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Addgene

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8,626 results

Showing: 1701 - 1750 of 8626 results

  1. pRMC2

    Plasmid
    #68940
    Purpose
    tetracycline-inducible expression vector for Staphylococcus aureus
    Depositor
    Tim Foster
    Article
    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1
    Type
    Empty backbone
    Use
    Tet inducible e. coli/staphylococcus shuttle vect…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    68940-plasmid-map-sequence-id-171245

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  3. pYD1

    Plasmid
    #73447
    Purpose
    expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells
    Depositor
    Dane Wittrup
    Article
    Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.
    Type
    Empty backbone
    Use
    Tags
    6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    73447-plasmid-map-sequence-id-364550

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  9. Dronpa3-N1

    Plasmid
    #54682
    Purpose
    Localization: N1 Cloning Vector, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    Dronpa3
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54682-plasmid-map-sequence-id-139033

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  10. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid
    #50474
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50474-plasmid-map-sequence-id-471606

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  12. HOP-flash

    Plasmid
    #83467
    Purpose
    TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene
    Depositor
    Barry Gumbiner
    Article
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Insert
    Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83467-plasmid-map-sequence-id-350804

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  19. pdCas9-bacteria

    Plasmid
    #44249
    Purpose
    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 (bacteria)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    pLtetO-1
    Available since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    44249-plasmid-map-sequence-id-269588

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  20. pSBbi-Bla

    Plasmid
    #60526
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60526-plasmid-map-sequence-id-97520

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  27. pCDH-EF1

    Plasmid
    #72266
    Purpose
    Express gene of interest under EF-1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72266-plasmid-map-sequence-id-447561

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  30. pCW57.1_V5_TurboID_FLAG_Nt

    Plasmid
    #194072
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    V5-TurboID-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194072-plasmid-map-sequence-id-380714

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  35. pSBbi-pur H-2Kb

    Plasmid
    #111623
    Purpose
    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele
    Depositor
    Jon Yewdell
    Insert
    H2Kb
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111623-plasmid-map-sequence-id-213220

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  37. pgRNA-humanized

    Plasmid
    #44248
    Purpose
    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    guide RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44248-plasmid-map-sequence-id-190219

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  40. pcDNA3.1-CMV-CFP;UBC-Cre25nt

    Plasmid
    #65727
    Purpose
    Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.
    Depositor
    Jacco van Rheenen
    Article
    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1
    Inserts
    CMV promoter driving CFP expression
    UBC promoter driving Cre-25nt expression
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and UBC
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    65727-plasmid-map-sequence-id-243566

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  43. pC0043-PspCas13b crRNA backbone

    Plasmid
    #103854
    Purpose
    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6-BbsI-BbsI-PspCas13b DR-polyT
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    103854-plasmid-map-sequence-id-350782

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Showing: 1701 - 1750 of 8626 results