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pMRE-Tn7-140

Plasmid

#118556

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PP7 guide

Plasmid

#86168

Purpose

Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

PP7 guide

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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T7-pAc5.1 mCherry EcoRV

Plasmid

#206490

Purpose

Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Type

Empty backbone

Tags

mCherry

Expression

Insect

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDM042

Plasmid

#216812

Purpose

Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mScarlet3

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-hAtg101

Plasmid

#22877

Depositor

Noboru Mizushima

Article

Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

Insert

Atg13-interacting protein (ATG101 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only
pRK5-HA-UFM1-dCS

Plasmid

#139869

Purpose

Mammalian expression of HA-tagged human UFM1-cCS

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFM1 (UFM1 Human)

Tags

HA

Expression

Mammalian

Mutation

d84-85

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHEE-R-hyg9si

Plasmid

#222832

Purpose

CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMS77

Plasmid

#215681

Purpose

Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-DEVD-mCardinal-NLS

Plasmid

#164052

Purpose

Fluorescent reporter for caspase-3

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-mCardinal-NLS

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-p2A-full length LIC1

Plasmid

#74601

Purpose

expresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherry

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 (DYNC1LI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLI

Plasmid

#131228

Purpose

Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Coding sequence has been codon optimised for expr…

Promoter

CMV enhancer + CMV promoter

Available Since

Dec. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-Ef1a-SOX2-3xFLAG

Plasmid

#216197

Purpose

Overexpress human SOX2 in HEK 293T cells for use in whole cell extract EMSAs

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX2 (SOX2 Human)

Use

Lentiviral

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-146

Plasmid

#118562

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Fon/ConN2cG

Plasmid

#234709

Purpose

pAAV transfer plasmid with Ef1a promoter driving Cre- and Flp-dependent expression of G protein for rabies N2c delta G

Depositor

David Ng

Article

Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

Insert

Fon Con N2cG

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pB-3xFlag-dCas13-mTET2CD

Plasmid

#228234

Purpose

For targeted tethering of the catalytic domain of mouse TET2 using dCas13

Depositor

Chuan He

Article

Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

Inserts

dCas13
TET2 (Tet2 Mouse)

Use

CRISPR

Expression

Mammalian

Mutation

CD (cysteine-rich, dioxygenase domain) truncation…

Promoter

CAG

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1A-WT-Flag-UTR

Plasmid

#183237

Purpose

Expression of FLAG tagged HNF1A

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox A (HNF1A Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAGT9050

Plasmid

#219429

Purpose

PapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)

Depositor

Tom Schreiber

Article

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

AATG_PapE-4xLF2_AGGT

Use

Moclo compatible level 0 module

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-BFP

Plasmid

#153426

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

mTagBFP2

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19_CoV-2_linker

Plasmid

#211731

Purpose

Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Linker fragment SARS-CoV-2

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)

Plasmid

#65446

Purpose

Expression of ephrinB1 and RFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

ephrin B1 (Efnb1 Mouse)

Use

Lentiviral

Tags

SV40-RFP

Expression

Mammalian

Promoter

UBQ

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only