We narrowed to 561 results for: HR2

1. RabV CVS-N2c(deltaG)-hChR2-YFP

   Plasmid

   #73465

   Purpose

   Expresses channelrhodopsin fused to YFP for monosynaptic circuit mapping and optogenetic activation

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   hChR2-YFP
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

   Plasmid

   #112496

   Purpose

   Expresses channel rhodopsin in astrocytes

   Depositor

   Baljit Khakh

   Article

   Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

   Insert

   ChR2(H134R)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   H134R

   Promoter

   GfaABC1D

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

   Plasmid

   #47905

   Purpose

   An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

   Plasmid

   #20293

   Purpose

   Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Alanine

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

   Plasmid

   #20295

   Purpose

   Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Threonine

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)

   Plasmid

   #166610

   Purpose

   Encodes Cre-inactivated ChR2-TdTomato under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   ChR2-TdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   H134R

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

   Plasmid

   #125256

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

   Depositor

   Roger D. Kamm

   Article

   Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

   Insert

   hChR2(H134R)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-forward

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

   Plasmid

   #37090

   Purpose

   Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   hChr2(H134R)-mCherry
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-DIO-hChR2(H134R)-P2A-EYFP

   Plasmid

   #139283

   Purpose

   Express hChR2(H134R)-P2A-EYFP in a Cre-dependent manner

   Depositor

   Mingshan Xue

   Article

   Chen et al Elife. 2020 Feb 19;9. pii: 48705. doi: 1

   Insert

   hChR2(H134R)-P2A-EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

    Plasmid

    #55648

    Purpose

    Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre off/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

    Plasmid

    #35510

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

    Plasmid

    #35503

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    Ef1a

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-nEF Con/Fon hChR2(H134R)-EYFP

    Plasmid

    #55644

    Purpose

    Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

    Plasmid

    #55645

    Purpose

    Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV5, AAV8, and AAV9

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hnEF Coff/Fon hChR2(H134R)-EYFP

    Plasmid

    #55649

    Purpose

    Cre-off/Flp-on ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre off/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Short Ef1

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP 2.0

    Plasmid

    #126081

    Purpose

    vCre-Dependent ChR2(H134R)-eYFP with the vLox sites in the reverse orientation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)-eYFP
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    H134R

    Promoter

    Ef1a

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

    Plasmid

    #55646

    Purpose

    Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp off chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid

    #201819

    Purpose

    A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    hChR2(H134R)-V5-ER-LBD (ChRERa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pOTTC1751

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

    Plasmid

    #35504

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-nEF Con/Foff hChR2(H134R)-EYFP

    Plasmid

    #55647

    Purpose

    Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp off chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Short Ef1a

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

    Plasmid

    #35509

    Purpose

    AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV5 and AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-nEF-Con/Foff 2.0-ChR2-EYFP

    Plasmid

    #137163

    Purpose

    Intersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-ChR2-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    H134R

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP

    Plasmid

    #47633

    Purpose

    An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    hChR2 (H134R)
    

    Use

    AAV and Cre/Lox

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    EF1a

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP

    Plasmid

    #137140

    Purpose

    Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-ChR2(ET/TC)-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E123T, T159C

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-DFO-ChR2(H134R)-EYFP-WPRE

    Plasmid

    #136916

    Purpose

    Expresses ChR2(H134R)-EYFP in Cre-negative cells

    Depositor

    Ofer Yizhar

    Article

    Sermet et al Elife. 2019 Dec 20;8. pii: 52665. doi: 1

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    H134R in ChR2

    Promoter

    Human Synapsin1

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-nEF-Con/Foff 2.0-ChR2-mCherry

    Plasmid

    #137143

    Purpose

    Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-ChR2-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    H134R

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

    Plasmid

    #50834

    Purpose

    An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.

    Depositor

    Brandon Harvey

    Article

    McDevitt et al Cell Rep. 2014 Sep 25;8(6):1857-1869. do

    Insert

    hChR2 (H134R)
    

    Use

    AAV and Cre/Lox

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    EF1a

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

    Plasmid

    #135176

    Purpose

    AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

    Depositor

    Tetsuo Yamamori

    Article

    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

    Inserts

    hChR2
    TRE3G
    

    Use

    AAV and Cre/Lox

    Tags

    EYFP (C terminal on insert)

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    TRE3G

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP 2.0

    Plasmid

    #126080

    Purpose

    sCre-dependent ChR2(H134R)-eYFP with the sLox sites in the reverse orientation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)-eYFP
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    H134R

    Promoter

    Ef1a

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid

    #201820

    Purpose

    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    hChR2(H134R)-V5-ER-LBD (ChRERa)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    SYN1

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

    Plasmid

    #118279

    Purpose

    Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

    Depositor

    Dietmar Schmitz

    Article

    Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

    Inserts

    mRuby2
    ChR2(H134R)
    

    Use

    AAV and Cre/Lox

    Tags

    SV40 NLS and YFP

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

    Plasmid

    #52878

    Purpose

    Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E90R, D156N, T159C

    Promoter

    CaMKIIa

    Available since

    July 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Vsx2 hR2-20 +SV40 base prom-GFP-IRES-AP

    Plasmid

    #225883

    Purpose

    Enhancer reporter

    Depositor

    Michael Dyer

    Insert

    hR2-20
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid

    #201822

    Purpose

    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    hChR2(H134R)-V5-ER-LBD (ChRERa)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKii

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HCT163_pAAV-eHGT_3Xcore_1137m-minBGprom-ChR2(H134R)-EYFP-WPRE3-bGHpA

    Plasmid

    #233562

    Purpose

    Direct-expressing ChR2(H134R)-EYFP AAV virus

    Depositor

    Tanya Daigle, The Allen Institute for Brain Science

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBglobin

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HCT164_pAAV-3X-eHGT_1139m-minBGprom-ChR2(H134R)-EYFP-WPRE3-bGHpA

    Plasmid

    #233563

    Purpose

    Direct-expressing ChR2(H134R)-EYFP AAV virus

    Depositor

    Tanya Daigle, The Allen Institute for Brain Science

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBglobin

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid

    #201821

    Purpose

    An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    hChR2(H134R)-V5-ER-LBD (ChRERa)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    SYN1

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid

    #20298

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid

    #20297

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid

    #65454

    Purpose

    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    H134R variant

    Promoter

    hSyn1

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid

    #65429

    Purpose

    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    H134R variant

    Promoter

    hSyn1

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AiP14870 - pAAV-AiE0452h_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN4870)

    Plasmid

    #224186

    Purpose

    AiE0452h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSNs

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. AiP13278: pAAV-AiE0779m_3xC2-minBG-ChR2(CRC)-EYFP-WPRE3-BGHpA (Alias: CN3278)

    Plasmid

    #214843

    Purpose

    Expression of ChR2(L132C, H134R, T159C)-EYFP in striatal direct pathway medium spiny neurons (D1 MSNs)

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    ChR2(L132C, H134R, T159C)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. AiP13276: pAAV-AiE0779m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3276)

    Plasmid

    #214842

    Purpose

    Expression of ChR2(H134R)-EYFP in striatal direct pathway medium spiny neurons (D1 MSNs)

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755)

    Plasmid

    #214846

    Purpose

    Expression of ChR2(H134R)-EYFP in striatal cholinergic neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. AiP14823: pAAV-AiE0441h_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN4823)

    Plasmid

    #214860

    Purpose

    Expression of ChR2(H134R)-EYFP in striatal medium spiny neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. AiP11763: pAAV-hEF1a-DIO-ChR2(CRC)-EYFP-WPRE-HGHpA (Alias: CN1763)

    Plasmid

    #236602

    Purpose

    A vector for Cre dependent expression of ChR2(CRC)-EYFP; CRC variant contains mutations L132C, H134R, and T159C in ChR2

    Depositor

    Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    ChR2(CRC)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. AiP12577 - pAAV-AiE0140h_3xC2-minBG-ChR2(CRC)-EYFP-WPRE3-BGHpA (Alias: CN2577)

    Plasmid

    #224166

    Purpose

    AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+ neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    ChR2(CRC)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188745

    Purpose

    Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    nonTATAchr21

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pDOC-K-glmS

    Plasmid

    #158058

    Purpose

    Donor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Inserts

    glmS homologous region 1
    glmS homologous region 2 + MCS3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge