165,688 results

1. pLV-Kif5b-T92N-Myc

   Plasmid

   #186615

   Purpose

   Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B Rigor mutant (T92N) fused to Myc tag

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Kinesin Heavy Chain isoform 5b Rigor mutant (T92N) (KIF5B Human)
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   T92N

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEP201-Lenti-TRE3G-Flag-GluN2B-WPRE-pA

   Plasmid

   #164775

   Purpose

   pLenti vector backbone designed to express Flag-GluN2B from a TRE3G promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2B
   

   Use

   Lentiviral

   Tags

   Flag

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-XPO5-His6

   Plasmid

   #171562

   Purpose

   Encodes human XPO5 protein

   Depositor

   Yan Zeng

   Insert

   XPO5 (XPO5 Human)
   

   Expression

   Bacterial

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGA2ox2:GUS

   Plasmid

   #108564

   Purpose

   Arabidopsis transformation, expresses GA2ox2 promoter/GUS fusion to study expression pattern in roots

   Depositor

   Ton Bisseling

   Article

   Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

   Insert

   GA2ox2 promoter (GA2OX2 Mustard Weed)
   

   Tags

   GUS

   Expression

   Plant

   Promoter

   GA2ox2

   Available Since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-ET-F656W

   Plasmid

   #72546

   Purpose

   expresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W mutation

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   BRD4 ET (BRD4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F656W

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-ET-I654Q

   Plasmid

   #72545

   Purpose

   expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutation

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   BRD4 ET (BRD4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   I654Q

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-ET-L630W

   Plasmid

   #72543

   Purpose

   expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W mutation

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   BRD4 ET (BRD4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L630W

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE HA Chk2 KD, dominant negative chk2

   Plasmid

   #41902

   Depositor

   Stephen Elledge

   Article

   Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

   Insert

   Chk2 (CHEK2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D347A

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

   Plasmid

   #107313

   Purpose

   Encoding aCamkII-mCherry-Cre

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-mCh-Cry2olig

    Plasmid

    #101222

    Purpose

    Cry2olig(E490G)

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2olig
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    SFFV

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

    Plasmid

    #80840

    Purpose

    Modified helper phage

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene II
    M13 gene X
    M13 gene V
    M13 gene VII
    M13 gene IX
    M13 gene VIII
    M13 gene I
    M13 gene XI
    M13 gene IV
    

    Expression

    Bacterial

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBACgus4x/cdc37/LRRK2 1867-2186

    Plasmid

    #40364

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    HisTev

    Mutation

    WT aa1867-2186

    Promoter

    polH

    Available Since

    Oct. 2, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pN251

    Plasmid

    #188471

    Purpose

    Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    enCas12a-HF (gift from Dr. Keith Joung)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXspuro-GFP

    Plasmid

    #74203

    Purpose

    Retroviral control vector containing GFP insert

    Depositor

    Jayanta Debnath

    Article

    Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

    Insert

    GFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSIQ-EGFP

    Plasmid

    #216239

    Purpose

    All-in One IQ transgene switch for EGFP expression

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. mKeima-Red-N1

    Plasmid

    #54597

    Purpose

    Localization: N1 Cloning Vector, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mKeima-Red

    Expression

    Mammalian

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. SIRT3L-12

    Plasmid

    #13736

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT3 (SIRT3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL-SH3(2)-5R

    Plasmid

    #112089

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-5R (NCK1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only repeats of the second SH3…

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA-FLAG-METTL3-APPA

    Plasmid

    #160251

    Purpose

    Express catalytic inactive FLAG-METTL3 in human cells

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changes in aa 395–398, DPPW → APPA

    Promoter

    CMV

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pIND-STAT1-V5

    Plasmid

    #11618

    Depositor

    Steven Johnson

    Insert

    Signal transducer and activator of transcription 1 (STAT1 Human)
    

    Use

    Ecdysone inducible

    Tags

    V5

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pDJSVT87

    Plasmid

    #109302

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pHM066

    Plasmid

    #225524

    Purpose

    Encodes Wnt-responsive destablized iRFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Insert

    iRFP
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. iTol2Amp-γ-crystallin:RFP

    Plasmid

    #108455

    Purpose

    This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

    Insert

    Tol2-Amp-y-cryst-mCherry
    

    Use

    Gateway

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pClneoMyc-LATS2

    Plasmid

    #66852

    Purpose

    Express Myc-tagged LATS2 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS2 (LATS2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCAGGs-BFP

    Plasmid

    #127348

    Purpose

    Expresses BFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Blue Fluorescent Protein
    

    Expression

    Mammalian

    Promoter

    CAGGS Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-hSyn1-mTurquoise2

    Plasmid

    #99125

    Purpose

    An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mTurquoise2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. GCH1_pLX307

    Plasmid

    #98336

    Purpose

    Lentiviral expression of GCH1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    GCH1 (GCH1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pOPINE GFP nanobody:Halo:His6

    Plasmid

    #111090

    Purpose

    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

    Depositor

    Lennart Wirthmueller

    Article

    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

    Insert

    GFP Nanobody
    

    Tags

    Halo and His6

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7 promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCaggs-mGold2s

    Plasmid

    #231763

    Purpose

    Expression of mGold2s in mammalian cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s
    

    Expression

    Mammalian

    Mutation

    mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Actin promoter with CMV enhancer

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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