We narrowed to 32,761 results for: IND

1. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHCKan-yibDp2-GFPuv

   Plasmid

   #139083

   Purpose

   Low phosphate inducible promoter yibDp2 for GFP expression

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

   Insert

   yibDp2-GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 7h sgRNA for 4-μHOM reporter

   Plasmid

   #113625

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7h sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pET28a-TRBP

   Plasmid

   #50351

   Purpose

   bacterial expression of TRBP

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

   Insert

   TRBP (TARBP2 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA5-puro-eGFP-HEC1

   Plasmid

   #114022

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB-Mycn

   Plasmid

   #60667

   Purpose

   PiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoter

   Depositor

   James Thomson

   Article

   Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

   Insert

   Mycn (Mycn Mouse)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-cT3G-cERP2-TERF1

   Plasmid

   #222542

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of TERF1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   TERF1 (TERF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 7i sgRNA for 4-μHOM reporter

   Plasmid

   #113626

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7i sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

9. PC1-29-9-2_GAL4-VHL

   Plasmid

   #200434

   Purpose

   Expresses the GAL4 DNA Binding domain linked to the VHL E3 Ubiquitin Ligase

   Depositor

   Xue Gao

   Article

   Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

   Insert

   GAL4-VHL
   

   Use

   Tags

   GAL4, VHL

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHUC-JEDI-1P

    Plasmid

    #202619

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P under the HuC promoter for pan neuronal expression in zebrafish; JEDI-1P sequence codon-optimized for zebrafish expression

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    HuC

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmScarlet-H_NES_C1

    Plasmid

    #85061

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGal4AD-CIBN

    Plasmid

    #28246

    Purpose

    Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIB1 (1-171aa)
    

    Use

    Tags

    Gal4AD

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Yellow Cameleon-Nano15/pcDNA3

    Plasmid

    #51961

    Purpose

    mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

14. rASAP

    Plasmid

    #178205

    Purpose

    Ratiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.

    Depositor

    Stefan Heinemann

    Article

    Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

    Insert

    rASAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 3xnls-G-Ca-FLITS

    Plasmid

    #191463

    Purpose

    Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p416 25Q GPD

    Plasmid

    #1177

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TB:4xHRE_RE:MiniP

    Plasmid

    #178313

    Purpose

    4 copies of the HRE DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    4 copies of the heat shock DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMA3288

    Plasmid

    #46885

    Purpose

    Lentiviral vector encoding Tet-inducible mitochondrially targeted Wild Type human Uracil-N-glycosylase

    Depositor

    Mikhail Alexeyev

    Article

    Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

    Insert

    Uracil-N-glycosylase WT (UNG Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-Tight

    Available since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p416 103Q GPD

    Plasmid

    #1180

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pTRIPZ TREX1-KI

    Plasmid

    #127701

    Purpose

    For knocking in TREX1 in mouse - Doxycyclin inducible

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    mouse TREX1 cDNA (Trex1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBAD-GINKO1

    Plasmid

    #113111

    Purpose

    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Use

    Tags

    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. c3GIC9

    Plasmid

    #62191

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-CAG-iGECInano

    Plasmid

    #186188

    Purpose

    AAV vector packaging small FRET-based near-infrared genetically-encoded calcium indicator

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

    Insert

    iGECInano
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Prab-3::NLS::GCaMP6s

    Plasmid

    #68119

    Purpose

    Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    rab-3::NLS::GCaMP6s
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p416 72Q GPD

    Plasmid

    #1179

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 72Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

26. pmScarlet-I_peroxisome_C1

    Plasmid

    #85065

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Peroxisomal targeting sequence
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBAD-T-GECO1

    Plasmid

    #191616

    Purpose

    Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

    Depositor

    Robert Campbell

    Article

    Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

    Insert

    T-GECO1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Rc/CMV cyclin C

    Plasmid

    #8961

    Depositor

    Bob Weinberg

    Article

    Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

    Insert

    cyclin C (CCNC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

29. pAAV-Syn-VARNAM

    Plasmid

    #115554

    Purpose

    Red fluorescent voltage indicator for neuronal expression

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

    Insert

    VARNAM
    

    Use

    AAV

    Tags

    Golgi and ER export signal

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET151-hUCH37 (ISF1)

    Plasmid

    #61929

    Purpose

    Expression of hUCH37 (ISF1) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pJM251 (miniCTX1-araC-ParaBAD)

    Plasmid

    #110567

    Purpose

    Integration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    araC-ParaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araC-ParaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMGS58

    Plasmid

    #135311

    Purpose

    A HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminal

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    HygR-P2A-AID
    

    Use

    N terminal tagging plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CMV-CAR-GECO1

    Plasmid

    #45493

    Purpose

    Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    CAR-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAID 1.1 N-T2A-Bsr

    Plasmid

    #105985

    Purpose

    To make an auxin inducible degron mutant

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

    Insert

    T2A-Bsr
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NLS-tdPCP-GFP

    Plasmid

    #183934

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p416 103Q GAL

    Plasmid

    #1186

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p2H12

    Plasmid

    #221142

    Purpose

    cdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BRAC/pcDNA3

    Plasmid

    #51967

    Purpose

    mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

    Insert

    BRAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pYX233

    Plasmid

    #163762

    Purpose

    Empty vector for Gal-inducible yeast expression

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Type

    Empty backbone

    Use

    Tags

    Hemagglutinin (HA) tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p153 pPr-cre

    Plasmid

    #8403

    Purpose

    Expression of Cre from tissue-specific, androgen-inducible probasin promoter.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    probasin driven Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    probasin promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

41. p416 25Q GAL

    Plasmid

    #1185

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCFD3-frame_selector_0

    Plasmid

    #127553

    Purpose

    Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 0
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pFSW Syt1 WT IRES GFP

    Plasmid

    #133816

    Purpose

    Encodes full-length wild type synaptotagmin 1 for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. p416 46Q GPD

    Plasmid

    #1178

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 46Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

46. pCFD3-frame_selector_1

    Plasmid

    #127554

    Purpose

    Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGinB-8X(GGS)-dCas9-FLAG-NLS

    Plasmid

    #81205

    Purpose

    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    pGinB-8X(GGS)-dCas9-FLAG-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCFD3-frame_selector_2

    Plasmid

    #127555

    Purpose

    Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Gi-c001

    Plasmid

    #173078

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pTorPE-G-GECO1

    Plasmid

    #32466

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.0
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

    Expression

    Bacterial

    Mutation

    GCaMP3 K119I/L173Q/S404G/E430V

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge