178,737 results
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Plasmid#74873PurposeGateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).DepositorTypeEmpty backboneExpressionPlantPromoterNo PromoterAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only
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p5E-gfap
Plasmid#75024PurposeCan be used to drive expression in zebrafish astrocytes.DepositorPublicationInsertglial fibrillary acidic protein promoter (gfap Zebrafish)
Use5' entry vector for multisite gateway three …PromotergfapAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB511
Plasmid#74853PurposeGateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
WN10150
Plasmid#80443PurposeExpresses dead (908A) AsCpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnuclease dead (908A) AsCpf1
TagsNLS-3xHAExpressionMammalianMutationnuclease dead (D908A)Available sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB433
Plasmid#74824PurposeGateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCP20T-tdTomato
Plasmid#78469PurposeBroad range bacterial fluorescence labelingDepositorInserttdTomato
ExpressionBacterialAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA AKT1
Plasmid#78778PurposeTo overexpress AKT1 in Mammalian CellsDepositorAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-EZH2-PGK-Puro-IRES-GFP
Plasmid#75125PurposeRetroviral expression plasmid encoding wild type human EZH2DepositorAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-gp78-FLAG
Plasmid#62370PurposeExpress C-terminally FLAG tagged gp78DepositorAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
alpha catenin conformation sensor
Plasmid#71709PurposeThis alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-catenin FRET Sensor
TagsECFP and YPETExpressionMammalianPromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGG-9.47_VP1,3 #2515
Plasmid#74237PurposeModified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47DepositorInsertsAAV2 Rep
AAV9.47 VP1
AAV9.47 VP3
UseAAVAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH
Plasmid#74289PurposepAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent mannerDepositorInsertH2B-GFP and oG (optimized Glycoprotein)
UseAAVExpressionMammalianMutationchimeric glycoproteinPromoterEf1aAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRExpressionBacterialPromoterpBADAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PMXS-SLC1A3
Plasmid#72873PurposeThe retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson AssemblyDepositorAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-LAMP1-FLAG
Plasmid#71868PurposeoverexpressionDepositorAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-mCherry-LacR-BRCA1
Plasmid#71115Purposemammalian expression of mCherry-LacR-BRCA1 siRNA resistant constructDepositorAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHBV-Luc
Plasmid#71414PurposeTo see the activity of HBV transcription using Luciferase assayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHBV core promoter, enhancer I, enhancer II and luciferase
UseLuciferaseExpressionMammalianPromoterHBVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Notch1 intracellular domain-pcw107-V5
Plasmid#64622Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-IRF9
Plasmid#71452PurposeLentiviral expression of IRF9DepositorAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmGFP10C-Tau
Plasmid#71433PurposeSplit GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTau (0N4R) (MAPT Human)
TagsGFP (1-212)ExpressionMammalianAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5E-mfap4
Plasmid#70052Purpose5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.DepositorInsertmfap4 promoter
UseGateway 5' elementAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
V48 ELP
Plasmid#68395PurposeElastin-like polypeptide (VPGxG) with 48 repeats of valine guest residueDepositorInsertV48 ELP
ExpressionBacterialPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GPR120-Tango
Plasmid#66310PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR120 (FFAR4 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pETM6-E12-vioABECD
Plasmid#66537PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)DepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyExpressionBacterialPromoterMutant T7 Promoter - G6 and Mutant T7 Promoter - …Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 Flag C/EBPb LAP
Plasmid#66979PurposeExpression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of proteinDepositorAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP
Plasmid#62348PurposeAn empty optimized gRNA expression vectorDepositorInsertnone
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKII-Jaws-KGC-GFP-ER2
Plasmid#65015PurposeAAV-mediated expression of Jaws under the CaMKII promoterDepositorInsertJaws-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterCaMKIIAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
th-P2A-EGFP donor
Plasmid#65562PurposeIntron targeting-mediated EGFP knockin at the zebrafish th locus.DepositorAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV2-SEP-GluA1 (M1)
Plasmid#64942Purposemammalian expression of rat GluA1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGluA1 (Gria1 Rat)
TagsSEP (superecliptic pHluorin)ExpressionMammalianPromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AR-V7-pcw107
Plasmid#64635Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertAR (transcript variant 1, splice isoform) (AR Human)
UseLentiviralMutationsplice isoform*PromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by