161,242 results

1. pDONR221-TAF15 Stop

   Plasmid

   #84895

   Purpose

   DONOR vector for Gateway cloning of TAF15

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Gateway donor

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. M57 pPET28a-TEV-full-length human beta catenin

   Plasmid

   #17198

   Depositor

   Randall Moon

   Insert

   beta catenin (CTNNB1 Human)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1his p66shc (corrected)

   Plasmid

   #32574

   Depositor

   Toren Finkel

   Article

   Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

   Insert

   p66shc (SHC1 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAKlux2.1

   Plasmid

   #14081

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSpCas9_T2A_mCherry sg1-sg2

   Plasmid

   #192479

   Purpose

   All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-PE7 for IVT

   Plasmid

   #214813

   Purpose

   Template for in vitro transcription of PE7

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Promoter

   T7 (inactivated)

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYUB28b

   Plasmid

   #37277

   Depositor

   Ted Baker

   Article

   Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

   Type

   Empty backbone

   Use

   Mycobacteria shuttle vector

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. FEMP

   Plasmid

   #191973

   Purpose

   FRET-based indicator of ER-mitochondria proximity (FEMP)

   Depositor

   Luca Scorrano

   Article

   Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

   Insert

   AkapYFP-Tav2a-CFP-sac1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-FLAG-Synaptojanin 1-170

   Plasmid

   #22292

   Depositor

   Pietro De Camilli

   Article

   Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

   Insert

   Synaptojanin 1_170 (SYNJ1 Human)
   

   Use

   Tags

   Flag

   Expression

   Bacterial and Mammalian

   Mutation

   K334R compared to Genbank ID NM_003895

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DECOR

    Plasmid

    #219545

    Purpose

    Expression of CMV-TadA8e-NLS-dCas13d-NLS in mammalian cells (DECOR expression)

    Depositor

    Weixin Tang

    Article

    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

    Insert

    DECOR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LT3GEPIR-CCND1shRNA2

    Plasmid

    #220578

    Purpose

    To inducibly knockdown CCND1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

    Insert

    CCND1 shRNA2 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICE-EGFP-FLAG-PSMD2

    Plasmid

    #161917

    Purpose

    Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA

    Plasmid

    #89459

    Purpose

    constitutive expression of human CHD7 in mammalian cells

    Depositor

    Peter Scacheri

    Insert

    CHD7 (CHD7 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. T777T

    Plasmid

    #113082

    Purpose

    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223-FES

    Plasmid

    #23876

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FES (FES Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pGEX_hDDX5_FL_wt

    Plasmid

    #88871

    Purpose

    inducible expression of GST-DDX5 in bacteria

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pD649-HAsp-SEMA4A-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156938

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA4A (SEMA4A Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FLAG-Synaptojanin 1-145

    Plasmid

    #22291

    Depositor

    Pietro De Camilli

    Article

    Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

    Insert

    Synaptojanin 1_145 (SYNJ1 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-tTA

    Plasmid

    #175280

    Purpose

    AAV vector mediating neuron-specific expression of tTA

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    tTA advanced (improved tetracycline-controlled transactivator)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-TagRFP

    Plasmid

    #67167

    Purpose

    CRISPaint universal donor for TagRFP fusion tagging

    Depositor

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    TagRFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pfmh-hCry1

    Plasmid

    #25843

    Depositor

    Aziz Sancar

    Insert

    human Cryptochrome 1 (CRY1 Human)
    

    Use

    Tags

    flag, his, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

22. FLAG-HA-FbxO5-pcDNA3.1-

    Plasmid

    #52509

    Purpose

    expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO5 (FBXO5 FbxO5, Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pmCherry-Synaptojanin-1-145

    Plasmid

    #223563

    Purpose

    Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

    Depositor

    Pietro De Camilli

    Article

    Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Use

    Tags

    His and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pINDUCER20-FLAG-hMYOD1 (#800)

    Plasmid

    #78330

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMyNT

    Plasmid

    #42191

    Depositor

    Annabel Parret, Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    ColE1

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid

    #106964

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE-ESR1-Y537S

    Plasmid

    #116374

    Purpose

    Lentiviral expression of ESR1 Y537S

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Y537S

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGGS-mTagBFP2-T2A-iCre-ERT2

    Plasmid

    #128168

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAPGSTMA N-terminus)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TC1332

    Plasmid

    #164874

    Purpose

    CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    hAPOBEC3A fused to PspCas13b (APOBEC3A )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human apobec Y132D

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBJ7

    Plasmid

    #167136

    Purpose

    A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge