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Showing: 2001 - 2050 of 157401 results

  12. pRL278

    Plasmid
    #70256
    Purpose
    sacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.
    Depositor
    Peter Wolk
    Article
    Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.
    Type
    Empty backbone
    Use
    Conditionally lethal
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70256-plasmid-map-sequence-id-125559

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  31. IgK-IFN-luc

    Plasmid
    #14886
    Purpose
    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression
    Depositor
    David Baltimore
    Article
    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.
    Insert
    IgK-IFN (IFNB1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2007
    Availability
    Academic Institutions and Nonprofits only
    14886-plasmid-map-sequence-id-238489

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  34. F2RL2-Tango

    Plasmid
    #66278
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    F2RL2 (F2RL2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  37. pJK2020

    Plasmid
    #171932
    Purpose
    express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon
    Depositor
    Judith Kimble
    Article
    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.
    Insert
    Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
    Use
    Tags
    superfolder GFP, mCherry
    Expression
    Worm
    Mutation
    Promoter
    -
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171932-plasmid-map-sequence-id-337179

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  40. TFORF2399

    Plasmid
    #144334
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZFPM1 (ZFPM1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    144334-plasmid-map-sequence-id-427297

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  43. (+)-Limonene Synthase

    Plasmid
    #112230
    Purpose
    (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.
    Depositor
    Daniel Oprian
    Article
    Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17
    Insert
    (+)-Limonene Synthase
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-52
    Promoter
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112230-plasmid-map-sequence-id-214310

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  44. pTXB1-TP5

    Plasmid
    #160088
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160088-plasmid-map-sequence-id-313736

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  47. pTXB1-TP3

    Plasmid
    #160086
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160086-plasmid-map-sequence-id-313733

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Showing: 2001 - 2050 of 157401 results