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Showing: 1861 - 1890 of 4045 results

  2. pCW57-MCS1-2A-MCS2

    Plasmid
    #71782
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71782-plasmid-map-sequence-id-222919

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  5. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  6. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid
    #26976
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV5, AAV8, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26976-plasmid-map-sequence-id-313556

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  8. CMV-LUC2CP/intron/ARE

    Plasmid
    #62858
    Purpose
    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    insertion of chimeric intron at nueotide poclsiti…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62858-plasmid-map-sequence-id-243538

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  11. lentiGuide-Hygro-mTagBFP2

    Plasmid
    #99374
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99374-plasmid-map-sequence-id-430620

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  13. pSBbi-Hyg

    Plasmid
    #60524
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60524-plasmid-map-sequence-id-456411

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  16. G-CaMP3

    Plasmid
    #22692
    Purpose
    a single-wavelength GCaMP2-based genetically encoded calcium indicator
    Depositor
    Loren Looger
    Article
    Tian et al Nat Methods. 2009 Dec;6(12):875-81.
    Insert
    G-CaMP3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N363D mutation (increased fluorescence)
    Promoter
    Available since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    22692-plasmid-map-sequence-id-241043

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  22. MSCV-Cre

    Plasmid
    #24064
    Purpose
    Retroviral coexpression of Cre and EGFP
    Depositor
    Dan Littman
    Insert
    Cre
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    MESV 5' LTR
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24064-plasmid-map-sequence-id-322907

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  23. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid
    #84561
    Purpose
    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops
    Depositor
    Robert Singer
    Article
    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d
    Insert
    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    Use
    Lentiviral
    Tags
    Flag, Suntag, oxBFP, AID (auxin-inducible degron)
    Expression
    Mutation
    Promoter
    UbC
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    84561-plasmid-map-sequence-id-331246

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  24. pSin Helper

    Plasmid
    #127694
    Purpose
    Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.
    Depositor
    Justin English, Bryan Roth
    Article
    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306
    Insert
    Sindbis Glycoproteins
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. EBFP2-N1

    Plasmid
    #54595
    Purpose
    Localization: N1 Cloning Vector, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54595-plasmid-map-sequence-id-364695

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Showing: 1861 - 1890 of 4045 results