We narrowed to 18,352 results for: Tec

1. pRS423-CUP1-His-Ubi

   Plasmid

   #99541

   Purpose

   Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   UBI3(1-76) (RPL40A Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YEp195-CUP1-His-Ubi

   Plasmid

   #99540

   Purpose

   Yeast episomal vector for expression of His-tagged ubiquitin; URA3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   UBI3(1-76) (RPL40A Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. YEp181-CUP1-His-Ubi

   Plasmid

   #99539

   Purpose

   Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   UBI3(1-76) (RPL40A Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUCBB-oriT

   Plasmid

   #32556

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   oriT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAGD289

   Plasmid

   #228113

   Purpose

   pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Kva1 retron, ncRNA with 90 bp rpoB donor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 BoMo N-1F

   Plasmid

   #203761

   Purpose

   For expression of N-flag tagged BoMo R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMR-A-6

   Plasmid

   #138520

   Purpose

   P3d

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   T7-RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

   Plasmid

   #55217

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NM9-SpCas9-NLS3

   Plasmid

   #128177

   Purpose

   Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   SpCas9-NLS3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pPGK1

   Available since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFPL-Rh

    Plasmid

    #61649

    Purpose

    Gateway vector for generating red fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    monomeric red fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHCKan-yibDp-GFPuv

    Plasmid

    #127078

    Purpose

    Low phosphate inducible yibD gene promoter expressing GFPuv reporter

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSET FLIPE-600n

    Plasmid

    #13537

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E WT
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMRBAD-Z-CGFP

    Plasmid

    #52735

    Purpose

    L-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    Z+CGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPomyces-SaCas9

    Plasmid

    #129553

    Purpose

    Contains codon-optimized SaCas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    SaCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB5791

    Plasmid

    #106126

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTW199b2

    Plasmid

    #136928

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAYP (pDre)

    Plasmid

    #62709

    Purpose

    Mammalian expression of Dre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    Dre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET11a-Z-NGFP

    Plasmid

    #52734

    Purpose

    IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    NGFP+Z-fusion
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Marathon-RT for Split-PE (pEH59)

    Plasmid

    #190108

    Purpose

    Expresses Marathon-RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTKhc-yibDp-GFP-cp6

    Plasmid

    #134938

    Purpose

    Low phosphate inducible circular permutation of GFP

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    GFPcp6
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-CD40L-mgSrtA

    Plasmid

    #125795

    Purpose

    for co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    CD40L-mgSrtA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAG1244 p65-mCherry-FIREtag

    Plasmid

    #205175

    Purpose

    Expresses p65-mCherry-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    p65-mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCfB6576

    Plasmid

    #106134

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PlayBack-Ef1a-Bamh1

    Plasmid

    #203308

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfB4787

    Plasmid

    #106147

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. YSBE 610 : pHYG_6xHA

    Plasmid

    #92091

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    6xHA
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDYU1G/γ-tubulin-mEGFP

    Plasmid

    #154301

    Purpose

    Expresses γ-tubulin–mEGFP from actin15 promoter in Dictyostelium discoideum

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Insert

    γ-tubulin
    

    Use

    Dictyostelium expression

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    actin15

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ABE-RA3.0

    Plasmid

    #208301

    Purpose

    Expresses ABE-RA3.0 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA3.0-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAFi-dHdrA

    Plasmid

    #184907

    Purpose

    carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cm-sgRNA-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAG1238 PINK1-mCherry-FIREtag

    Plasmid

    #205174

    Purpose

    Expresses PINK1-mCherry-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    PINK1-mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRBBm34

    Plasmid

    #48114

    Purpose

    Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megaterium

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

    Insert

    gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Ca-FLARE (protease)

    Plasmid

    #92214

    Purpose

    protease component of FLARE

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    CaM-V5-TEVp(Δ220-242)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EGFP-Septin 11 isoform II

    Plasmid

    #120317

    Purpose

    Septin 11 isoform II mammalian expression (EGFP-tag at the N-terminus)

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pENTR-L1-PGK-HygTK-L2

    Plasmid

    #141006

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    HygTK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

    Plasmid

    #48295

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCHARGE3

    Plasmid

    #24658

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    Turbo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTlpA_mCherry

    Plasmid

    #229146

    Purpose

    Promoter strength evaluation of PtlpA

    Depositor

    Shrikrishnan Sankaran

    Article

    Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No

    Promoter

    PtlpA

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBW842_pCAG-pExpr-FP-DECODER

    Plasmid

    #87551

    Purpose

    Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mtagBFP, EGFP, iRFP720, mRuby2
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDRFLIP38

    Plasmid

    #65516

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edCit-attR1 and attR2-edCer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

    Plasmid

    #55219

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCTCON2-BXG

    Plasmid

    #158127

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    mTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCfB2189

    Plasmid

    #67532

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBT138b-proD

    Plasmid

    #138516

    Purpose

    P3e

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. SaABEmax

    Plasmid

    #119814

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pBAD-HisD-CreiLOV

    Plasmid

    #192817

    Purpose

    Expresses CreiLOV in bacteria

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pFW11-ffluc

    Plasmid

    #128487

    Purpose

    Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    firefly luciferase gene
    

    Use

    Allelic replacement vector

    Tags

    spy0535 downstream flanking region and spy0535 up…

    Expression

    Mutation

    Promoter

    synthetic

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLb-Cpf1-pGL3-U6-sgRNA

    Plasmid

    #107682

    Purpose

    Expresses LbCpf1 crRNA in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. PlayBack-CMV-Kpn1

    Plasmid

    #203302

    Purpose

    For expression of eukaryotic genes of interest with CMV promoter with Kpn1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pTR47m4

    Plasmid

    #104068

    Purpose

    Luciferase Reporter for Formaldehyde Sensing

    Depositor

    David Liu

    Article

    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

    Inserts

    xluxAB
    frmR
    frm Promoter m4
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    23r and 25th nucleotides in sequence mutated from…

    Promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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