We narrowed to 6,273 results for: nls
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Plasmid#66599PurposeExpresses WDR34 as a fusion with monomeric mRubyDepositorAvailable SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only
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Sus1/PET32a
Plasmid#26963DepositorInsertSus1 (SUS1 Budding Yeast)
TagsHis6 and TrxExpressionBacterialMutationwith TEV site to cleave the N-terminal his+Trx tagAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCHW00015
Plasmid#222446PurposeLevel 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.DepositorInsertVP64-NLS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW00014
Plasmid#222445PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.DepositorInsertVP16-NLS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD
Plasmid#216396PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1DepositorInsertEfg1-ΔDBD
Tags6xHis-tagsExpressionBacterialMutationdeleted amino acids 182-356Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CMV-SCLM
Plasmid#216758PurposeAAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleonDepositorInsertSuperClomeleon followed by WPRE and human growth hormone polyA terminator
UseAAVExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCMV (cytomegalovirus)Available SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ300
Plasmid#205414PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoterDepositorInsertEv1Cas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLS (N terminal on insert) NLS (C terminal on ins…ExpressionPlantAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3477
Plasmid#190416PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (6XPhlFO-3XAmtRO::TetR-NLS-19St)DepositorInsert6XPhlFO-3XAmtRO::TetR-NLS-19St
ExpressionPlantAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3617
Plasmid#190427PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (pro35S::TetR-NLS-19St)DepositorInsertpro35S::TetR-NLS-19St
ExpressionPlantAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3017
Plasmid#190399PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::McbR-NLS-19St)DepositorInsertproG1090::McbR-NLS-19St
ExpressionPlantAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2964
Plasmid#190398PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (6XAmtRO-2XPhlFO::TetR-NLS-19St)DepositorInsert6XAmtRO-2XPhlFO::TetR-NLS-19St
ExpressionPlantAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC0_3_Mxi1
Plasmid#163715PurposePlasmid encoding Mxi1 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)DepositorInsertMxi1+SV40 NLS
UseSynthetic Biology; Moclo vector level 0 (type 4a)Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Himar-dCas9-mammalian
Plasmid#138414PurposeProtein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tagDepositorInsertHimar-dCas9
Tags3xFLAG, 6xHis, and SV40 NLSExpressionBacterialPromoterT7Available SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM767
Plasmid#133604PurposePart Plasmid for dCas9 NLS, Part 3(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM768
Plasmid#133605PurposePart Plasmid for dCas9 NLS, Part 3b(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM769
Plasmid#133606PurposePart Plasmid for dCas9 NLS, Part 3a(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Intersectin SH3 domain A
Plasmid#47396DepositorInsertIntersectin SH3 domain A
TagsGSTExpressionBacterialMutationSH3 domain APromotertacAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH127
Plasmid#162483PurposeSEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertmyo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsmyo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH130
Plasmid#162484PurposeSEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertmyo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsmyo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB–DualPE
Plasmid#235161PurposeFor plant prime editing including large DNA fragment editing in wheat plants or other monocotyledonsDepositorInsertsCsy4-P2A-Nls-nCas9-NC-nls-MLV-Nls
CmYLCV-epegRNA-CaMV poly(A) signal
UseCRISPRMutationDetailed in manuscriptAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pErCas12a EZ Clone
Plasmid#132641PurposeExpresses ErCas12a and an sgRNA to target a region of interestDepositorInsertErCas12a
UseAAV and CRISPRTags3x HA and SV40 NLSExpressionMammalianMutationQ926R (Please see depositor comments) and BsaI si…PromoterCMV PromoterAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH126
Plasmid#162482PurposeSEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertvha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsvha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-SpCas9
Plasmid#130968Purpose3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-SpCas9
UseCRISPRTags3x HA, EGFP, and NLSExpressionMammalianPromoterCbhAvailable SinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
enOgeuIscB with ωRNA-v2
Plasmid#220939PurposeCMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloingDepositorInsertsCMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
UseCRISPRExpressionMammalianAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJ915
Plasmid#69090PurposeExpress Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLSDepositorInsertcas9
TagsMBP, hexa His, and two SV40 NLSExpressionBacterialPromoterT7Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCasRx-RBM25
Plasmid#221001PurposeTransient expression of dCasRx-RBM25. EF1a promoter.DepositorInsertdCasRx-RBM25
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterEF1aAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH-MYC
Plasmid#101765PurposeGateway-compatible destination vector for mammalian expression of N-terminal Myc-tag fusion proteinDepositorTypeEmpty backboneTagsMycExpressionMammalianAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (PX165)
Plasmid#48137PurposeHuman codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)DepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMH-HA
Plasmid#101767PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion proteinDepositorTypeEmpty backboneTagsHAExpressionMammalianAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pprm1-luciferase; his-3
Plasmid#137728PurposeCommunication-activated luciferase in Neurospora crassaDepositorInsertsLuciferase
Pprm-1 Promoter
UseLuciferase; Neurospora crassa expressionMutationCodon-optimized for N. crassaAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
p4455 FLAG-hPLIC-2
Plasmid#8661DepositorAvailable SinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCR1265
Plasmid#111094Purposelentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1DepositorTypeEmpty backboneUseLentiviralAvailable SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
SepOTSλ
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE1
Plasmid#73019PurposeExpresses BE1 with C-terminal NLS in mammalian cellsDepositorInsertBE1
ExpressionMammalianPromoterCMVAvailable SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC1802_pmax-RBFOX1N-dCasRx-C
Plasmid#118635PurposeTransient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoterDepositorInsertRBFOX1N-dCasRx-C
UseCRISPRExpressionMammalianPromoterCAGGSAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
enOgeuIscB with ωRNA-v13
Plasmid#220940PurposeCMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloingDepositorInsertsCMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
UseCRISPRExpressionMammalianAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE-EGFP
Plasmid#89871PurposeEGFP reporter vector for iTango systemDepositorInserttetO-EGFP
ExpressionMammalianPromoterTetOAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
PSD95-TS:YFP
Plasmid#42225DepositorInsertPSD95
TagsTS:YFPExpressionMammalianAvailable SinceFeb. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
p4458 FLAG-hPLIC-1
Plasmid#8663DepositorAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMH-MBP
Plasmid#101768PurposeGateway-compatible destination vector for bacterial expression of N-terminal MBP-tag fusion proteinDepositorTypeEmpty backboneTagsMBPExpressionBacterialAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
SupD v2
Plasmid#198598PurposePlasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.DepositorInsertSupD
ExpressionBacterialAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAT15489_dABE8e
Plasmid#174127Purposeplasmid expressing ABE8e with dCas9DepositorInsertdABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZ149-pcDNA3-BhCas12b_v4
Plasmid#122446PurposeExpresses BhCas12b_v4 in mammalian cellsDepositorInsertCMV-NLS-BhCas12b_v4-NLS-3xHA
ExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAT15482_ABE8e
Plasmid#174120PurposeExpresses ABE8e with an EF1a promoterDepositorInserteABE
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
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p1195-pssAAV.U1a.hNme2Cas9
Plasmid#129534PurposeAAV vector expressing Nme2Cas9DepositorInserthuman codon-optimized Nme2Cas9
UseAAVTags2xNLS and NLS-3xHA-NLSPromoterU1aAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ143-pcDNA3-BvCas12b
Plasmid#122445PurposeExpresses BvCas12b in mammalian cellsDepositorInsertCMV-NLS-BvCas12b-NLS-3xHA
Tags3XHAExpressionMammalianAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
XW52
Plasmid#65838PurposeXW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional methodDepositorInsertCzipper-QF-AD-NLS
TagsmCherryExpressionWormPromoterPmig-13Available SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB62
Plasmid#47944PurposeExpresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormPromotereef-1A.1 (eft-3)Available SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only