We narrowed to 5,415 results for: PEP
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Plasmid#109797PurposeProtein expression and purification of DOK5_IRSDepositorInsertDOK5_IRS (DOK5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOK1_IRS
Plasmid#109779PurposeProtein expression and purification of DOK1_IRSDepositorInsertDOK1_IRS (DOK1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
eNS2B47NS3 S135A
Plasmid#155318PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationS135APromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(C104A/M247C)
Plasmid#155133PurposeExpresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationC104A/M247C, insert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-Dronpa
Plasmid#138250PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-Dronpa
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
D19_Ld_130940
Plasmid#135835PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertsubtilisin-like serine peptidase,serine peptidase, clan SB, family S8-like protein
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts2
Plasmid#132714PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat NtsDepositorInsertshNts(2) (Nts Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts1
Plasmid#132713PurposeEncodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts geneDepositorInsertshNts(1) (Nts Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DEPTOR_DEP
Plasmid#109882PurposeProtein expression and purification of DEPTOR_DEPDepositorInsertDEPTOR_DEP (DEPTOR Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(WT)
Plasmid#112720PurposeWild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.DepositorInsert6His-TEV-TBP6.7(WT)
UseTags6His-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
UseTags6His-TEVExpressionBacterialMutationP46APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
UseTags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
UseTags6His-TEVExpressionBacterialMutationP51APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106461PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106470PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMflCT-o4
Plasmid#101313PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o3
Plasmid#101311PurposeContains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.DepositorInsertoriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS118 His14-Avi-28xGS-anti ALFA tag nanobody
Plasmid#199371PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-ALFA nanobody
UseTags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV-sct-pp65-b2m-HLA-A0201
Plasmid#196511PurposeExpress single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)DepositorInsertsingle chain of a pp65 peptide, b2m and HLA A0201
UseLentiviralTagsNAExpressionMammalianMutationY84CPromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisCoTune
Plasmid#173713PurposePromotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.DepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI2_G-alpha
Plasmid#109791PurposeProtein expression and purification of GNAI2_G-alphaDepositorInsertGNAI2_G-alpha (GNAI2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI3_G-alpha
Plasmid#110014PurposeProtein expression and purification of GNAI3_G-alphaDepositorInsertGNAI3_G-alpha (GNAI3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-XBP1s
Plasmid#63680PurposeMammalian expression of Flag tagged XBP1sDepositorInsertXBP1 (XBP1 Human)
UseTagsFlagExpressionMammalianMutationsplicedPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOTTC407 - pAAV EF1a eGFP
Plasmid#60058PurposeAn AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.DepositorInsertenhanced Green Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-Flag-XBP1u
Plasmid#63679PurposeAdenoviral shuttle vector for XBP1u expressionDepositorInsertXBP1 (XBP1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationunsplicedPromoterCMVAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMT3_SRSF1
Plasmid#201056PurposeExpress sumo-tagged human SRSF1DepositorInsertSRSF1 (SRSF1 Human)
UseTagssumoExpressionBacterialMutationnonePromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
OPEN HLA-A*02:01-BirA
Plasmid#215569PurposeE. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.DepositorInsertOPEN HLA-A*02:01 (HLA-A Human)
UseTagsBirAExpressionBacterialMutationGlycine 120 to CysteinePromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-tagRFP-KDEL
Plasmid#114177PurposeGateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ERDepositorInserttagRFP-KDEL
UseGateway entry cloneTagsKDEL retention signal and signal peptideExpressionMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
OPEN-HLA-A*11:01-BirA
Plasmid#235135PurposeE. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.DepositorInsertOPEN-HLA-A*11:01 (HLA-A Human)
UseTagsBirAExpressionBacterialMutationG120CPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
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eNS2B47NS3 L30SF31S
Plasmid#155319PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
OTR untagged
Plasmid#208913Purposeencodes the human oxytocin receptor, without any tagsDepositorInsertOXTR (OXTR Human)
UseTagsExpressionMammalianMutationTM5: 5x56, A218, Threonin instead of AlaninPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DNAseI
Plasmid#182409PurposeBacterial expression of Vesicle Nucleating peptide-DNaseI fusionDepositorInsertVNp-DNase I (DNASE1 Bovine)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T7-6h-GST-α-Synuclein
Plasmid#225224PurposeAlpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.DepositorInsertsGST
α-Synuclein
UseTags6xHis Tag and TEV Cleavage SiteExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)
Plasmid#199605PurposeExpression of cell permeable opAsCas12a in E. Coli.DepositorInsertopCas12a-T8N
UseCRISPRTags6xHis, Twin-strep, SUMOExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Large BiT - SNAP-tag - Beta-actin
Plasmid#171019PurposeLentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cellsDepositorInsertLargeBit-SNAPtag- Beta-actin
UseLentiviral and LuciferaseTagsBeta actin and LargeBiTExpressionMammalianMutationPromoterEF1Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Etanercept
Plasmid#182421PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusionDepositorInsertVNp-mNeongreen-Etanercept (TNFRSF1B Human)
UseTagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
A*66:01:01
Plasmid#203291PurposeOverexpress HLA alleleDepositorInsertA*66:01:01 (HLA-A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1_T6B -mCherry
Plasmid#205600Purposeexpresses human T6B peptide fused with mChery in mammalian cellsDepositorInsertT6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
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A*11:01:01:01
Plasmid#203276PurposeOverexpress HLA alleleDepositorInsertA*11:01:01:01 (HLA-A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-DYRK1A
Plasmid#101770PurposeMammalian expression of DYRK1A fusion proteinDepositorInsertDYRK1A (DYRK1A Human)
UseTagsS peptide-flag-streptavidin binding peptide (SFB)ExpressionMammalianMutationsiRNA-resistant allelePromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Zeo_ARRB2-1-383-CTEV-2xHA
Plasmid#194382PurposeSplit TEV assaysDepositorInsertARRB2-CTEV-2xHA (ARRB2 Human, Synthetic)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRB2_SH3
Plasmid#110015PurposeProtein expression and purification of GRB2_SH3DepositorInsertGRB2_SH3 (GRB2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-StefinA
Plasmid#182419PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusionDepositorInsertVNp-mNeongreen-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-H
Plasmid#223966PurposeAnti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRM055 His-TEV-Cas9 6xNLS 0C
Plasmid#196246PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only