159,117 results

1. pLentiCMV-Blast

   Plasmid

   #125133

   Purpose

   Lentiviral empty backbone control vector with CMV promoter and Blasticidin resistance gene

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-teLuc c-myc

   Plasmid

   #100026

   Purpose

   Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   teLuc, a NanoLuc mutant
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   D19S, D85N, C164H

   Promoter

   CMV

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEG BacMam N term StrepII eGFP 3C

   Plasmid

   #160683

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   StrepII eGFP 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR8 JARID2

   Plasmid

   #114443

   Purpose

   Entry vector for gateway cloning containing human JARID2 coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   JARID2 (JARID2 Human)
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

   Plasmid

   #221233

   Purpose

   Express sgRNA targeting HEK3 loci with nCas9(D10A)

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(D10A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDFDuet-1::ADAT3-ADAT2

   Plasmid

   #15917

   Depositor

   Nina Papavasiliou

   Article

   Rubio et al Proc Natl Acad Sci U S A. 2007 May 8. 10

   Insert

   ADAT3 & ADAT2
   

   Use

   Tags

   FLAG and HexaHistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825)

   Plasmid

   #214862

   Purpose

   Expression of iCre(R297T) in striatal medium spiny neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Has Service

   AAV PHP.eB

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

   Plasmid

   #105921

   Purpose

   AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs

   Depositor

   Marcella Patrick

   Has Service

   AAV2

   Inserts

   mKate2
   IRES-Multiple cloning site
   

   Use

   AAV

   Tags

   Expression

   Mutation

   codon optimized

   Promoter

   CBh and same CBh promoter as mKate2

   Available since

   Feb. 15, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. BRCA2 A11.2 gRNA

   Plasmid

   #90550

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA2 (Guide Designation A11.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-AID-BLM-UGI-T2A-mTagBFP2

    Plasmid

    #221222

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-BLM-UGI-T2A-mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. EBFP2-H2B-6

    Plasmid

    #55243

    Purpose

    Localization: Nucleus/Histones, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. AICSDP-19:LAMP1-mEGFP

    Plasmid

    #101782

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1

    Depositor

    Allen Institute for Cell Science

    Insert

    LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAAV-gfaABC1D-mCherry-CAAX

    Plasmid

    #218189

    Purpose

    To over express mCherry-CAAX under gfaABC1D promoter in astrocytes

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    mCherry-CAAX
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. H2B-GFP

    Plasmid

    #11680

    Depositor

    Geoff Wahl

    Article

    Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

    Insert

    Human histone H2B (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. C1-MPAct-mRuby3

    Plasmid

    #155221

    Purpose

    Monitor changes in the density of membrane-proximal F-actin (MPA)

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAD-LyseR

    Plasmid

    #99244

    Purpose

    For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

    Depositor

    Jeff Hasty

    Article

    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

    Insert

    phage lambda gene R (R phage lambda)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    beta-lactamase promoter

    Available since

    Oct. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189923

    Purpose

    AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e V106W
    

    Use

    AAV

    Tags

    Expression

    Mutation

    nSaCas9 KKH, TadA ABE8e V106W

    Promoter

    EFS

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_V5-TWIST1

    Plasmid

    #215030

    Purpose

    Mammalian expression of human TWIST1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    twist family bHLH transcription factor 1 (TWIST1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDSG372

    Plasmid

    #115602

    Purpose

    surface-displayed Nb1; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-MATR3

    Plasmid

    #32880

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDsRed-importinα

    Plasmid

    #119679

    Purpose

    Expresses DsRed-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

    Insert

    importinα (KPNA2 Human)
    

    Use

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Contains amino acids 251-529

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFH2.2_mCherry-MS2x12

    Plasmid

    #205521

    Purpose

    Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pACS129

    Plasmid

    #204594

    Purpose

    Histone H3.1-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    histone H3.1 (H3C3 Human)
    

    Use

    Lentiviral

    Tags

    human histone H3.1-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pICH47742::p35S-PE2max-NC::tEURb7

    Plasmid

    #227693

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. N174-MCS (Puro)

    Plasmid

    #81068

    Purpose

    Lentiviral vector for constitutive gene expression.

    Depositor

    Adam Karpf

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AICSDP-8:TOMM20-mEGFP

    Plasmid

    #87423

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. pGEMS-II-PEST

    Plasmid

    #204766

    Purpose

    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    EGFP-PEST-m6Alinker-DHFR
    

    Use

    Tags

    Expression

    Mutation

    mutated all RACs in EGFP to RGC/RCC

    Promoter

    CMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHCMV-EcoEnv

    Plasmid

    #15802

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    MLV env (ecotropic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. P4-TadCBEd

    Plasmid

    #225143

    Purpose

    Packaging plasmid for TadCBEd in v3b PE-eVLP architecture

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    P4-TadCBEd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mEmerald-Calreticulin-N-16

    Plasmid

    #54023

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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