-
Plasmid#174396PurposepBM23 contained pyrF and mini-CRISPR.DepositorInsertpyrF-CS
UseTemplate for cloning fragments for chromosomal in…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup35-EcoRI
Plasmid#87332PurposeTo express shRNA against human Nup35DepositorInsertshRNA against human Nup35
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEntra1a-mTurquoise2-3xrGBD
Plasmid#176137PurposeVisualization of active RhoDepositorInsertrhotekin (Rtkn Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_H2B
Plasmid#65702PurposeExpresses YNL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
roGFP_iL_pQE30
Plasmid#48633PurposeBacterial expression vector encoding for the 6XHis tagged redox probe roGFPiL (roGFP C147, L147b, C204)DepositorInsertroGFP1
UseTagsHisTagExpressionMutationinsertion L147BPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-MsCHI
Plasmid#73392PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE93 mFZ3-CS2+
Plasmid#16804DepositorInsertmFZ3-CS2+ (Fzd3 Mouse)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS-BtH
Plasmid#133392PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCx55.5-TrpR
Plasmid#86373Purposefor zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherryDepositorInsertTrpR
UseZebrafishTagsExpressionMutationPromotercx55.5Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Vv4CL-CmCHS-MsCHI
Plasmid#73402PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsVv4CL
CmCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.242
Plasmid#73754PurposeNabi2.242 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.242
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
CBM9-(PT4)P
Plasmid#171708PurposeCarries CBM9 fusion tagDepositorInsertCellulose Bindind Protein 9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pILGFPQ3
Plasmid#83558PurposeUsed to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.DepositorInsertGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA125
Plasmid#74249PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
UseTagsExpressionBacterial and YeastMutationPromoterpSTL1Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-10
Plasmid#160710PurposeFor C-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-8
Plasmid#160708PurposeFor N-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-7
Plasmid#160707PurposeFor N-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM53
Plasmid#154156PurposeFirefly luciferase under the control of 8 copies of the Aedes aegypti Rel1A NF-κB siteDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoter8 copies of the Aedes Aegypti Rel1A NF-κB promoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Chronos(M140T)-mRuby2-ST
Plasmid#109129PurposeModified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChronos(M140T)-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_LAMP1
Plasmid#65686PurposeExpresses LAMP1-ONL in mammalian cellsDepositorInsertLAMP1
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTRdeltaGFPmirGE-DroshaT1.5.5
Plasmid#61084PurposepENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.DepositorInsertdeltaGFP
UseRNAiTagsExpressionMutationPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_H2B
Plasmid#65704PurposeExpresses ONL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMX Vav1 Y3F/DH* (L213Q) uGFP
Plasmid#14559DepositorInsertVav1 (VAV1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationY3F (Y142, Y160, Y174)/DH* (L213Q)PromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
XE107 XPKC alpha-GFP-CS2+
Plasmid#16743DepositorInsertPKC-alpha (prkca Frog)
UseXenopus expressionTagsGFPExpressionMutationPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMX Vav1 Y3F/NSH3* (P651L) uGFP
Plasmid#14562DepositorInsertVav1 (VAV1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationY3F/NSH3* (P651L)PromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
XE96 mFZ7-CS2+
Plasmid#16801DepositorInsertFZ7 (Fzd7 Mouse)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_wt_Venus_173
Plasmid#39815DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE intramol-1m
Plasmid#13551DepositorInsertFLIP-E intramol A207W (gltI E. coli)
UseTagsEYFPExpressionBacterialMutationA207W, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_105_Venus_229
Plasmid#39822DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_PTS2
Plasmid#65677PurposeExpresses PTS2-ONL in mammalian cellsDepositorInsertperoxisomal targeting signal 2
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMX Vav1 Y3F/SH2* (R696K) uGFP
Plasmid#14565DepositorInsertVav1 (VAV1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationY3F/SH2* (R696K)PromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
mOC-STAMP_pcDNA3.1 D/V5-His-TOPO
Plasmid#73448PurposeExpress mouse OC-STAMP in mammalian cellsDepositorInsertOcstamp (Ocstamp Mouse)
UseTagsV5-6xHisExpressionMammalianMutationN162DPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
RV-Syn-Dio-mTdt
Plasmid#87667Purposeretroviral vector encoding cre dependent expression of membrane TdtomatoDepositorInsertMem-Tdtomato
UseRetroviralTagspalmitylationExpressionMutationPromoterSynapsinAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMX Vav1 Y3F/CSH3* (P833L) uGFP
Plasmid#14566DepositorInsertVav1 (VAV1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationY3F/CSH3* (P833L)PromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCNL-C1_H2B
Plasmid#65703PurposeExpresses CNL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB690
Plasmid#60134PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup93-SpeI
Plasmid#87331PurposeExpression of shRNA targeting at Nup93DepositorInsertshRNA against human Nup93
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-R1ACR_653-EYFP
Plasmid#111079PurposeAnion channelrhodopsin from Rhodomonas sp. for expression in mammalian cellsDepositorInsertAnion channelrhodopsin R1ACR_653
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Pc4CL-PhCHS-CmCHI
Plasmid#73397PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsPc4CL
PhCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT3
Plasmid#98304PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoterDepositorInsertTRPL3AcNES1>TRPL41B
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLN553 (FuGW-S(MAFK)p-Sponge7)
Plasmid#105203PurposeModule 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)DepositorInsertS(MAFK)p-Sponge7
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup88-HindIII
Plasmid#87329PurposeTo express shRNA against human Nup88DepositorInsertshRNA against human Nup88
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_105_Venus_wt
Plasmid#39818DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
UseTags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMX Vav1 Y3F/Grb2bindb* (P657A) uGFP
Plasmid#14563DepositorInsertVav1 (VAV1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationY3F/Grb2bindb* (P657A)PromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_227_Venus_173
Plasmid#40016DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-mTmod3-1-325_domain
Plasmid#70756Purposemouse Tropomodulin3, 1-325 domain for bacterial expressionDepositorInsertTropomodulin3 1-325 AA fragment (Tmod3 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_227_Venus_229
Plasmid#39837DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSR21
Plasmid#69156Purposeread-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr XDepositorInsertsmCherry
TagBFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterrps-27Available sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB754
Plasmid#60157PurposeGreen beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterLYS9prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only