We narrowed to 16,884 results for: Por

1. lvl0-G-Gal4-VP64

   Plasmid

   #229186

   Purpose

   lvl 0 5 gene

   Depositor

   Ron Weiss

   Article

   Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

   Insert

   Gal4-VP64
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 6xABRE_A

   Plasmid

   #185803

   Purpose

   A synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the ABI1 promoter

   Depositor

   Jose Dinneny

   Article

   Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

   Insert

   6xABRE(ABI1)+-86MP+erGFP
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 6xABRE_R

   Plasmid

   #185804

   Purpose

   a synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the RD29A promoter

   Depositor

   Jose Dinneny

   Article

   Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

   Insert

   6xABRE(RD29A)+-86MP+erGFP
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACBE-ASR (pXK-607)

   Plasmid

   #208822

   Purpose

   Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.

   Depositor

   Kun Xu

   Article

   Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

   Type

   Empty backbone

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. XLone-mARG2

   Plasmid

   #173792

   Purpose

   Mammalian acoustic reporter gene 1 cassette 2 on XLone plasmid

   Depositor

   Xiaojun Lian

   Article

   Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

   Insert

   GvpN, F G L S K J U
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G promoter

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1_rSERT_X5C_S277C

   Plasmid

   #190177

   Purpose

   For mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibility

   Depositor

   Gary Rudnick

   Article

   Zhang et al J Biol Chem. 2006 Nov 24;281(47):36213-2

   Insert

   rat serotonin transporter (Slc6a4 Rat)
   

   Use

   Tags

   FLAG, His6, and c-myc

   Expression

   Mammalian

   Mutation

   Cys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…

   Promoter

   CMV

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEE22

   Plasmid

   #161787

   Purpose

   Hi-SC2 reporter delivery plasmid

   Depositor

   François-Xavier Barre

   Article

   Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

   Insert

   Hi-SC2 reporter
   

   Use

   Conjugative plasmid to deliver hi-sc2 reporter

   Tags

   Expression

   Mutation

   Himar site included MmeI site, tandem loxP sites,…

   Promoter

   n/a

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFGL1260

   Plasmid

   #116900

   Purpose

   eGFP:TubA (tubulin marker )

   Depositor

   Naweed Naqvi

   Article

   Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

   Inserts

   TubA promoter
   TubA (ORF+3'UTR)
   

   Use

   Fungal expression (in magnaporthe oryzae)

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFGL1260R

   Plasmid

   #116901

   Purpose

   mCherry:TubA (tubulin marker)

   Depositor

   Naweed Naqvi

   Article

   Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

   Inserts

   TubA promoter
   TubA (ORF+3'UTR)
   

   Use

   Fungal expression (in magnaporthe oryzae)

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNOC-stacked-GeNL-Ca

    Plasmid

    #101003

    Purpose

    Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

    Depositor

    Eva Farre

    Article

    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Fuw PBRM1 mBD4

    Plasmid

    #121108

    Purpose

    conditional expression

    Depositor

    Emily Dykhuizen

    Article

    Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

    Insert

    PBRM1 (N601A) (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PBRM1 (N601A)

    Promoter

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Fuw PBRM1 mBD5

    Plasmid

    #121109

    Purpose

    conditional expression

    Depositor

    Emily Dykhuizen

    Article

    Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

    Insert

    PBRM1 (N739A) (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PBRM1 (N739A)

    Promoter

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Fuw PBRM1 mBD1

    Plasmid

    #121105

    Purpose

    conditional expression

    Depositor

    Emily Dykhuizen

    Article

    Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

    Insert

    PBRM1 (Y127A) (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PBRM1 (Y127A)

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Fuw PBRM1 mBD3

    Plasmid

    #121107

    Purpose

    conditional expression

    Depositor

    Emily Dykhuizen

    Article

    Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

    Insert

    PBRM1 (N463A) (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PBRM1 (N463A)

    Promoter

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Fuw PBRM1 mBD2

    Plasmid

    #121106

    Purpose

    conditional expression

    Depositor

    Emily Dykhuizen

    Article

    Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

    Insert

    PBRM1 (N263A) (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PBRM1 (N263A)

    Promoter

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGS-3

    Plasmid

    #85094

    Purpose

    Host vector to fuse a gene at the N-terminus of PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC3

    Plasmid

    #85087

    Purpose

    Expresses PCNA3 protein fused to P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    PCNA3 fused to P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCVD404

    Plasmid

    #66149

    Purpose

    to make a probe to detect Heat stable enterotoxin gene (porcine)

    Depositor

    James Kaper

    Article

    Nishibuchi et al J Clin Microbiol. 1988 Apr;26(4):784-6.

    Insert

    STp
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pSIRV-NFAT-eGFP

    Plasmid

    #118031

    Purpose

    Fluorescent reporter for NFAT activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with NFAT binding site

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AMPK-SPARK

    Plasmid

    #226255

    Purpose

    phases-of-separation based activity reporter of AMPK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pSIRV-AP-1-mCherry

    Plasmid

    #118095

    Purpose

    Fluorescent reporter for AP-1 activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with AP-1 responsive element

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNMT

    Plasmid

    #42578

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    N-myristoyl tranferase (NMT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations T690C and T942C

    Promoter

    T7

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pSIRV-NF-kB-eCFP

    Plasmid

    #118094

    Purpose

    Flourescent reporter for NF-kB activity

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    eCFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with NF-kB responsive element

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PPRE-pNL1.3

    Plasmid

    #84394

    Purpose

    Secreted Luciferase reporter for PPARgamma activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

    Insert

    PPARG response element (PPARG Human)
    

    Use

    Tags

    secNluc

    Expression

    Mammalian

    Mutation

    Promoter

    PPRE

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PPRE-H2B-eGFP

    Plasmid

    #84393

    Purpose

    Fluorescent reporter for PPARgamma activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

    Insert

    PPARG response element (PPARG Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PPRE

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-1 and dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 8xNFAT-ZsG-mCD8

    Plasmid

    #162747

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCFD3-dU6:3gRNA

    Plasmid

    #49410

    Purpose

    expression of gRNA under control of the Drosophila U6:3 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-TRE-EGFP

    Plasmid

    #89875

    Purpose

    EGFP reporter vector of iTango system for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    tetO-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFH3.3_Cre-MS2x12

    Plasmid

    #205559

    Purpose

    Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. single cut NH-donor

    Plasmid

    #83575

    Purpose

    eGFP reporter donor containing single SA cutting site for NHEJ knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Nhej donor

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCSC-GFP-NES-EGFP

    Plasmid

    #221810

    Purpose

    Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    GFP-NES-EGFP
    

    Use

    Lentiviral

    Tags

    NES (internal tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. BFP

    Plasmid

    #201383

    Purpose

    Control nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Plasmid L5 attB::Pleft* mWasabi

    Plasmid

    #169409

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mWasabi
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Plasmid L5 attB::Pleft* mScarlet

    Plasmid

    #169410

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mScarlet proper
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLNCX2-CFP-VGPDFGR-YFP

    Plasmid

    #59587

    Purpose

    Encodes a granzyme B FRET reporter

    Depositor

    Tim Mitchison

    Article

    Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

    Insert

    ECFP-VGPDFGR-Venus
    

    Use

    Retroviral

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMuLE_ENTR_12GLI-FLuc_R4-R3

    Plasmid

    #113712

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Insert

    12GLI-FLuc
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GCaMP-AMPK-SPARK

    Plasmid

    #226257

    Purpose

    Dual reporter of Ca2+ levels and AMPK activity

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    GCaMP-AMPK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pRTC2-puro

    Plasmid

    #190567

    Purpose

    Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.

    Depositor

    Anthony Furano

    Article

    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    L1 (L1.3) with reporter cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUltra-5HTP

    Plasmid

    #178042

    Purpose

    Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    Modified ScTrpRS for 5HTP incoporation
    suppressor tRNA for 5HTP incorporation
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T107C, P254T, C255A

    Promoter

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. hSyn1-sypHy-RGECO

    Plasmid

    #84078

    Purpose

    Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

    Depositor

    Juan Burrone

    Article

    Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

    Insert

    sypHy-RGECO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEGFP-C1X-Nup93

    Plasmid

    #87336

    Purpose

    To express human Nup93 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP93 (NUP93 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMC-6-Ruby-9

    Plasmid

    #197740

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Ruby reporter tricistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. aCKAR

    Plasmid

    #124427

    Purpose

    FRET-based reporter for monitoring aPKC activity in cells

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

    Insert

    aCKAR
    

    Use

    Tags

    mCFP and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET30b_OmpAT7Sav

    Plasmid

    #138589

    Purpose

    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

    Insert

    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    

    Use

    Synthetic Biology

    Tags

    OmpA export signal and T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMuLE_ENTR_TOP-iRFP_L5-L4

    Plasmid

    #113866

    Purpose

    Single pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    TOP (wnt dependent promoter)
    iRFP713
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pHR iRFP-ZtSH2

    Plasmid

    #188627

    Purpose

    Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    ZtSH2
    

    Use

    Lentiviral

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. YFP-HA-DAT

    Plasmid

    #90244

    Purpose

    Expresses YFP-HA-DAT in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Sorkina et al J Neurosci. 2006 Aug 2;26(31):8195-205.

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    residues 193–203 of human DAT were replaced in YF…

    Promoter

    CMV

    Available Since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. Donor_eGP2AP_RC

    Plasmid

    #133784

    Purpose

    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

    Depositor

    Sriram Kosuri

    Article

    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

    Type

    Empty backbone

    Use

    Tags

    eGFP-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pSLHDR03_mCherry_tCD19_unique guide

    Plasmid

    #124062

    Purpose

    Construction of 1st donor for scarless genome editing with unique guide

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Inserts

    mCherry
    tCD19
    

    Use

    Mouse Targeting; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Human UbC modified

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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