We narrowed to 18,127 results for: Mut

1. pBabe hygro NFS1

   Plasmid

   #102977

   Purpose

   Expression of NFS1

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   NFS1 (NFS1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations to eliminate shNFS1_1 binding si…

   Promoter

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5 HA3-eDHFR-T2A-neo

   Plasmid

   #86396

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A neo
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A neo

   Expression

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Promoter

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK-flag-USP19 C506S

   Plasmid

   #78584

   Purpose

   Expression flag-USP19 C506S in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 C506S (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutate 506 cysteine residue to serine

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pDronpa-N1-Tau-μDpTT

   Plasmid

   #182323

   Purpose

   Microtubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   Tau-μDronpa-TagRFP-T
   

   Use

   Tags

   MAP tau

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK010

   Plasmid

   #69874

   Purpose

   expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

   Depositor

   Robert Kittel, Georg Nagel

   Article

   Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

   Insert

   Channelrhodopsin-2
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   D156C (XXL)

   Promoter

   Drosophila Hsp70

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-GFP-CtIP-T847E

   Plasmid

   #71111

   Purpose

   mammalian expession of GFP-CtlP T847E mutation

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T847E

   Promoter

   TRE promoter, Tet ON

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pcDNA3 S6K2 KR

   Plasmid

   #17730

   Depositor

   John Blenis

   Article

   Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

   Insert

   S6K2 (RPS6KB2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K99R: kinase dead mutant

   Promoter

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-IREShygro2

   Plasmid

   #153966

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   EGFP
   

   Use

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CMV promoter

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CL7-LbCas12aD156R

    Plasmid

    #164659

    Purpose

    Bacterial expression of mutated LbCas12aD156R using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a-D156R
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 156 to Arginine

    Promoter

    T7

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-Pidd1(T788A)

    Plasmid

    #60529

    Purpose

    Retroviral expression of Pidd1 T788A phosphorylation mutant

    Depositor

    Trudy Oliver

    Article

    Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

    Insert

    Pidd1 (PIDD1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Threonine 788 to Alanine*

    Promoter

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Fast-GCaMP-EF16

    Plasmid

    #52642

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D431N/D433N/D435N/E442A mutated from GCaMP3

    Promoter

    T7

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

13. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Promoter

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Fast-GCaMP-EF19

    Plasmid

    #52644

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF19
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T328D/T330D mutated from GCaMP3

    Promoter

    T7

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

15. CAGhP14

    Plasmid

    #84362

    Purpose

    express FLAG-HA tagged human PRDM14

    Depositor

    Danny Reinberg

    Article

    Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    silent mutations at coding silent 1068CtoT, 1629T…

    Promoter

    CAG

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

16. hPDI1-18-508_C400S_C56S-pFLAG-CMV1

    Plasmid

    #31383

    Depositor

    David Ron

    Article

    Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

    Insert

    PDI1A (P4HB Human)
    

    Use

    Tags

    FLAG M2

    Expression

    Mammalian

    Mutation

    C-terminal cysteines of both active sites mutated…

    Promoter

    Available Since

    May 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid

    #212120

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    

    Use

    Tags

    His6 and SUMO-TAG35

    Expression

    Bacterial

    Mutation

    E35TAG (within SUMO encoding sequence)

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MadMyc-HA

    Plasmid

    #16557

    Depositor

    Bert Vogelstein

    Article

    Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

    Insert

    MADMYC (MYC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative mutant

    Promoter

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pNC1125

    Plasmid

    #41561

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-GAL1-UBIMdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330A_D10A-1x7

    Plasmid

    #58777

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK-flag-USP19 1-1290

    Plasmid

    #78579

    Purpose

    Expression flag-USP19 1-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 1-1290 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 1291 residue to stop condon

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pDS277

    Plasmid

    #34973

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V529N

    Promoter

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST14-SpyCatcher002 EQ

    Plasmid

    #102830

    Purpose

    Bacterial expression of unreactive version of SpyCatcher002

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyCatcher002 EQ
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    E77Q mutation prevents isopeptide bond formation

    Promoter

    T7

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MAC_N_SOX10

    Plasmid

    #167791

    Purpose

    Expresses SOX10 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBS NL4-3 envFS eGFP RT-D185K/D186L

    Plasmid

    #101332

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    reverse transcriptase D185K/D186L

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. human p53-(1-393)-N268D

    Plasmid

    #24861

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Asparagine 268 mutated to Aspartate

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

27. MAC_N_NKX25

    Plasmid

    #167785

    Purpose

    Expresses NKX25 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    NKX25 (NKX2-5 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p35S-PBE

    Plasmid

    #119772

    Purpose

    Conversion of C to T in potato

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    NLS-APOBEC1-nCas9-UGI-NLS
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    CaMv 2x35S

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC-DTA-DEST-R3R4(R)

    Plasmid

    #141011

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    DTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLPC flag-mRAP1 I312R

    Plasmid

    #73138

    Purpose

    Expression of Flag-tagged mRAP1 with single point mutatoin I312R

    Depositor

    Agnel Sfeir

    Article

    Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

    Insert

    Rap1 (Terf2ip Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pCZGY2851

    Plasmid

    #87761

    Purpose

    Punc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Punc-17b-PH-miniSOG(Q103L)
    

    Use

    Tags

    PH

    Expression

    Worm

    Mutation

    Promoter

    Punc-17b

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA-Wnt2

    Plasmid

    #35906

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt2 (WNT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent Mutation G (816) --> A

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Fluorescent reporter for MOBE3-4

    Plasmid

    #219946

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AICSDP-144:CTCF-mEGFP

    Plasmid

    #168799

    Purpose

    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF

    Depositor

    Allen Institute for Cell Science

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    June 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. JARID1BA

    Plasmid

    #74646

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JARID1BA (KDM5B Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Expression

    Mutation

    Leu101-(Gly)4-Ala374 deletion mutant

    Promoter

    PH

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pUG-UBA1

    Plasmid

    #104665

    Purpose

    Cloning Module

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    AtUBA1 (UBA1 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BpiI/BsaI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNC1011

    Plasmid

    #42898

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    3 tandem copies of GFP*
    

    Use

    Yeast integrating

    Tags

    Expression

    Mutation

    changed Phenylalanine 64 to Leucine, Serine 65 t…

    Promoter

    None

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAIDFA-CMV-NmScarlet-mAID

    Plasmid

    #140616

    Purpose

    Expression of OsTIR1F74A and mScarlet-mAID protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1 mutated F74A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. YF-Rac1(DN)

    Plasmid

    #20151

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Rac1(DN) (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dominant negative mutation

    Promoter

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti-Arch-EEQ

    Plasmid

    #45188

    Purpose

    Arch voltage indicator with double mutations D95Q-D106E (Arch-EEQ), with faster kinetics and greater fluorescence dynamic range than Arch-D95N

    Depositor

    Mark Schnitzer

    Article

    Gong et al PLoS One. 2013 Jun 19;8(6):e66959.

    Insert

    enhanced Archaerhodopsin
    

    Use

    Lentiviral

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    D95Q, D106E

    Promoter

    CamKIIa

    Available Since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge