-
Plasmid#149283PurposeT-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA_TrunFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)
Plasmid#192123PurposeLentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA(MS2)-puro-barcode
Plasmid#89493PurposeCloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seqDepositorInsertsgRNA (with MS2 loop)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUDE1084
Plasmid#168500Purposeexpression of T7RNA polymerase and CRISPR endonunclease Fncas12aDepositorInsertsFncas12a
T7RNA polymerase
UseCRISPRTagsExpressionMutationPromoterTDH3 and TEF1Available sinceDec. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A2.0
Plasmid#99896PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
Plasmid#115216PurposeExpresses Pin2Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin2Cas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB22
Plasmid#122244PurposeLentivirus delivery for stable expression of AsCas12a-VPRDepositorInsertmammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
UseLentiviralTagsNLS and VPR activatorExpressionMutationPromoterEFsAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
S9_attL1-EGFP-C-term-attL2
Plasmid#128473PurposeEGFP entry cloneDepositorInsertEGFP entry clone
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI
Plasmid#115208PurposeExpresses PinCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPinCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-3'UTR-Tetra-com-vector
Plasmid#132553PurposeExpresses spCas9 and sgRNA scaffold with com aptamer replacing the TetraloopDepositorInsertsCas9
sgRNA scaffold with com replacing the Tetraloop
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)
Plasmid#192122PurposeLentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(c+iii)DepositorInsertpEF1a-anti-CD19scFV_CD28_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWp30
Plasmid#73857PurposepFUGW-EFSp-Cas9-P2A-ZeoDepositorInsertEFSp-Cas9-P2A-Zeo
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2xp53_RE
Plasmid#118053PurposeGBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR
Plasmid#118464Purposecp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity.DepositorInsertsapphireAKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
Plasmid#115207PurposeExpresses BzoCas13b from a SUMO-tagged bacterial expression constructDepositorInsertBzoCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D-gRNA2.1
Plasmid#167161PurposeExpress single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)
Plasmid#192124PurposepHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
P2-ECFP-pA (Construct 6)
Plasmid#55198PurposeSynthetic promoter responsive to gRNA2DepositorInsertmCerulean
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKeratin-miRFP670nano
Plasmid#127437PurposeKeratin labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertKeratin-miRFP670nano
UseTagsmiRFP670nanoExpressionMammalianMutationKeratin V282L - please see depositor comments bel…PromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUBC_HA_2xPP7-VP64
Plasmid#68418PurposeTransient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoterDepositorInsertPP7(deltaFG)
UseSynthetic BiologyTagsHA and VP64ExpressionMammalianMutationPP7: [delta]67_74 (non-aggregating)Promoterhuman UbCAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_h7SK
Plasmid#102842PurposeCan be used as PCR template for a STAgR reactionDepositorInsertgRNAScaffold_h7SKpromoter
UseAs pcr templateTagsExpressionMutationPromoterh7SKAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
TB:7xSMAD_RE:MiniP
Plasmid#133879Purpose7 copies the SMAD DNA binding motifDepositorInsert7 copies the SMAD DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU1/Sm/3' Box_(GLuc)_TOP1
Plasmid#68437PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.DepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U1Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-M-Gal4
Plasmid#84554PurposeMESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivatorDepositorInsertRap-MESA-FRB-20-M-Gal4
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC155-pCR8-sgExpression
Plasmid#49045PurposeU6-driven sgRNA expressing cassette on a gateway donor vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_mU6
Plasmid#102845PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_mU6
UsePcr template for stagr insertsTagsExpressionMutationPromotermU6Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro
Plasmid#192683PurposeLentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TB:6xAP-1_RE:MiniP
Plasmid#133884Purpose6 copies of the AP-1 binding motifDepositorInsert6 copies of the AP-1 binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode1
Plasmid#83678Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode1
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-GMRWhite
Plasmid#165907PurposeBlasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
Plasmid#163074PurposeEntry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)DepositorInsertGrx1-roGFP1-iEER
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Omega2
Plasmid#165861PurposeOmega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NES-MBP-cATOM(Ras)
Plasmid#209711PurposeMammalian expression of c-ATOM biosensor for HRas with nuclear export signalDepositorInsertNES-MBP-cATOM(Ras)
UseTagsNES-MBPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_TOP2
Plasmid#68435PurposeTransient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertTOP2 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[Spinach2]
Plasmid#68430PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Spinach2" aptamer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hNEUROD1 gRNA_1-MS2-Puro
Plasmid#192676PurposeLentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcriptionDepositorInsertHuman NEUROD1 activating gRNA #1 (NEUROD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV/MASC_(GLuc)_INT
Plasmid#68440PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_TOP1
Plasmid#68423PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-MiniWhite
Plasmid#165910PurposeBlasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin-AflII
Plasmid#106404PurposeEmpty guide RNA cloning vector with an AflII site added from Addgene 41824DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_hH1
Plasmid#102847PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_hH1
UsePcr template for stagr insertsTagsExpressionMutationPromoterU6Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgSetd2#1/Cre
Plasmid#89649PurposeExpresses a Setd2-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Setd2 (Setd2 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:Lucia:bgH
Plasmid#135293PurposeTranscriptional unit for constitutive expression of Lucia luciferase in mammalian cellsDepositorInsertCMV:Lucia:bGH
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141C2.0
Plasmid#99905PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro
Plasmid#192681PurposeLentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcriptionDepositorInsertMouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Alpha1spm
Plasmid#165866PurposeModified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE389
Plasmid#149287PurposeT-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_TrunFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-LumiScarlet_SV40:PuroR
Plasmid#183044PurposeExpresses FLAG-tagged LumiScarlet in mammalian cells.DepositorInsertLumiScarlet
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI
Plasmid#115220PurposeExpresses Pin3Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin3Cas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-mCherry-MiniWhite
Plasmid#165911PurposeBlasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only