We narrowed to 6,273 results for: nls
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pKMD106e - intein-Cas9(S219)
Plasmid#64190PurposeExpresses intein-Cas9(S219) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianPromoterCMVAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
DCIP
Plasmid#193860PurposeEncodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.DepositorInsertNLS-mCherry-GFP1-10
TagsSV40 NLSExpressionPlantPromoter35SAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
E407V Nuclear Myosin I YFP
Plasmid#60242PurposeExpresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activitiesDepositorAvailable SinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1(TYCV)(BB) (pY211)
Plasmid#89352PurposeExpresses humanized AsCpf1 TYCV PAM variant and crRNA guideDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCBhAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH-dCas9-PBE
Plasmid#98162PurposeExpresses CRISPR base editor in riceDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseRiceExpressionPlantPromoterUbiAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuc-Nb80
Plasmid#205519PurposeNuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.DepositorInsertnuc-Nb80
TagsNuclear localization signal (NLS) and mCherryExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1(TATV)(BB) (pY221)
Plasmid#89354PurposeExpresses humanized AsCpf1 TATV PAM variant and crRNA guideDepositorInsertCpf1 RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCBhAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB22
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas5
Plasmid#178878PurposeExpression of human codon-optimized Nlacas5 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas5c-bGH polyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas8
Plasmid#178880PurposeExpression of human codon-optimized NlaCas8 with N terminal NLS and HA tagDepositorInsertEF1a-NlaCas8c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas11
Plasmid#178882PurposeExpression of human codon-optimized NlaCas11 with N terminal NLS and HA tagDepositorInsertEF1a-NlaCas11c-bGH polyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas7
Plasmid#178879PurposeExpression of human codon-optimized Nlacas7 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas7c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-dCas9-PBE
Plasmid#98159PurposeExpresses CRISPR base editor in maizeDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseMaizeExpressionPlantPromoterUbiAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES_mScarleti_Sec23A
Plasmid#117273PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23ADepositorInsertsTagsmScarlet-iExpressionMammalianMutationHuman Sec23A; Sequencing showed a mutation in Sec…PromoterCMV and IRESAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
p4459 FLAG-hPLIC-1 NTF
Plasmid#8664DepositorInserthPLIC-1 (UBQLN1 Human)
TagsFLAGExpressionMammalianMutationN-terminal fragment: amino acids 1-112 of hPLIC-1…Available SinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
SpRY
Plasmid#198544PurposeExpresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSDDepositorInsertSpRY
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
OmEF1aCas9P2ApuroSB
Plasmid#165484PurposeStable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cellsDepositorInsertZebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
UseCRISPR; TransposonPromoterOreochromis mossambicus EF1 alpha promoter (OmEF1…Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
A3Bmax
Plasmid#207167PurposeExpress full-length human A3B in a BE4max-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
TagsNLS and NLS, 6x HisExpressionMammalianPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialPromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-PBE
Plasmid#98161PurposeExpresses CRISPR base editor in protoplastsDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
ExpressionPlantPromoterUbiAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-ΔUGI
Plasmid#119770PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)DepositorInsertAPOBEC3A-nCas9-ΔUGI-NLS
TagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM2-rPMCA2a
Plasmid#47762Purposemammalian expression of rat PMCA2aDepositorAvailable SinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET42b-A3A-PBE-ΔUGI
Plasmid#119771PurposeTo express the recombinant APOBEC3A in vitroDepositorInsertAPOBEC3A-nCas9-NLS
Tags6*HIS and NLSExpressionBacterialMutationnCas9 has a mutation D10A in wild type SpCas9PromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PlmdCas12e KRAB
Plasmid#188506PurposeExpresses FLAG tagged PlmdCas12e KRABDepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-KRAB-NLSExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP2266
Plasmid#70705PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialPromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16
Plasmid#89872Purposeexpresses CB1R-iTango component in mammalian cellsDepositorInsertCB1R-V2tail-TevN-BLITz1-TetR-VP16
ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-NE-EGFP-IRES-Pac
Plasmid#183617PurposeMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.DepositorInsertEGFP
TagsHuman EMD transmembrane domain and NLSExpressionMammalianPromoterCAGAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_K5S
Plasmid#98679PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5SDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
Tags6xHisExpressionBacterialMutationK1838S, K1859S, K1866S, K1873S, K1887SAvailable SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD38-52
Plasmid#98685PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
Tags6xHisExpressionBacterialMutationheptads 38-52, residues 1853-1970Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF173
Plasmid#96999PurposeNLS-GFP-lacZ leu1 insertion vectorDepositorInsertlacZ
TagsNLS-GFPExpressionYeastPromoterPnmt1Available SinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
kank2_pJet1.2
Plasmid#121980Purposefor generating kank2 riboprobes for WISH in zebrafishDepositorAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE(K294A)
Plasmid#82461PurposeExpression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cellsDepositorInsertmCE (K294A) (Rngtt Mouse)
TagsmycExpressionMammalianMutationLysine 294 changed to AlaninePromoterCMVAvailable SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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RNAPII_CTD27-37
Plasmid#98686PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
Tags6xHisExpressionBacterialMutationheptads 27-37, residues 1773-1852Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc NES- mCE(K294A)
Plasmid#82464PurposeExpresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)DepositorInsertNES mRNA capping enzyme (Rngtt HIV, Mouse)
TagsmycExpressionMammalianMutationK294APromoterCMVAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES mYFP
Plasmid#130713PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-G97-NLS IRES mYFP
TagsNLS and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-F98 IRES mYFP
Plasmid#130712PurposePlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-F98-NLS IRES mYFP
TagsNLS and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
kank4_pJet1.2
Plasmid#121982Purposefor generating kank4 riboprobes for WISH in zebrafishDepositorAvailable SinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD43-52
Plasmid#98684PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
Tags6xHisExpressionBacterialMutationhepatds 43-52, residues 1888-1970Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCGT003_nuclear-mKate2
Plasmid#248085PurposeConstitutive expression of NLS-tagged mKate2 from EF1-alpha promoter (for lentiviral packaging)DepositorInsertmKate2
UseLentiviralTagsSV40 NLSExpressionMammalianPromoterEF1-alphaAvailable SinceNov. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCGT002_nuclear-EGFP
Plasmid#248084PurposeConstitutive expression of NLS-tagged EGFP from EF1-alpha promoter (for lentiviral packaging)DepositorInsertEGFP
UseLentiviralTagsSV40 NLSExpressionMammalianPromoterEF1-alphaAvailable SinceNov. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCGT001_nuclear-mOrange2
Plasmid#248083PurposeConstitutive expression of NLS-tagged mOrange2 from EF1-alpha promoter (for lentiviral packaging)DepositorInsertmOrange2
UseLentiviralTagsSV40 NLSExpressionMammalianPromoterEF1-alphaAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFC482
Plasmid#246439PurposeThe plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signalDepositorInsertCasE
TagsNLSExpressionMammalianMutationSapI domesticatedPromoterEF-1aAvailable SinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLP110_AAV Scramble Control
Plasmid#239419PurposeNegative control AAV vector for SaCas9-based CRISPR KODepositorInsertScramble control
UseAAVAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS1369
Plasmid#239828PurposeWT OrufIscB mammalian expressionDepositorInsertOrufIscB
TagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceJuly 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN103
Plasmid#233860PurposeMoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1DepositorInsertpTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN104
Plasmid#233861PurposeMoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1DepositorInsertpTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAL007
Plasmid#233866PurposeMoClo-YTK URA multigene plasmid with MCS; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2DepositorInsertpTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only
