163,584 results
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pLIX403_Capture1_APOBEC_HA_P2A_mRuby
Plasmid#183901PurposeInducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTADepositorTypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsCapture1_APOBEC_HA_P2A_mRubyExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-PAK1 T423E
Plasmid#12208DepositorAvailable SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcdna 3.1 DSG-3 Tension Sensor
Plasmid#182717Purposeexpresses DSG-3 TSmodDepositorInsertdesmoglein-3 (Dsg3 Mouse)
UseTagsExpressionMammalianMutationTSmod inserted between G726 and G727PromoterAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-Lag16-iCAM-1
Plasmid#205195PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM iCAM1 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBC-popTag-QtE-mScarlet
Plasmid#240101PurposeMammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.DepositorInsertEncapsulin
UseLentiviralTagsmScarlet and popZExpressionBacterial and MammalianMutationPromoterUBC promoterAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
INPP5D-eGFP WT
Plasmid#161998PurposeMammalian expression vector, containing a GFP tagged version of INPP5DDepositorAvailable SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
RAB14 WT
Plasmid#61493Purposeto overexpress wild type RAB14. Vector includes GFP driven by hUbC promoterDepositorAvailable SinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP
Plasmid#126706Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequenceDepositorAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE
Plasmid#101852PurposeDirect reprogramming of adult fibroblasts to neuronsDepositorInsertAscl1, Brn2
UseLentiviralTagsExpressionMutationPromoterU6, PGKAvailable SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc IRES neo
Plasmid#98294PurposeFor constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance geneDepositorInsertsFirefly luciferase
IRES
Neomycin resistance gene (NeoR)
UseLentiviral and LuciferaseTagsExpressionMutationPromoterAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-oG-WPRE-SV40pA
Plasmid#74291PurposepAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent mannerDepositorInsertoG (optimized Glycoprotein)
UseAAVTagsExpressionMammalianMutationchimeric glycoproteinPromoterCAGAvailable SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-NLS-TIR1_P2A_NES-TIR1
Plasmid#117699PurposeExpression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNADepositorInsertsTRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1
UseTagsHA tag, Myc-NES, and NLSExpressionMammalianMutationcodon optimized for murine expressionPromoterNone, 2A peptide from porcine teschovirus-1 polyp…Available SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_VSVG-SBP-EGFP
Plasmid#65300Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMPRA1
Plasmid#49349PurposeBasic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) librariesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
c-Jun
Plasmid#187902PurposeExpresses c-Jun in transfected cellsDepositorAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
SV40-eGFP-Z1
Plasmid#127638PurposeMinimal backbone plasmid for mammalian eGFP expression from SV40 promoter with zeocin/bleocin bacterial resistanceDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterSV40Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Arl13b-EGFP
Plasmid#234844PurposeA mammalian expression plasmid encoding Arl13b with EGFP at C-terminus.DepositorAvailable SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a dCas9-HA 2A Blast
Plasmid#102812PurposeLentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion proteinDepositorInsertdCas9-HA
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A (dCas9)PromoterEF1aAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9
Plasmid#175547PurposeCo-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertH2B-HaloTag7 and Tomm20-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-CANX
Plasmid#227279PurposeExpresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.DepositorInsertsgRNA Targeting C-terminus of CANX (CANX Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-Electra2
Plasmid#184930PurposeTubulin labeling with blue fluorescent protein Electra2DepositorInsertTUBA1B-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL4393 (pClpX_Eco)
Plasmid#200907PurposeExpresses human codon-optimized E. coli ClpX from a CMV promoter.DepositorInsertEcoClpX
UseTagsNLSExpressionMammalianMutationPromoterAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP SMG1 WT
Plasmid#199571PurposeExpresses WT SMG1 fused to GFPDepositorAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6KT-mini-Tn7T-Km
Plasmid#64969Purposemini-Tn7 base vector with transcriptional terminator and oriTDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVT100U-mtGFP
Plasmid#45054DepositorInsertmtGFP
UseTagsExpressionYeastMutationPromoterADHAvailable SinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Tet-OsTIR1(WT)-V5
Plasmid#158663PurposeTet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locusDepositorInsertTet-OsTIR1(WT) AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX9-pmCherryC1
Plasmid#27678DepositorAvailable SinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-CANX
Plasmid#227280PurposeDonor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)DepositorInsertCANX Homology Arms flanking a mStayGold Tag (CANX Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-Claudin-1
Plasmid#46387PurposeClaudin-1 promoter reporter assayDepositorAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5FRT/TO_HA-mAID-nanobody
Plasmid#117713PurposeExpression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)DepositorInsertmAID-nanobody
UseTagstriple HAExpressionMammalianMutationcodon optimized mAID for murine expressionPromoterCMVAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL3713 (pTnsC_Pse)
Plasmid#200900PurposeExpresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.DepositorInsertPseTnsC
UseTags3xNLSExpressionMammalianMutationPromoterAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)
Plasmid#160736PurposeSingle-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDV05.2_pEF1a-iRFP720_secreted
Plasmid#200409PurposeExpresses a secreted version of iRFP720 constitutively in mammalian cellsDepositorInsertiRFP720 (secreted)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4-mammalian-recoded_mCherry_pcDNA3.1
Plasmid#114595PurposeEncodes mCherry-tagged BcLOV4 for expression in mammalian cellsDepositorInsertBcLOV4
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-LSL-nlsGFP-shRNA
Plasmid#175584PurposeConditional express shRNA with control of LoxPDepositorTypeEmpty backboneUseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEFSAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRAdonor2
Plasmid#49353PurposeminP-luc2 donor plasmid for MPRA library constructionDepositorInsertluc2
UseLuciferaseTagsExpressionMutationPromoterminPAvailable SinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His hTFNG
Plasmid#67240PurposeExpresses N-His tagged nonglycosylated human serum transferrin in mammalian cellsDepositorInserthuman serum transferrin (TF Human)
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationAsn413 Asp, Asn611AspPromoterSV40Available SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pN1-RnKIF5C(1-560,Δ6)-Halo-Flag
Plasmid#188072PurposeMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.DepositorInsertrat KIF5C (Kif5c Rat)
UseTagsFlag and HaloExpressionMammalianMutationaa1-560, deletion of aa2-6PromoterCMVAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only
