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Plasmid#127332PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRESDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-Puro
Plasmid#141393PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf9c
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro
Plasmid#141381PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp15 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-Puro
Plasmid#141394PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf10 (ORF10 SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro
Plasmid#141389PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf7b (ORF7b SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR- GAL4-UAS-BAMH1-IRES-mCherry-PGK-BFP
Plasmid#223215PurposeLentiviral vector - mCherry reporter with a BAMH1 site for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertmCherry BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro
Plasmid#141377PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp11 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1
Plasmid#138417PurposeCAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1DepositorInsertsBirA
dCas9
UseLentiviralTagsFLAG, Avi-tag and V5, HisExpressionMutationPromoterEF1aAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP
Plasmid#223213PurposeLentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertTGFb1 active - membrane (TGFB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE mNeonGreen-Core (HBc from HBV) IRES puro
Plasmid#122202PurposePlasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.DepositorInsertsHBc from HBV AYW
mNeonGreen
UseTagsGGSGGSGGS linkerExpressionMammalianMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo
Plasmid#222636PurposeEzrin activation reporterDepositorInsertmiRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
UseLentiviralTagsmRuby3ExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1
Plasmid#184397PurposeDonor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1DepositorInsertFirefly luciferase beta-globin-PTC39
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPTC in beta-globin at amino acid position 39PromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO
Plasmid#52419PurposeConstitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.DepositorInsertERAS (ERAS Human)
UseTagsExpressionMutationPromoterCAGGSAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP
Plasmid#32481PurposeCre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertPSAML141F,Y115F-GlyR
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-UAS-huCD25- IRES-mCherry-PGK-BFP
Plasmid#223211PurposeLentiviral vector - mCherry reporter and huCD25 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertCD25 (IL2RA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Stop-NPM-ALK-MSNA-IRES-EGFP (MSNAIE)
Plasmid#154470PurposecDNA of NPM-ALK in the MSE backboneDepositorInsertNPM-ALK
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS
Plasmid#191369PurposeOverexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cellsDepositorInsertNLS-NLS-dPba3Cas13b-3xGFP-NLS
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_UCOE-EF1a-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry
Plasmid#188769PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS
Plasmid#191368PurposeOverexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cellsDepositorInsertNLS-NLS-dHgm4Cas13b-3xGFP-NLS
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S
Plasmid#62458Purposeexpresses his-tagged C151S human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionMammalianMutationChanged Cysteine 151 of Keap1 to serinePromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69546PurposeTet-On system expressing MyoD-2A-GFP-2xNLS.DepositorInsertsUseLentiviralTags2x nuclear localization sequence and T2A "sk…ExpressionMutationRemoved stop codon to fuse it to the 2A-eGFPnls i…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag
Plasmid#132412Purposeoverexpression in human cellsDepositorInsertdAdmCas13b
UseLentiviralTagsflagExpressionMammalianMutationR269A; H274A; R837A; H842APromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12
Plasmid#101285PurposeLentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFPDepositorInsertE-Cadherin-GFP-H-Ras
UseLentiviralTagsGFPExpressionMammalianMutationH-Ras V12PromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S
Plasmid#62459Purposeexpresses his-tagged C288S human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionMammalianMutationChanged Cysteine 288 of Keap1 to serinePromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1
Plasmid#183402PurposeDual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-FKBP-SMBiT-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag
Plasmid#132410Purposeoverexpression in human cellsDepositorInsertdEsCas13b
UseLentiviralTagsflagExpressionMammalianMutationR295A; H300A; R849A; H854APromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171595PurposeWeak human ACE2 abundance from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1
Plasmid#183400PurposeDual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
Plasmid#40571DepositorInsertAurora B FRET-sensor N-terminally fused to histone 2B
UseTagsCyPet and YPetExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag
Plasmid#132409Purposeoverexpression in human cellsDepositorInsertdRanCas13b
UseLentiviralTagsflagExpressionMammalianMutationH147A; H1044APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry
Plasmid#84258PurposeExpresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gateDepositorInsertsSp sgTRE3G
PYL1-VPR
UseCRISPR and LentiviralTagsIRES-mCherry and PYL1ExpressionMammalianMutationPromoterCMV and mouse U6Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry
Plasmid#114726PurposedCas9-VP160 expression in mammalian cellsDepositorInsertdCas9-VP160
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry
Plasmid#84260PurposeExpresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulationDepositorInsertsSp sgSV40
PYL1-KRAB
UseCRISPR and LentiviralTagsIRES-mCherry and PYL1ExpressionMammalianMutationPromoterCMV and mouse U6Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro
Plasmid#61441PurposeDox-inducible MyoD with mRNA tag for smFISHDepositorInsertMyoD (Myod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR
Plasmid#75136PurposeRetroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9)DepositorInsertshBrd9_1116
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR
Plasmid#75135PurposeRetroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9)DepositorInsertshBrd9_1061
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag
Plasmid#185683PurposeLow-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Synthetic, Human)
UseTagsHaloTagExpressionMammalianMutation(WLLI-GSGS)[359-362]PromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry
Plasmid#188770PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49218PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron
Plasmid#235087PurposeGFP splicing reporter with FRG1 intronDepositorInsertBFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)
UseAAVTagsExpressionMammalianMutationPromotercustom bidirectionalAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron
Plasmid#235086PurposeGFP splicing reporter with CORO1B intronDepositorInsertBFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)
UseAAVTagsExpressionMammalianMutationPromotercustom bidirectionalAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vsx2 mR0-37a +SV40 base prom-GFP-IRES-AP
Plasmid#225889PurposeEnhancer reporterDepositorInsertmR0-37a
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vsx2 mR3-17c +SV40 base prom-GFP-IRES-AP
Plasmid#225890PurposeEnhancer reporterDepositorInsertmR3-17c
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vsx2 mR3-17d +SV40 base prom-GFP-IRES-AP
Plasmid#225891PurposeEnhancer reporterDepositorInsertmR3-17d
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vsx2 Mini-enhancer +SV40 base prom-GFP-IRES-AP
Plasmid#225893PurposeEnhancer reporterDepositorInsertVSX2 Mini-enhancer
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
Plasmid#233441PurposePiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR)DepositorInsertCCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A
Plasmid#223560PurposeAlias: Gαi bONE-GO W139A. Lentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotorDepositorInsertYFP(Venus) - KRas4b - IRES* - human GINIP W139A - linker(GGGS) - Nluc
UseLentiviralTagsExpressionMammalianMutationW139A mutation in GINIP sequencePromoterCMVAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP1200 - pAAV-AiE2010m-minBG-IRES2-iCre-WPRE-HGHpA
Plasmid#230586PurposeAiE2010m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertiCre
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SNAP-p53DD-IRES-HRAS(G12V)-bGH
Plasmid#233204PurposeAAV expression of SNAP-p53DD and HRAS(G12V)DepositorUseAAVTagsExpressionMammalianMutationG12VPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry
Plasmid#232244PurposeMammalian expression of N581K RLIM-GFP-IRES-mCherryDepositorInsertN581K RLIM-GFP-IRES-mCherry (RLIM Human)
UseTagsAcGFP1ExpressionMammalianMutationN581KPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only