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Plasmid#62970PurposeExpresses HA-tagged Gli1DepositorInsertGLI1 (GLI1 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cathepsin S S21C/C25A
Plasmid#84695Purposefor expression of mutant Cathepsin S in Leishmania tarantolaeDepositorInsertcathepsin S (CTSS Human)
UseExpression in leishmania tarantolaeTagshis-tagExpressionMutationS21C, C25APromoterunknownAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-14 (LA-14)
Plasmid#158750PurposeLentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.DepositorInsertLifeAct-14
UseLentiviralTagseGFPExpressionMammalianMutationDeletion mutation for last three amino acids from…PromoterCMV immediate earlyAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_fast
Plasmid#167271PurposeOverexpression of the fast variant (E30R) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
UseTagsHis x10ExpressionBacterialMutationE30RPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-CaMPARI V398D
Plasmid#60786PurposeExpresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integratorDepositorInsertCaMPARI V398D
UseTagsNuclear Export SignalExpressionMammalianMutationV398DPromoterCMVAvailable sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABEPuro-HA-GLI1
Plasmid#62967PurposeExpresses HA-tagged Gli1DepositorInsertGLI1 (GLI1 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A1
Plasmid#135503PurposeMammalian expression of myc-tagged HLA-A*01:01DepositorInsertHLA-A*01:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_AHR
Plasmid#101071PurposeDonor vector for 3' FLAG tag of human AHRDepositorInsertAHR homology arms (AHR Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Myc-CYLD-C601A
Plasmid#60027PurposeMyc-tagged CYLD catalytic inactive (C601A) expressionDepositorInsertCylindromatosis (CYLD Human)
UseTagsMycExpressionMammalianMutationCysteine 601 changed to AlaninePromoterCMVAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ3 SHP2 D61A
Plasmid#8320DepositorInsertSHP2 (PTPN11 Human)
UseTagsExpressionMammalianMutationD61A (activated mutant)PromoterAvailable sinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLPCX-VEcadTL
Plasmid#45849DepositorInsertVE-cadherin (Cdh5 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI1_G-alpha
Plasmid#110012PurposeProtein expression and purification of GNAI1_G-alphaDepositorInsertGNAI1_G-alpha (GNAI1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B8
Plasmid#135512PurposeMammalian expression of myc-tagged HLA-B*08:01DepositorInsertHLA-B*08:01 (HLA-B Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458_AHR_1
Plasmid#101076PurposeEncodes gRNA for 3' target of human AHRDepositorInsertgRNA against AHR (AHR Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-rnSOX9 PQA/S (286-507)
Plasmid#62975PurposeExpresses HA-tagged rat SOX9 residues 286-507DepositorInsertSOX9 (Sox9 Rat)
UseTagsHA (YPYDVPDY)ExpressionMammalianMutationcodes for residues 286-507PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTBNde(min)
Plasmid#15125DepositorInsertalpha-globin EDB fibronectin minigene (FN1 Human)
UseTagsExpressionMammalianMutationPromoteralpha-globinAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
Pvha6-AgDD
Plasmid#80626Purposecreate protein aggregates in C. elegansDepositorInsertNES-L106P-sfGFP (FKBP1A Human)
UseTagsMLALKLAGLDIExpressionWormMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
ppBAalbNixE1E2 PUb-GFP
Plasmid#173667Purposetransgenesis vector for Aedes albopictus ectopic expression of the Nix geneDepositorInsertsNix masculinisation gene
EGFP
UseTagsExpressionInsectMutationNix isoforms with only exons 1, 2 separated by in…PromoterAedes aegypti Polyubiquitin and cloned Nix promot…Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ EB3-mKate2
Plasmid#105940Purposein vitro transcriptionDepositorInsertEB3 (MAPRE3 Human)
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Dd
Plasmid#135518PurposeMammalian expression of myc-tagged H2-DdDepositorInsertH2-Dd (H2-D1 Mouse)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationC121SPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLin28A-myc-T2A-tagRFP
Plasmid#140267PurposeExpresses LIN28A in mammalian cellsDepositorInsertLIN28A (LIN28A Human)
UseSynthetic BiologyTagsmyc-T2A-tagRFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
CDK4-luciferase full length (2135)
Plasmid#86656PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation2120 base pairs including up to coding nucleotide…PromoterCDK4Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ3 SHP2 CS
Plasmid#8319DepositorInsertSHP2 (PTPN11 Human)
UseTagsExpressionMammalianMutationC459S, mutation of essential catalytic cysteinePromoterAvailable sinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
PX458_AHR_2
Plasmid#101077PurposeEncodes gRNA for 3' target of human AHRDepositorInsertgRNA against AHR (AHR Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_SCP1_empty
Plasmid#99299PurposeLuciferase validation vector; empty vector without insert to determine baselineDepositorTypeEmpty backboneUseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
GA-DEVD-GB-NES
Plasmid#61020Purposegreen ddFP based caspase-3 biosensorDepositorInsertddGFP A and ddGFP B
UseTagsHindIII and NES sequence LALKLAGLDIGS placed afte…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p37)
Plasmid#135939PurposeExpresses AUF1p37 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Rhodoplanes-bGSDM
Plasmid#212129PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRhodoplanes bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM
Plasmid#212124PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertBacteroidetes bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Runella-bGSDM
Plasmid#212123PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRunella bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Vitiosangium-bGSDM
Plasmid#212127PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertVitiosangium bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Gillisia-bGSDM
Plasmid#212128PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertGillisia bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-BNT162b2ΔUTR
Plasmid#171214PurposeGateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.DepositorInsertBNT162b2ΔUTR
UseGateway cloningTagsExpressionMutationKozak sequence changed from CCCGCCACC to GGCTGCAC…PromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p45)
Plasmid#135942PurposeExpresses AUF1p45 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
HS-EB3-GFP
Plasmid#105948Purposeheat shock inducible transgenesisDepositorInsertEB3 (MAPRE3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-FLAG-CYLD-WT
Plasmid#60030PurposeWT CYLD under control of Tet promoter with N-terminal FLAG tagDepositorInsertCylindromatosis (CYLD Human)
UseTagsFLAGExpressionMammalianMutationPromoterTREAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi1-91-NLuc
Plasmid#207148PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI1 (GNAI1 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_UGControl
Plasmid#133875PurposeExpresses UGControl in mammalian cellsDepositorInsertUtrCH (UTRN Human)
UseTagsflexible linker - mEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)
Plasmid#167268PurposeOverexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coliDepositorInsertHOB
UseTagsHis x10ExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only