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We narrowed to 5,876 results for: nls

Showing: 2501 - 2550 of 5876 results

  9. pT2iApowriter2(#336)

    Plasmid
    #184104
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis
    Tags
    internal tag: 5myc
    Expression
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184104-plasmid-map-sequence-id-364017

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  10. pT2iU6Apowriter2N(#355)

    Plasmid
    #184106
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis + in vitro trans…
    Tags
    internal tag: 5myc
    Expression
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi+SP6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184106-plasmid-map-sequence-id-364015

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  11. pT22Apowriter2(#340)

    Plasmid
    #184105
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    internal tag: 5myc
    Expression
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184105-plasmid-map-sequence-id-363997

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  12. pT22iuasvApoWChe(#488)

    Plasmid
    #184108
    Purpose
    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    Expression
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    4xUAS
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184108-plasmid-map-sequence-id-363970

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  13. Lenti-BAT-RED

    Plasmid
    #20674
    Depositor
    Tannishtha Reya
    Insert
    Wnt reporter dsRED
    Use
    Tags
    NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 26, 2009
    Availability
    Academic Institutions and Nonprofits only

  14. pMP416

    Plasmid
    #134996
    Purpose
    dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    Use
    Synthetic Biology
    Tags
    3xFLAG, E coli Dam(N132A), and dCas9
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134996-plasmid-map-sequence-id-267931

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  15. pET21a-Streptavidin-Alive

    Plasmid
    #20860
    Purpose
    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Alive
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Wt gene
    Promoter
    Available since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20860-plasmid-map-sequence-id-194024

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  16. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Promoter
    Available since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

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  19. pTriEx4-Cdc42-2G

    Plasmid
    #68814
    Purpose
    FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)
    Depositor
    Olivier Pertz
    Article
    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10
    Insert
    Cdc42 activation reporter
    Use
    Tags
    His and Myc
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    68814-plasmid-map-sequence-id-120972

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  31. pStr-KDEL-IRES-ST-mCherry

    Plasmid
    #110727
    Purpose
    Expresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherry
    Depositor
    David Stephens
    Article
    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180
    Inserts
    Str-KDEL
    STase sialyltransferase (ST6GAL1 Human)
    Use
    Tags
    Str Streptavidin binding peptide (SBP) and mCherr…
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 43 of ST
    Promoter
    CMV and IRES
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    110727-plasmid-map-sequence-id-259745

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  35. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51055-plasmid-map-sequence-id-77327

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  39. JAB3494

    Plasmid
    #190418
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter )
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    190418-plasmid-map-sequence-id-393672

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  40. JAB2602

    Plasmid
    #190389
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    190389-plasmid-map-sequence-id-393424

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  43. pRvACT-mCherry

    Plasmid
    #205002
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205002-plasmid-map-sequence-id-405174

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  48. JAB2603

    Plasmid
    #190390
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    190390-plasmid-map-sequence-id-393426

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Showing: 2501 - 2550 of 5876 results