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pCMV/SB11

Plasmid

#26552

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Use

Tags

Expression

Bacterial

Mutation

Methionine 243 to Glutamine Threonine 136 to Arg…

Promoter

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-C1-AKT-PH

Plasmid

#21218

Depositor

Tobias Meyer

Article

Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

Insert

AKT-PH

Use

Tags

GFP

Expression

Mammalian

Mutation

PH domain of AKT. (Lab plasmid WC 0002).

Promoter

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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hCas9_D10A

Plasmid

#41816

Purpose

Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9_D10A

Use

CRISPR

Tags

Expression

Mammalian

Mutation

human codon-optimized, D10A nickase

Promoter

CMV

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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NFAT/AP-1 3x luciferase

Plasmid

#11783

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

NFAT/AP-1 3x (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pSLQ1651-sgTelomere(F+E)

Plasmid

#51024

Purpose

Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

Depositor

Bo Huang, Stanley Qi

Article

Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

Insert

Optimized sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-sh1368 knockdown c-myc

Plasmid

#29435

Depositor

Bob Eisenman

Article

Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

Insert

c-Myc shRNA (MYC Human)

Use

Lentiviral and RNAi

Tags

GFP and WPRE

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1

Plasmid

#34706

Depositor

David Chan

Insert

Drp1 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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FHRE-Luc

Plasmid

#1789

Purpose

Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FHRE (FOXO3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

3 copies of FRE

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-CUL2

Plasmid

#19892

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 2 (CUL2 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pET15bpATnp

Plasmid

#121137

Purpose

This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

Depositor

Keji Zhao

Article

Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

Insert

Protein A-Tnp

Use

Tags

his

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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mCitrine-N1

Plasmid

#54594

Purpose

Localization: N1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Oliver Griesbeck, Robert Campbell, Michael Davidson, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET30a-AlkB

Plasmid

#79050

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Use

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TCF1

Plasmid

#40620

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

TCF7 (TCF7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Mito

Plasmid

#36208

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

MTS (COX8A Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lwa_Cas13a_WT

Plasmid

#91865

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LwaCas13a

Use

CRISPR

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-ABE(7.10)

Plasmid

#108382

Purpose

Mammalian expression vector for xCas9(3.7)-ABE

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-ABE(7.10)

Use

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV/SB10

Plasmid

#24551

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-EGFP-Rac1(Q61L)

Plasmid

#13720

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(Q61L) (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-TOMM20-N-10

Plasmid

#54282

Purpose

Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

TOMM20 (TOMM20 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Article

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-HA-OTUB1

Plasmid

#22551

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUB1 (OTUB1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
hIRE1a wt

Plasmid

#20744

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-3xFLAG-hphMX4

Plasmid

#20755

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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EMTB-3XGFP

Plasmid

#26741

Depositor

William Bement

Article

Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

Insert

ensconsin (MAP7 Human)

Use

Probe for microtubules

Tags

3x EGFP and Myc

Expression

Mammalian

Mutation

Only contains the microtubule binding-domain of e…

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
shp53 pLKO.1 puro

Plasmid

#19119

Purpose

3rd gen lentiviral vector for knocking down p53 gene expression

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p53 (TP53 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCXLE-hOCT3/4

Plasmid

#27076

Purpose

Non-integrating (episomal) expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

OCT3/4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK292 (mAID-mCherry2-NeoR)

Plasmid

#72830

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Neo

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK-Neo

Plasmid

#25735

Purpose

3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW

Plasmid

#14882

Depositor

David Baltimore

Article

Lois et al Science. 2002 Feb 1. 295(5556):868-72.

Insert

flap-Ub promoter-WRE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-Spike

Plasmid

#145031

Purpose

Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

SARS-CoV Spike

Use

Tags

C9 and CD5 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pQE-WT-RGVG-M6

Plasmid

#70172

Purpose

Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

Depositor

Andrew Ellington

Article

Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

Insert

RGVG-M6 T7 RNA polymerase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T5/Lac

Available since

Dec. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
OKSIM

Plasmid

#24603

Depositor

Jose Cibelli

Article

Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

Insert

Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Oct4-2A-KLF4-2A-Sox2-IRES-Myc

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV p16 INK4A

Plasmid

#10916

Depositor

Bob Weinberg

Article

Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

Insert

p16 INK4 (CDKN2A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS6-SMASh-YFP

Plasmid

#68852

Purpose

Expresses SMASh tag fused to YFP at N terminus in mammalian cells

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

SMASh-YFP

Use

Tags

SMASh tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Smad7-HA

Plasmid

#11733

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGP-CMV-jGCaMP7f

Plasmid

#104483

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

GENIE Project, Douglas Kim

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7f

Use

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

Plasmid

#64216

Purpose

Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA targeting ROSA26-1

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLSSmOrange-mKate2 caspase-3 biosensor

Plasmid

#37132

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange-DEVD-mKate2

Use

Tags

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2521 - 2560 of 4065 results

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