathrin Vesicles, Excitation: 400 / 490, Emission: 468 / 511

   Depositor

   Michael Davidson

   Insert

   Clathrin (CLTB Human)
   

   Use

   Tags

   PS-CFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

   Plasmid

   #90332

   Purpose

   CRISPR interference

   Depositor

   Dirk Heckl

   Article

   Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

   Inserts

   dCas9
   na
   SFFV
   P2A-BSD
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. V5-LOV-Turbo1-G3BP1_pTRE-G418

   Plasmid

   #202015

   Purpose

   expresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-LOV-Turbo1-G3BP1
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-Tight

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL01009::AtU6p

   Plasmid

   #46968

   Purpose

   Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RMCE wild type DNA-PKcs

   Plasmid

   #233911

   Purpose

   Low copy number provides expression of human DNA-PKcs

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   dna-pkcs cDNA (PRKDC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

   Plasmid

   #38262

   Depositor

   Robert Lefkowitz

   Article

   Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

   Insert

   β-arrestin2 (Arrb2 Rat)
   

   Use

   Tags

   Flag and PIP5K Iα core kinase domain (residues 18…

   Expression

   Mammalian

   Mutation

   Arg 233, Arg237, and Lys251 converted to Glutamin…

   Promoter

   CMV

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

9. Str-KDEL_neomycin

   Plasmid

   #65306

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
   

   Use

   Tags

   KDEL motif and Signal Sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-C1-mTrim21ΔPRYSPRY

    Plasmid

    #105518

    Purpose

    Expression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cells

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of PRYSPRY domain (aa1-281)

    Promoter

    CMV

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-Desmoglein2-C-18

    Plasmid

    #55031

    Purpose

    Localization: Desmosomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Desmoglein2 (DSG2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-PEmax-SpG

    Plasmid

    #211549

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBad-Xis/Int Reset Flipper

    Plasmid

    #38207

    Depositor

    Drew Endy

    Article

    Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

    Inserts

    Bxb1-Excisionase
    Bxb1 Integrase
    

    Use

    Synthetic Biology; Biobrick cloning vector

    Tags

    Expression

    Mutation

    Promoter

    pBAD

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid

    #196727

    Purpose

    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    TRE

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTECH-MmAcK3RS(IPYE)

    Plasmid

    #104071

    Purpose

    Expression plasmid encoding MmAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp MmAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-AN-HA_WT RAD23A

    Plasmid

    #201458

    Purpose

    Expresses human RAD23A with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUGW-VGLUT1-pHluorin

    Plasmid

    #207470

    Purpose

    Lentiviral expression of VGLUT1-pHluorin

    Depositor

    Robert Edwards

    Insert

    VGLUT1 (Slc17a7 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEVY-GL

    Plasmid

    #51225

    Purpose

    bi-directional expression vector for yeast, galactose inducible, LEU2 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1,10

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tdEos-CDC42-C-10

    Plasmid

    #57596

    Purpose

    Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Insert

    CDC42 (CDC42 Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pHH0103_YWHAG_14-3-3

    Plasmid

    #109905

    Purpose

    Protein expression and purification of YWHAG_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAG_14-3-3 (YWHAG Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPUR-hU6-sgRNA-Sirius-8XMS2

    Plasmid

    #121942

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEB2-mScarlet

    Plasmid

    #104006

    Purpose

    Plasmid encoding mScarlet

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mScarlet
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pORTMAGE312B

    Plasmid

    #128969

    Purpose

    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    PapRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    xylS-Pm

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ET8-GPIba-high-6His

    Plasmid

    #180251

    Purpose

    High affinity mutant of human platelet membrane protein GP1b alpha

    Depositor

    Timothy Springer

    Article

    Jiang et al J Mol Biol. 2019 Mar 29;431(7):1380-1396

    Insert

    human GPIbalpha 1-290aa with mutation G233V and M239V (GP1BA Human)
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    G233V and M239V

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF1706

    Plasmid

    #143969

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGES401

    Plasmid

    #190198

    Purpose

    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5/FRT/TO_Lyn11-HaloTag7

    Plasmid

    #175533

    Purpose

    Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lyn11-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CGBE1 (pRZ3885)

    Plasmid

    #140252

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBXNH3CA_MBP

    Plasmid

    #132700

    Purpose

    expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Maltose binding Protein
    

    Use

    Tags

    Avi and His

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBad-Int Set Flipper

    Plasmid

    #38209

    Depositor

    Drew Endy

    Article

    Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

    Insert

    Bxb1 Integrase
    

    Use

    Synthetic Biology; Biobrick cloning vector

    Tags

    His

    Expression

    Mutation

    Promoter

    pBAD/AraC

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDestTol2-QUAS:GFP-CAAX

    Plasmid

    #184813

    Purpose

    Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:GFP-CAAX-pA
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    GFP-CAAX

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-EF1a-pMagHigh-FlpC

    Plasmid

    #180586

    Purpose

    PA-Flp

    Depositor

    Won Do Heo

    Article

    Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

    Insert

    Split FlpO
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTcI38-Wasabi

    Plasmid

    #86117

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI38
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    pR/pL and pR/pL, LacI

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. V245 pCEP-4HA B56alpha

    Plasmid

    #14532

    Depositor

    David Virshup

    Article

    Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

    Insert

    PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
    

    Use

    Tags

    4 HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLib6.4B-Aalb_743

    Plasmid

    #176676

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA5/FRT/TO_HaloTag7_LamB1

    Plasmid

    #175528

    Purpose

    Expression of HaloTag7 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag7-LamB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUPD2+p35SoTMV

    Plasmid

    #170876

    Purpose

    Phytobrick - p35SoTMV

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    p35SoTMV
    

    Use

    Tags

    Expression

    Plant

    Mutation

    214C>T and 229C>T to remove BbsI restrictio…

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEMB44 / Mouse_LRRK2_1_342

    Plasmid

    #40555

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Mutation

    WT

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pCA24N-sso7d-ligase

    Plasmid

    #87745

    Purpose

    IPTG-inducible expression of sso7d-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and codon optimized sso7d (Sulfolobus solfa…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge