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171,784 results

Showing: 9391 - 9420 of 171784 results

  1. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and EF1-a
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  5. pExp-Lipo

    Plasmid
    #129237
    Purpose
    Protein expression in E.coli
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    His and Strep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129237-plasmid-map-sequence-id-252322

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  11. pSBbi-Hyg

    Plasmid
    #60524
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60524-plasmid-map-sequence-id-456411

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  19. pAG Lenti CMV Empty Puro

    Plasmid
    #236083
    Purpose
    Empty lentiviral backbone
    Depositor
    Melina Fan
    Type
    Empty backbone
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236083-plasmid-map-sequence-id-467823

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  23. pCALNL-GFP

    Plasmid
    #13770
    Purpose
    Cre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10
    Insert
    EGFP
    Use
    Cre/Lox
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 20, 2007
    Availability
    Academic Institutions and Nonprofits only
    13770-plasmid-map-sequence-id-7759

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  25. pLPT234

    Plasmid
    #127855
    Purpose
    Repressilator 2.0, without degradation tags, integrated triple reporter
    Depositor
    Johan Paulsson
    Article
    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:
    Inserts
    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    First 11aa derived from mCherry as fusion protein
    Promoter
    pAmpR, pL LacO1, pL tetO1, and pR
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    127855-plasmid-map-sequence-id-253039

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  29. pOCC175

    Plasmid
    #118890
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; mGFP, cleav…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118890-plasmid-map-sequence-id-232288

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Showing: 9391 - 9420 of 171784 results