165,256 results

1. hGli3 flag3x

   Plasmid

   #84921

   Purpose

   mammalian expression of Gli3

   Depositor

   Martin Fernandez-Zapico

   Insert

   Gli3 (GLI3 Human)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p18sheLL

   Plasmid

   #37321

   Depositor

   John Schiller

   Article

   Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

   Insert

   HPV18L1+L2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF3-ultraID

   Plasmid

   #172878

   Purpose

   expresses ultraID in mammalian cells with the tet-on or tet-off system

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   ultraID
   

   Use

   Luciferase and Retroviral; To be used in a tet-on…

   Tags

   myc

   Expression

   Mammalian

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   TRE-cmv

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Sport6-CD9-pHluorin

   Plasmid

   #130905

   Purpose

   Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD9-pHluorin (CD9 Human, Aequorea victoria)
   

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   phluorin inserted between Asn-50 and Asn-51 in ex…

   Promoter

   CMV

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. wtTDP43tdTOMATOHA

   Plasmid

   #28205

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Tags

   HA tag and tdtomato

   Expression

   Mammalian

   Available Since

   June 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-hAR

   Plasmid

   #89078

   Purpose

   mammalian expression of human androgen receptor in pCMV5

   Depositor

   Elizabeth Wilson

   Article

   Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

   Insert

   human androgen receptor (hAR) full-length (AR Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBlueSKMimRNA

   Plasmid

   #102535

   Purpose

   Template for synthesis of Minos transposase mRNA

   Depositor

   Michalis Averof

   Article

   Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

   Insert

   Minos transposase mRNA
   

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-GFPM2

   Plasmid

   #102791

   Purpose

   Expresses GFP under WT T7 promoter

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   Green Fluorescent Protein
   

   Expression

   Bacterial

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHD-DsRed

   Plasmid

   #51434

   Purpose

   Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

   Depositor

   Kate O'Connor-Giles

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Insert

   LoxP-3xP3-DsRed-LoxP
   

   Promoter

   3xP3

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Sport6-CD81-pHluorin

    Plasmid

    #130903

    Purpose

    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHluorin (CD81 Human, aequorea victoria)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Leu-49 and Gly-50 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. His6-SpyCatcher-mi3

    Plasmid

    #113043

    Purpose

    Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

    Insert

    His6-SpyCatcher-mi3
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYLCRISPR/Cas9Pubi-H

    Plasmid

    #66187

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVSVΔG-eGFP-linker

    Plasmid

    #231707

    Purpose

    Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV antigenome
    

    Use

    Vsv expression

    Expression

    Mammalian

    Mutation

    ΔG (VSV glycoprotein removed)

    Promoter

    T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-R2Tg_opt-3xFlag

    Plasmid

    #223251

    Purpose

    Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-N-HA-NEK7

    Plasmid

    #75142

    Purpose

    Expresses N-terminal HA-tagged NEK7 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Caspase1-flag

    Plasmid

    #21142

    Depositor

    Junying Yuan

    Article

    Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

    Insert

    caspase-1 (Casp1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223249

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-EF1Alpha-Tet-on 3G

    Plasmid

    #118592

    Purpose

    Expresses Tet-on 3G from the EF1Alpha promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Tet-on 3G
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-EGFP-Tau AP

    Plasmid

    #46905

    Purpose

    expresses EGFP tagged Tau AP in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau AP (MAPT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    all 14 S/P or T/P amino acid residues (T111, T153…

    Promoter

    CMV

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBudORF1-CH

    Plasmid

    #51290

    Purpose

    expresses the codon optimized ORF1 of L1RP with his tag

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1 ORF1 (L1RE1 Human)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRK5_mEGFP-NUP98-HOXA9

    Plasmid

    #237639

    Purpose

    For overexpression of mEGFP-NUP98-HOXA9

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NUP98-HOXA9 (NUP98, HOXA9 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Expression

    Bacterial

    Promoter

    native promoter

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLib6.4

    Plasmid

    #133783

    Purpose

    Library backbone

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pB-TopFlash-NanoLucP

    Plasmid

    #204347

    Purpose

    TopFlash reporter

    Depositor

    Li-Fang Chu

    Article

    Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

    Insert

    TopFlash-NanoLucP
    

    Use

    Luciferase

    Tags

    NanoLucP

    Promoter

    miniP

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. Ac5-STABLE1-neo

    Plasmid

    #32425

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Insert

    GFP-T2A-neo
    

    Expression

    Insect

    Promoter

    Actin5c

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCAFNF-GFP

    Plasmid

    #13772

    Purpose

    Flp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    EGFP
    

    Use

    Flp/frt

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-Neo_CAG-Cas9

    Plasmid

    #86698

    Purpose

    Cas9 expression vector for targeting to the AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    pCag

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCMV5B-TGFbeta receptor II wt

    Plasmid

    #11766

    Depositor

    Jeff Wrana, Joan Massague

    Article

    Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

    Insert

    TGFbeta receptor II (TGFBR2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. Lenti-dCas9-KRAB-blast

    Plasmid

    #89567

    Purpose

    Plasmid expression dCas9 protein in fusion with KRAB domain

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    Blasticidin resistance gene and KRAB

    Mutation

    D10A and H840A

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pGEX6P1-mCherry-Nanobody

    Plasmid

    #70696

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    mCherry-Nanobody
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    March 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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