We narrowed to 25,905 results for: Nes
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Plasmid#135090PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered x3
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pluc HYG 5’ TMV Ω
Plasmid#247491PurposePlasmid pluc 5′ TMV Ω contains the 67-nt TMV Ω translational enhancer placed upstream of Firefly luciferase, optimized for boosting expression in plant transient and stable expression assays.DepositorInsertTMV Ω enhancer sequence
UseLuciferaseTagsn/aExpressionBacterial and PlantPromoterCaMV 35SAvailable SinceJune 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pluc HYG 5’ CYSTM1
Plasmid#247492PurposePlasmid pluc 5′ CYSTM1 contains the full 110-nt Arabidopsis CYSTM1 5′ UTR upstream of Firefly luciferase, serving as a translational enhancer in plant transient assaysDepositorInsertCYSTM 1 (complete 5' UTR of CYSTM1 gene)
UseLuciferaseTagsn/aExpressionBacterial and PlantPromoterCaMV 35SAvailable SinceJune 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYihI-C-term K-R
Plasmid#233092PurposeExpression of GST-YihI with C-term lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with C-term lysines (K) mutated to arginine (R)
TagsGSTExpressionBacterialMutationC-term lysines (K) mutated to arginine (R)Available SinceMay 12, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
FUCas9Cherry
Plasmid#70182PurposeExpresses Cas9 with fluorescent Cherry reporterDepositorInserthumanised S.pyogenes cas9
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianPromoterhUbCAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYL156 (TRV RNA2)
Plasmid#148969PurposeModified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)DepositorInsertModified TRV RNA2
UseT-dna vectorMutationsee comments section belowAvailable SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
p16 nlsGPS2
Plasmid#228093PurposeExpression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)DepositorInsertnlsGPS2
Tags6xHis-T7 XpressTag and cMycExpressionPlantPromoterp16Available SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBabe HA-Runx1-neo
Plasmid#45815DepositorAvailable SinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-Blast-Puro
Plasmid#167186PurposeLentiviral expression of sgRNA with blasticidin and puromycin resistance genesDepositorInsertBlasticidin S deaminase
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
shCTL-mlpx-puro
Plasmid#65232Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
ExpressionMammalianPromoterPGKAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYihI-N+C-term K-R
Plasmid#233093PurposeExpression of GST-YihI with N+C-term lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with N+C-term lysines (K) mutated to arginine (R)
TagsGSTExpressionBacterialMutationGST-YihI with N+C-term lysines (K) mutated to arg…Available SinceMay 12, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-All K-R
Plasmid#233094PurposeExpression of GST-YihI with all lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with all lysines (K) mutated to arginine (R)
TagsGSTExpressionBacterialMutationGST-YihI with all lysines (K) mutated to arginine…Available SinceMay 9, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-N-term S-A
Plasmid#233096PurposeExpression of GST-YihI with N-term serines (S) mutated to alanine (A)DepositorInsertYihI with N-term serines (S) mutated to alanine (A)
TagsGSTExpressionBacterialMutationN-term serines (S) mutated to alanine (A)Available SinceMay 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
5013 TRV2-eTnpBc-reRNA4ChlH
Plasmid#251824PurposeEditing H subunit of magnesium chelatase (ChlH) genes in Nicotiana benthamianaDepositorInsertEnhanced variant of ISDra2 eTnpBc with guide targeting NbChlH with HDV at 3' end
TagsAtFDnlsExpressionPlantMutationN4Y/R110K/V192L/L222IAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…MutationP697S mutation in putative replicase. S239G and S…Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYB-Dual(mNG(yeast-opt))
Plasmid#216727PurposeDual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.DepositorInsertmNeonGreen
ExpressionBacterial and YeastPromoterpGAL1(yeast) and pT7-RNAP(bacteria)Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2 ER:ras
Plasmid#67844Purpose4-hydroxytamoxifen inducible ER:ras fusion proteinDepositorInsertER1a H-RasG12V fusion
ExpressionMammalianMutationG525R renders the HBD largely insensitive to 17ß‐…PromoterCMVAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet rtTA G72V SE Δsplice GP Luc
Plasmid#194328PurposeTetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightnessDepositorInsertLuciferase
UseLuciferaseExpressionMammalianMutationTet-responsive promotor related mutations: G72V i…PromoterTight tet-responsive promotorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Alt AlphaPS3
Plasmid#14070DepositorInsertDrosphila Alt Alpha PS3 Integrin (scb Fly)
ExpressionBacterialAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
JEV-ED3
Plasmid#251259PurposeTo express the Japanese encephalitis virus envelope protein domain III in E. coliDepositorInsertJEV-ED3
Tags6xHis, 11 aa linker, thrombin cleavage siteExpressionBacterialAvailable SinceApril 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
JEV-ED3_C5I
Plasmid#251260PurposeTo express the Japanese encephalitis virus envelope protein domain III with a five-isoleucine tag at the C-terminus in E. coliDepositorInsertJEV-ED3_C5I
Tags5xIle with a GG linker and 6xHis, 11 aa linker, t…ExpressionBacterialAvailable SinceApril 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
pKG01417_TRE-TagBFP-bGH-(EF1alpha-mRuby2-SV40)
Plasmid#252542PurposeInducible gene and constitutive reporter with convergent syntax, used to generate monoclonal PiggyBac-integrated linesDepositorInsertmRuby2
ExpressionMammalianPromoterhEF1a (human elongation factor 1-alpha), Tight TR…Available SinceApril 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-dt/CBA-EGFP-NLS
Plasmid#163918PurposeContains bidirectional reporter genes dtomato and nuclear-targeting EGFP.DepositorInsertdtomato and EGFP
UseLentiviralExpressionMammalianMutationAdded nuclear localization signal to EGFPAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS22
Plasmid#129710PurposeExpresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusionDepositorInsertEscherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
Tags10-Histidine tag cleavable with PreScission Prote…ExpressionBacterialMutationRssB E134G:E135S:E136G:E137SPromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
shCTL-mlpx-neo
Plasmid#65233Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
ExpressionMammalianPromoterPGKAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Comp AlphaPS3
Plasmid#14071DepositorInsertDrosphila Comp Alpha PS3 Integrin (scb Fly)
ExpressionBacterialAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-TIR1-HA
Plasmid#172610PurposeLentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cellsDepositorInsertTIR1
UseLentiviralTagsHAExpressionMammalianPromoterTet/ON Minimal CMV promoterAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shRUNX1 puro
Plasmid#45816DepositorAvailable SinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe DN-FOXO1-HA neo
Plasmid#45814DepositorAvailable SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe RFP-DN-FOXO1-HA neo
Plasmid#45813DepositorAvailable SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-WT
Plasmid#180212PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1 x3 (CBX1 Human)
Tags6xhis-tag and MBPExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-7CtoA-LIN-41
Plasmid#52726Purposeretroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationchanged cysteines 12, 15, 61, 66, 69, 91, and 94 …Available SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKG01416_TRE-TagBFP-bGH-EF1alpha-mRuby2-SV40
Plasmid#252541PurposeInducible gene and constitutive reporter with downstream tandem syntax, used to generate monoclonal PiggyBac-integrated linesDepositorInsertmRuby2
ExpressionMammalianPromoterhEF1a (human elongation factor 1-alpha), Tight TR…Available SinceApril 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pKG01418_(TRE-TagBFP-bGH)-EF1alpha-mRuby2-SV40
Plasmid#252543PurposeInducible gene and constitutive reporter with divergent syntax, used to generate monoclonal PiggyBac-integrated linesDepositorInsertmRuby2
ExpressionMammalianPromoterhEF1a (human elongation factor 1-alpha), Tight TR…Available SinceApril 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
Kif1bβ-pcDNA3
Plasmid#22534DepositorAvailable SinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-CCND3
Plasmid#172621PurposeExpresses HA-tagged cyclin D3 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VPR-Puro
Plasmid#99373Purpose3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)DepositorInsert(Sp)dCas9-VPR-P2A-Puro
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1aAvailable SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-MCS(extended)-Puro (empty vector)
Plasmid#172603PurposeEmpty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianMutationThe multiple cloning site (MCS) has been edited/e…Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AMBRA1-3xFLAG
Plasmid#172605PurposeExpresses 3xFLAG-tagged AMBRA1 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-CCND1
Plasmid#172649PurposeExpresses HA-tagged cyclin D1 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CCND1
Plasmid#172632PurposeExpresses untagged cyclin D1 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-CDK4
Plasmid#172612PurposeExpresses HA-tagged CDK4 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND2
Plasmid#172629PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-CDK2
Plasmid#172613PurposeExpresses HA-tagged CDK2 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND3
Plasmid#172623PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CCND1(T286A)
Plasmid#172631PurposeExpresses untagged cyclin D1(T286A) in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CDK6
Plasmid#172614PurposeExpresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
Plasmid#73796PurposepRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNADepositorInsertsdCas9-Mxi1
Tet Repressor
Structural gRNA for S pyogenes
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1, pRPR1(TetO), and pTef1Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TetON-Sox2
Plasmid#110280PurposeDoxycycline-inducible overexpression of Sox2DepositorInsertSRY (sex determining region Y)-box 2 (Sox2 Mouse)
UseLentiviralTagsNo fusion genesExpressionMammalianMutationWild typePromoterTREAvailable SinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-2xFLAG-mAID-AMBRA1 HDR donor template
Plasmid#172609PurposeDonor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 geneDepositorInsert2xFLAG-mAID-AMBRA1 (AMBRA1 Human, Synthetic)
UseCRISPR; Human targetingAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only