176,417 results

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pEGFP-parkin WT

Plasmid

#45875

Depositor

Edward Fon

Publication

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pscAAV-GFP

Plasmid

#32396

Depositor

John T Gray

Publication

Gray et al Methods Mol Biol. 2011;807:25-46.

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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pLX303

Plasmid

#25897

Purpose

3rd generation lentiviral Gateway destination vector. Blasticidin selection.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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phOCT4-Luc

Plasmid

#17221

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Upstream region of human OCT4 (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCMV5B-Flag-Smad3

Plasmid

#11742

Depositor

Jeff Wrana

Publication

Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

Insert

SMAD3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Available since

Aug. 7, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

16 Articles

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pCMV-PE1

Plasmid

#132774

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Publication

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE1

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGP-CMV-jGCaMP7s

Plasmid

#104463

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7s

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Publication

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Expression

Mammalian

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pET-15b-EK_C122S_His5

Plasmid

#49048

Purpose

Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

Depositor

Hans Brandstetter

Publication

Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

Insert

Enteropeptidase, peptidase domain (TMPRSS15 Bovine)

Tags

5xHis

Expression

Bacterial

Mutation

P92R, E185D, C122S

Promoter

T7

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mCh-Rab5

Plasmid

#49201

Purpose

expression of Rab5 fused to mCherry

Depositor

Gia Voeltz

Publication

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Insert

Rab5 (RAB5B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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5HRE/GFP

Plasmid

#46926

Purpose

hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

Depositor

Martin Brown, Thomas Foster

Publication

Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

Insert

5X HRE of VEGF (VEGFA Human)

Tags

d2EGFP

Expression

Mammalian

Mutation

five copies of a 35-bp fragment from the hypoxia-…

Promoter

EF-1α promoter

Available since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pRS300

Plasmid

#22846

Purpose

Backbone for expressing plant artificial miRNAs

Depositor

Detlef Weigel

Publication

Schwab et al Plant Cell. 2006 May . 18(5):1121-33.

Insert

MIR319a (MIR319a Mustard Weed)

Use

RNAi; Plant gene inactivation

Expression

Plant

Available since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCAGGS-flpE-puro

Plasmid

#20733

Purpose

Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

Depositor

Rudolf Jaenisch

Publication

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

FLPe recombinase

Use

Flp/frt

Expression

Mammalian

Promoter

CAGGS

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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lenti-EF1a-dCas9-KRAB-Puro

Plasmid

#99372

Purpose

3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-KRAB-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pdCas9-DNMT3A-EGFP

Plasmid

#71666

Purpose

Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Publication

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

CBh

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pMK289 (mAID-mClover-NeoR)

Plasmid

#72827

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Neo

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pInducer10-mir-RUP-PheS

Plasmid

#44011

Purpose

Lentiviral shRNA vector (pGIPz based) for inducible knockdown

Depositor

Stephen Elledge

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Promoter

TRE2

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pmTurquoise2-ER

Plasmid

#36204

Purpose

In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

ER-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pBiFC-bFosVC155

Plasmid

#22013

Depositor

Chang-Deng Hu

Publication

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bFosVC155

Tags

HA

Expression

Mammalian

Mutation

N/A

Available since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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RelA cFlag pcDNA3

Plasmid

#20012

Depositor

Stephen Smale

Publication

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

RelA (Rela Mouse)

Tags

Flag

Expression

Mammalian

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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