We narrowed to 8,206 results for: Dos
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Plasmid#108484Purposeinducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFPDepositorInsertCenpB-dCEN-INCENP (INCENP Human)
TagsEGFP and VSVExpressionMammalianMutationCEN box replaced by CenpB 1-166PromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5A0-T
Plasmid#92016PurposeExpresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219VPromoterJ23102 Promoter (from Anderson promoter library) …Available SinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur
Plasmid#69052Purposelentiviral expression of IKZF2 variant 2 with HA tagDepositorInsertIKZF2 (IKZF2 Human)
UseLentiviralTagsHAExpressionMammalianMutationsplice variant 2PromoterUBCAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-hLYZ
Plasmid#83924PurposeCo-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..DepositorAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBiex1-SYNAC
Plasmid#82710PurposeSynthetic soluble adenylyl cyclase FRET sensor and cAMP generatorDepositorTagsFLAG, mCerulean, and mCitrineExpressionBacterial and InsectPromoterT7Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
USP19 sgRNA1
Plasmid#78585Purposedelete USP19 gene in human cellDepositorInsertUSP19 sgRNA1
UseCRISPRExpressionMammalianPromoterCBhAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET-GST-05230
Plasmid#67874PurposeE.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.DepositorInsertshy1
TagsGSTExpressionBacterialPromoterT7Available SinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mB
Plasmid#60985PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EBFP2.DepositorInsertEBFP2
UseZebrafishTagsmembrane tagPromoterBactin2Available SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR-BLINK2
Plasmid#117075PurposeBLINK2 gene in pDONR vector for Gateway cloning systemDepositorInsertBLINK2
UseGateway cloning systemMutationglutamine 112 changed into aspartate (original LO…Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR003-sgTP53-1
Plasmid#118019PurposeConstitutive expression of sgRNA targeting human TP53DepositorAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3
Plasmid#106205PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVMutationGltI: A184V + mRuby3 fusionPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ATF2_WT
Plasmid#82889PurposeGateway Donor vector containing ATF2, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Pansio
Plasmid#214441PurposeBxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0 Tp
Plasmid#188619PurposeMutated pTA-Mob 2.0 Backbone in TraJ Promoter RegionDepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidExpressionBacterial and YeastAvailable SinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC841 - pAAV minP secNLuc
Plasmid#82498PurposeLongitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivoDepositorInsertsecNLuc
UseAAVExpressionMammalianPromoterc-Fos minimal promoterAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC840 - pAAV 5xATF6 secNLuc
Plasmid#82497PurposeLongitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivoDepositorInsertsecNLuc
UseAAVExpressionMammalianPromoter5x(ATF6 binding site) c-Fos minimal promoterAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV106-pTDH3-mCherry-10xSumo-6xSIM
Plasmid#116928PurposeYeast integrative vector for expression of the SUMO-SIM complexDepositorInsert10xSUMO-6xSIM
TagsmCherryExpressionYeastPromoterTDH3Available SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HA-DLC1-i4
Plasmid#99534PurposeDLC1-isoform 4 expression plasmidDepositorAvailable SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6f
Plasmid#62808PurposepPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6f-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE Tight ArchT-GFP
Plasmid#79627PurposeTetracycline-dependent expression of ArchT-EGFP in mammalian cellsDepositorInsertArchT
TagsEGFPExpressionMammalianMutationArchT is a more sensitive version of Arch (archae…PromoterTet responsive Ptight promoterAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKK6 (S207E,T211E)-pcw107
Plasmid#64625Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
SmoM2 (W535L)-pcw107
Plasmid#64627Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gli2 truncation-pcw107-V5
Plasmid#64626Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
BCL-XL-pcw107-V5
Plasmid#64631Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Caspase-3 (C163A)-pcw107-V5
Plasmid#64633Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCASP3 (transcript variant beta) (CASP3 Human)
UseLentiviralTagsV5MutationC163APromoterPGKAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pHR_PGK_antiCD19_eZ3_Gal4VP64
Plasmid#169917PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralExpressionMammalianMutationAdditional EGF repeat between the extracellular d…PromoterPGKAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBAD-yhjH
Plasmid#111613PurposeArabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjHDepositorInsertyhjH
ExpressionBacterialMutationA52VAvailable SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA
Plasmid#82705PurposeAAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertU6/TO gRNA Expression Cassette Containing Tet2 gRNA
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT1
Plasmid#73943PurposeXenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tagDepositorInsertPepT1 (SLC15A1 Human)
UseMake rna for xenopus oocyte injectionTagsFlagPromoterbeta globinAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT2
Plasmid#73944PurposeXenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tagDepositorInsertPepT2 (SLC15A2 Human)
UseMake rna for xenopus oocyte injectionTagsFlagPromoterbeta globinAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
HcRed-pcw107-V5
Plasmid#64647Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsV5PromoterPGKAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-saCBE N-terminal
Plasmid#137182PurposeAAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNADepositorInsertv5 AAV-saCBE N-terminal
UseAAVMutationCas9 D10APromoterCbhAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S
Plasmid#106177PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184S
UseAAVMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterhSynapsinAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc IRES neo
Plasmid#98294PurposeFor constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance geneDepositorInsertsFirefly luciferase
IRES
Neomycin resistance gene (NeoR)
UseLentiviral and LuciferaseAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MAPK7_WT
Plasmid#82925PurposeGateway Donor vector containing MAPK7, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFB2G
Plasmid#195290PurposeBicistronic HSV-TK and mTagBFP resistance for positive/negative selectionDepositorInsertHSV-TK 2A mTagBFP
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA
Plasmid#168305Purposemediated knock-in of sgRNA precursorDepositorInsertSaKKH-EGFP-U6-sgRNA
UseAAVExpressionMammalianPromoterEF1alpha, CBhAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-IBV-3CL
Plasmid#160288PurposeAllows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-Bat-CoV-HKU9-3CL
Plasmid#160284PurposeAllows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-ABEmax C-terminal
Plasmid#137174PurposeExpresses the C-terminal split-intein fragment of ABEmax from the CMV promoterDepositorInsertNpu-ABEmax_C-terminal
ExpressionMammalianPromoterCMVAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-ABEmax N-terminal
Plasmid#137173PurposeExpresses the N-terminal split-intein fragment of ABEmax from the CMV promoterDepositorInsertNpu-ABEmax_N-terminal
ExpressionMammalianMutationCas9 D10APromoterCMVAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX303-PCDH7-a
Plasmid#108538PurposeExpresses human PCDH7 transcript variant a in mammalian cellsDepositorAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-3XFLAG
Plasmid#105784PurposeExpression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-3XFLAGExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Isl1
Plasmid#72696PurposeDoxycyline regulated expression of Isl1 in mammalian cellsDepositorAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-myo1e
Plasmid#248918PurposeExpresses EGFP-tagged human myosin 1e in mammalian cellsDepositorAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHREP-LP
Plasmid#240432PurposeEmpty landing pad vector with two Bxb1 recombination sites for RMCE and LNGFR + EGFP-HygroR for sorting/selection.DepositorInsertsLNGFR
EGFP
TagsHygromycin ResistanceExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHREP-KD-2
Plasmid#240441PurposeSleeping Beauty vector for inducible expression of an shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
Full Length Rat Bassoon
Plasmid#250160PurposeExpresses Rat Bassoon in mammalian cellsDepositorAvailable SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
pVP077
Plasmid#222029PurposeEmpty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…MutationAll Esp3i sites domesticatedAvailable SinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits