163,445 results

1. pcDNA GASTM-3-RVG-10-Lamp2b-HA

   Plasmid

   #71295

   Purpose

   Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   GASTM mutated glycosylation motif, HA, Lamp2 sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-EcoRV

   Plasmid

   #165548

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   EcoRV
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. PB-UBC-GCAMP6f-PGK-neo

   Plasmid

   #160049

   Purpose

   expression of GCaMP6f in a piggybac plasmid

   Depositor

   Xiaojun Lian

   Insert

   GCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEGFP-RhoA-C1

   Plasmid

   #29674

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-CLIP-PD-L2

   Plasmid

   #223587

   Purpose

   Lentiviral expression of human PD-L2 with CLIP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   PD-L2 (PDCD1LG2 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   CLIP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-HF1

   Plasmid

   #138556

   Purpose

   Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-HF1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-HA-AR-NTD-DBD

   Plasmid

   #171236

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   AR truncate

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Plasmid L5 attB::Pleft* mWasabi

   Plasmid

   #169409

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mWasabi
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCoofy27

   Plasmid

   #44003

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   PPH

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSyn.iGluSnFR3.v857.IgK-NGR

    Plasmid

    #196227

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn iGluSnFR3 v857.NGR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and NGR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. hAAVS1 TALEN Left

    Plasmid

    #52341

    Purpose

    TALEN vector for genomic targeting

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    AAVS1 TALEN Left
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-D12L

    Plasmid

    #89161

    Purpose

    Express vaccinia virus D12L in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D12L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promotor

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304_zeo_mmIrf1

    Plasmid

    #160098

    Purpose

    Expresses murine Irf1 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Irf1 (Irf1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCV020-tetQ

    Plasmid

    #232826

    Purpose

    Random transposon mutagenesis vector for Bacteroidales species - tetracycline

    Depositor

    Athanasios Typas

    Insert

    tetQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJ241-FLEX

    Plasmid

    #18925

    Purpose

    FLEX switch empty backbone

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    FLEX switch
    

    Use

    Cre/Lox; Subcloning

    Tags

    Expression

    Mutation

    An internal Spe1 site is used for subcloning a ge…

    Promoter

    Available since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pED9x (dCas9-KRAB-mCherry)

    Plasmid

    #163956

    Purpose

    Lentiviral expression plasmid of sgRNA with mCherry

    Depositor

    Junwei Shi

    Article

    Giles et al Immunity. 2022 Mar 8;55(3):557-574.e7. d

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRosa26-DEST

    Plasmid

    #21189

    Purpose

    Rosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in transgenic mice

    Depositor

    Peter Hohenstein, Nick Hastie

    Article

    Hohenstein et al Pathogenetics. 2008 . 1(1):3.

    Insert

    GateWay conversion cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. 7xE-Box::Renilla

    Plasmid

    #124532

    Purpose

    c-Myc Renilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    c-Myc Renilla reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV- δLight

    Plasmid

    #201224

    Purpose

    Express deltaLight biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    δLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUGW-d2GFP-200

    Plasmid

    #79602

    Purpose

    FUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sites

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    d2GFP + miR-200 family targets
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. ABE8.8-m

    Plasmid

    #136294

    Purpose

    expresses ABE8.8-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.8-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pVoPo-mCh

    Plasmid

    #187841

    Purpose

    Constitutive mCherry-expression vector for Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. TrpC5 scFv [N67/15]

    Plasmid

    #190564

    Purpose

    Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSL2680

    Plasmid

    #85581

    Purpose

    Replicating base vector for constructing cpf1/CRISPR editing plasmids

    Depositor

    Himadri Pakrasi

    Article

    Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pALB-GFP

    Plasmid

    #55759

    Purpose

    Fluorescent marker for hepatocytic lineage

    Depositor

    Snorri Thorgeirsson

    Article

    Heo et al Hepatology. 2006 Dec;44(6):1478-86.

    Insert

    albumin enhancer/promoter (Alb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Albumin Enhancer/Promoter

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCAG-HA-rat Nrxn1alpha

    Plasmid

    #58266

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 1alpha

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn1alpha (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    see comments

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDD425

    Plasmid

    #202078

    Purpose

    Frame +0 selector for NHEJ knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Frame +0 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Plasmid

    #163075

    Purpose

    Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    roGFP2-Orp1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET28b-zDjCas13d-His

    Plasmid

    #234481

    Purpose

    Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    DjCas13d
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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