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We narrowed to 22,079 results for: his

Showing: 3051 - 3100 of 22079 results

  2. AAP-COMP-blac-flag-his

    Plasmid
    #111001
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    aminopeptidase P (APP) (PF14_0517 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111001-plasmid-map-sequence-id-224546

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  5. pHybr_r2a>m2a(silent)-srt-his

    Plasmid
    #124807
    Purpose
    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a (Fc silent)
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    L234A/L235A/N297A
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124807-plasmid-map-sequence-id-251832

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  8. HOXA5 (human) HIS-tag pET

    Plasmid
    #8562
    Depositor
    Corey Largman
    Insert
    HOXA5 (HOXA5 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    SacI site at amino acids 1-2 was used to clone in…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  9. HOXC9 (murine) HIS-tag pET

    Plasmid
    #8559
    Depositor
    Corey Largman
    Insert
    HOXC9 (Hoxc9 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    makes protein by Western
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  12. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid
    #44072
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44072-plasmid-map-sequence-id-61692

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  23. HOXA2 (murine) HIS-tag pET

    Plasmid
    #8557
    Depositor
    Corey Largman
    Insert
    HOXA2 (Hoxa2 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    contains 5 additional amino acids from 5' un…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  24. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169180
    Purpose
    Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-His6-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    Use
    Tags
    His6 (internal tag linking nsp7 and nsp8): nsp7-H…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169180-plasmid-map-sequence-id-333998

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  25. pET28a-ybbr-HIS-ddFLN4-K10

    Plasmid
    #101718
    Purpose
    K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    ddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)
    Use
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101718-plasmid-map-sequence-id-196776

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  28. pNUT N6His K206E/K534A hTFNG

    Plasmid
    #70133
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe
    Depositor
    Anne Mason, Ross MacGillivray
    Article
    Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.
    Insert
    N6His K206e/K534A hTFNG (TF Human)
    Use
    Tags
    N-terminal signal peptide, 4 aa link, 6 His, Fact…
    Expression
    Mammalian
    Mutation
    changed Lys206 to a glutamic acid and Lys534 to a…
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70133-plasmid-map-sequence-id-129418

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  39. P47-COMP-blac-flag-his

    Plasmid
    #111000
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    secreted ookinete protein, putative (PSOP12) (PF3D7_0513700 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111000-plasmid-map-sequence-id-224544

    Enlarge

  40. SOAP-COMP-blac-flag-his

    Plasmid
    #110997
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    secreted ookinete adhesive protein (SOAP) (PF3D7_1404300 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110997-plasmid-map-sequence-id-224488

    Enlarge

  41. CTRP-COMP-blac-flag-his

    Plasmid
    #110994
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    circumsporozoite- and TRAP-related protein (CTRP) (CTRP Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110994-plasmid-map-sequence-id-224485

    Enlarge

  42. P25-COMP-blac-flag-his

    Plasmid
    #110991
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    ookinete surface protein P25 (PF10_0303 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110991-plasmid-map-sequence-id-224481

    Enlarge

  43. WARP-COMP-blac-flag-his

    Plasmid
    #110989
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    von Willebrand factor A-domain related protein (WARP) (PF08_0136b Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110989-plasmid-map-sequence-id-224479

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  49. HOXA1 (murine) HIS-tag pET

    Plasmid
    #8556
    Depositor
    Corey Largman
    Insert
    HOXA1 (Hoxa1 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    clone contains 130 AA including the homeodomain, …
    Promoter
    Available since
    July 5, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 3051 - 3100 of 22079 results