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Plasmid#179550Purposeencodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
dead Sa Cas9
Plasmid#138162PurposeFor orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)DepositorInsertdSaCas9
UseTagsNLSExpressionMammalianMutationD10A + N580APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB-SaCas9
Plasmid#105998PurposeSaCas9 expression vectorDepositorInsertCas9 nuclease
UseCRISPR and LentiviralTagsnoneExpressionMammalianMutationnonePromotershort EF1alphaAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-J23111
Plasmid#113149PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A & H840APromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
HiFi Cas9
Plasmid#188490PurposeExpresses FLAG tagged HiFi Cas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationR691APromoterEF1aAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-spCas9-SB
Plasmid#169919PurposeExpression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.DepositorInsertSpCas9-SB100X
UseCRISPRTagsExpressionMutationPromoterCAGAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p246 eSpCas9_2gRNAs_mH11
Plasmid#164854PurposegRNA vector for targeting mouse H11 locusDepositorInsertgRNAs for targeting mouse H11 locus
UseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3
Plasmid#90087PurposeExpresses wild type caspase 3 in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationWild typePromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
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nCas9-PPE
Plasmid#140445PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6c
Plasmid#215821PurposeExpress CBE6c (with SpCas9) in mammalian cellsDepositorInsertCBE6c-SpCas9-2xUGI (cas9 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-enAsCas12f
Plasmid#204637PurposeExpression of enAsCas12f in mammalian cellsDepositorInsertenAsCas12f
UseTagsNLSExpressionMammalianMutationD196K, N199K, G276R, N328G, D364RPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9(D10A)
Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyTagsExpressionBacterialMutationD10APromoterpTetoAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSP3545-dCas9Str
Plasmid#153516PurposeExpresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backboneDepositorInsertdCas9Str
UseTagsExpressionBacterialMutationPromoternisAAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p201G Cas9
Plasmid#59178PurposeCas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
sGFP
UseCRISPRTagsExpressionMutationPromoter2x35SAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-CjCAS9-pA
Plasmid#131543PurposeExpresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCjCas9
UseCRISPRTagsFLAG-SV40NLS, SV40NLS, and polyAExpressionMammalianMutationHuman codon-optimizedPromoterCAG, T3Available sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Cas9-hGem_gRNA2
Plasmid#217659Purposeexpresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locusDepositorInsertsgRNA2 targeting the human AAVS1 safe harbour locus
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-nuCPE
Plasmid#213748PurposeFor circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cellsDepositorInsertMCP-LbCas12a-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R in LbCas12aPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas12a
Plasmid#158713PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinumDepositorInsertthe MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only