We narrowed to 3,123 results for: BID

1. pPHR-EYFP-hPMLIII

   Plasmid

   #103825

   Purpose

   Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-hPMLIII (CRY2, PML Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. STARR-seq luciferase validation vector_ORI_IGF1R

   Plasmid

   #99306

   Purpose

   Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr15: 99439352 -99440791
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIBN-TetR-tagRFP-T

   Plasmid

   #103809

   Purpose

   'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-TetR-tagRFP-T (CIB1, tetR Mustard Weed, E. coli)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIBN-EYFP-TetR

   Plasmid

   #103798

   Purpose

   BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-TetR (CIB1, tetR Mustard Weed, E. coli)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1mLCAD_K42R_FLAG

   Plasmid

   #24486

   Depositor

   Eric Verdin

   Article

   Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

   Insert

   Long chain acyl-CoA dehydrogenase (Acadl Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lys42Arg

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pTKan-p35S::Csy4-pNOS::cogRFP (C134, JBEI-15901)

   Plasmid

   #110146

   Purpose

   Transformation and expression of Csy4 and cogDsRed proteins in plants

   Depositor

   Dominique Loque

   Article

   Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

   Insert

   Csy4 (ATCS Mustard Weed)
   

   Use

   Tags

   cogRFP

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. STARR-seq luciferase validation vector_SCP1_CMV

   Plasmid

   #99314

   Purpose

   Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   CMV enhancer
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

   Plasmid

   #139482

   Purpose

   Expresses PhyB621-mCherry-HRasCT in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   PhyB621 (PHYB Mustard Weed)
   

   Use

   Lentiviral

   Tags

   mCherry-HRasCT

   Expression

   Mammalian

   Mutation

   G229L and deletion of aa Y at position 235 in mch…

   Promoter

   Tet responsible element

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)

   Plasmid

   #110145

   Purpose

   Transformation and expression of Csy4 and cogGFP proteins in plants

   Depositor

   Dominique Loque

   Article

   Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

   Insert

   Csy4 (ATCS Mustard Weed)
   

   Use

   Tags

   cogGFP

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. STARR-seq luciferase validation vector_mP_CMV

    Plasmid

    #99313

    Purpose

    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    CMV enhancer
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPHR-EYFP-NLS-VP16

    Plasmid

    #103822

    Purpose

    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-EYFP-VP16 (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VP16: G126T (silent, G42G)

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGWB515_35S-HA-(ΔTP)DM3(Col-0) D332A Q345A N349A

    Plasmid

    #229716

    Purpose

    Stable transformation or transient expression of gene of interest in plants

    Depositor

    Eunyoung Chae

    Article

    Wan et al Molecular Cell, Volume 85, Issue 14

    Insert

    DM3(Col-0) (AT3G61540 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    amino acid 1-52 were deleted, D332A Q345A N349A

    Promoter

    Available since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMDC32B-NbmiR482aTS-B/c

    Plasmid

    #227967

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    NbmiR482aTS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S and None

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pICH86966::AtU6p::sgRNA:NbCysP6

    Plasmid

    #223217

    Purpose

    Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

    Depositor

    Tsepo Tsekoa

    Article

    Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

    Insert

    NbCysP6 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6 promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNK7189

    Plasmid

    #219759

    Purpose

    Vector for expression At4CL2 in mammalian cells (HEK293NT)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    At4CL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PHR-GFP-VP16

    Plasmid

    #183927

    Purpose

    Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-VP16 (CRY2, UL48 Mustard Weed, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmiRFP2-P2A-HO1-N1

    Plasmid

    #178966

    Purpose

    Expresses miRFP2 and HO1 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2-P2A-HO1
    

    Use

    Tags

    no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSUMO His6 SUMO SnRK2.3 (19-361)

    Plasmid

    #177846

    Purpose

    Bacterial Expression of SnRK2.3

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTD73

    Plasmid

    #172897

    Purpose

    Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on chromosome I

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTD81

    Plasmid

    #172902

    Purpose

    Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

    Depositor

    David Reiner

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    TIR-1 (TIR1 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. deltaVP1 + VP2C-MIOX

    Plasmid

    #166680

    Purpose

    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. wtVP1 + VP2C-GFP

    Plasmid

    #166674

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFP
    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)

    Plasmid

    #110143

    Purpose

    Transformation and expression of Csy4 and cogDsRed proteins in plants

    Depositor

    Dominique Loque

    Article

    Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

    Insert

    Csy4 (ATCS Mustard Weed)
    

    Use

    Binary vector

    Tags

    cogDsRed

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTKan-p35S::Csy4-pC4H::cogGFP (C44, JBEI-16338)

    Plasmid

    #110144

    Purpose

    Transformation and expression of Csy4 and cogGFP proteins in plants

    Depositor

    Dominique Loque

    Article

    Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

    Insert

    Csy4 (ATCS Mustard Weed)
    

    Use

    Tags

    cogGFP

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. STARR-seq luciferase validation vector_ORI_GTSE1

    Plasmid

    #99303

    Purpose

    Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr22: 46718429 -46719913
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. STARR-seq luciferase validation vector_mP_GTSE1

    Plasmid

    #99304

    Purpose

    Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr22: 46718429 -46719913
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. STARR-seq luciferase validation vector_SCP1_GTSE1

    Plasmid

    #99305

    Purpose

    Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr22: 46718429 -46719913
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. STARR-seq luciferase validation vector_mP_IGF1R

    Plasmid

    #99307

    Purpose

    Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr15: 99439352 -99440791
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. STARR-seq luciferase validation vector_SCP1_IGF1R

    Plasmid

    #99308

    Purpose

    Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr15: 99439352 -99440791
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1mLCAD_K42Q_FLAG

    Plasmid

    #24487

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lys42Gln

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA3.1mLCAD_K42A_FLAG

    Plasmid

    #24488

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lys42Ala

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pOPINE GFP nanobody:Halo:His6

    Plasmid

    #111090

    Purpose

    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

    Depositor

    Lennart Wirthmueller

    Article

    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

    Insert

    GFP Nanobody
    

    Use

    Tags

    Halo and His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKI_ΩBBMVP16&SRDXWUSm1

    Plasmid

    #220349

    Purpose

    Expresses BBM-VP16 and SRDX-WUSm1 in plant cells

    Depositor

    Tomoko Igawa

    Article

    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

    Inserts

    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    

    Use

    Tags

    Superman Repression Domain X (SRDX) and VP16 tran…

    Expression

    Plant

    Mutation

    two amino acid substitution in WUS-box: L255A L25…

    Promoter

    CaMV 35S and RPS5A

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LAMP-mCherry-CRY2

    Plasmid

    #102249

    Purpose

    Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    LAMP-mCherry-CRY2 (LAMP1, CRY2 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-OptoSTIM1

    Plasmid

    #70159

    Purpose

    Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1 (STIM1 Human, Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-OptoTGFBRs

    Plasmid

    #118942

    Purpose

    Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

    Depositor

    Won Do Heo

    Article

    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

    Insert

    OptoTGFBRs (Tgfb1, Tgfbr2 Mouse, Mustard Weed)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. hSyn-DIO-somBiPOLES-mCerulean

    Plasmid

    #154951

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Has Service

    AAV Retrograde, AAV5, and AAV9

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CaMKII-somBiPOLES-mCerulean

    Plasmid

    #154948

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Has Service

    AAV Retrograde, AAV5, and AAV9

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAC1-V16-eGFP

    Plasmid

    #122033

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

    Plasmid

    #216244

    Purpose

    Express AtAFB2.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A-mCherry
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCMV-OptoFGFR1

    Plasmid

    #59776

    Purpose

    Expresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused protein

    Depositor

    Won Do Heo

    Article

    Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

    Insert

    OptoFGFR1 (FGFR1 Human, Mustard Weed)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAGT9569

    Plasmid

    #219431

    Purpose

    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Tags

    PapE-4LF2

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pYEE

    Plasmid

    #159750

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid

    #84243

    Purpose

    Expresses ABA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSUMO His6 SUMO SnRK2.6 (11-362)

    Plasmid

    #177847

    Purpose

    Bacterial Expression of SnRK2.6

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.6 (OST1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. lentiMPH v2 (Neo)

    Plasmid

    #92065

    Purpose

    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Insert

    MS2-P65-HSF1_2A_Neo (HSF1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pYUU

    Plasmid

    #159751

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. kanMX6-ins2

    Plasmid

    #195039

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. natMX6-ins5

    Plasmid

    #195042

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    NatR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pWZLblasti-HA-ERK2 GOF

    Plasmid

    #53174

    Purpose

    Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Human, Mustard Weed)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R67S/D321N

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only