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167,150 results

Showing: 11721 - 11760 of 167150 results

  1. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid
    #140976
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoA-RLuc8 (GNAO1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140976-plasmid-map-sequence-id-300659

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  3. AbVec2.0-IGHG4

    Plasmid
    #99578
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    99578-plasmid-map-sequence-id-192595

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  7. pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234439
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234439-plasmid-map-sequence-id-465689

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  8. PB53x EF1-Dendra2-RPB1Amr

    Plasmid
    #81228
    Purpose
    expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system
    Depositor
    Timothée Lionnet
    Article
    Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1
    Insert
    Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    N792D alpha amanitin resistance mutation (Bartolo…
    Promoter
    EF1a
    Available Since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    81228-plasmid-map-sequence-id-394051

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  21. pHN922

    Plasmid
    #178975
    Purpose
    Expression of GST-His8-GFP-nanobody
    Depositor
    Kazuhiro Abe
    Insert
    GST-His8-GFP-nanobody
    Use
    Affinity Reagent/ Antibody
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178975-plasmid-map-sequence-id-354968

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  25. pcDNA3-endo-Nb80

    Plasmid
    #205518
    Purpose
    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-Nb80
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205518-plasmid-map-sequence-id-409484

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Showing: 11721 - 11760 of 167150 results