170,816 results
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Plasmid#80480PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)DepositorUsePiggybac transposonExpressionMammalianMutationKlf4 [10-483]PromotertetOAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only
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AiP12109 - pAAV-AiE0410m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2109)
Plasmid#208157PurposeDirect-expressing SYFP2 in oligodendrocytes AAV VirusDepositorInsertSYFP2
UseAAVMutationNAPromoterBeta Globin minimal promoterAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
dClover2-C1
Plasmid#54577PurposeLocalization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.DepositorTypeEmpty backboneTagsdClover2ExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-FLEX-DTR-GFP
Plasmid#124364Purposeconditional expression of a diphtheria toxin receptor (DTR)–GFP fusion proteinDepositorHas ServiceAAV2InsertDTR-GFP
UseAAVTagsGFPPromoterChicken B-actinAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TALEN-L
Plasmid#59025PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-L
UseAAV and TALENTags3xFlag and NLSExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone
Plasmid#216152PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic exon and C-terminal FLAG
ExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SFNp-mRFP1
Plasmid#238473PurposeExpresses higher mRFP1 fluorescence when NFkB is more active. Cloned into pLenti backboneDepositorInsertSFNp-mRFP1
PromoterSFNpAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDonor MCS Rosa26
Plasmid#37200DepositorInsertsRosa26 left targeting arm
MCS
Rosa26 right targeting arm
UseGenomic targeting - zinc fingerAvailable SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TALEN-R
Plasmid#59026PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-R
UseAAV and TALENTags3xFlag and NLSExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mEGFP-kanMX6
Plasmid#105146PurposeYeast gene targetingDepositorInsertmEGFP-kanMX6
TagsmEGFPExpressionBacterial and YeastMutationA206KAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_TNIK
Plasmid#45276DepositorAvailable SinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPolI_SARS-CoV-2_NLuc
Plasmid#183953PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoterDepositorExpressionMammalianPromoterMammalian Pol IAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorInsertcAMPFIRE-L (cAMP sensor)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-tRFP
Plasmid#230938PurposeModified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACSDepositorTypeEmpty backboneUseLentiviralTagsTagRFPAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
CLYBL_Bxb1-GA_LP_Dual_TC
Plasmid#229776PurposeCLYBL targeting construct with the Bxb1-GA landing pad cassette.DepositorInsertFRT-mScarlet-attP(GA)-PuroR-F3
UseSynthetic BiologyExpressionMammalianPromoterpGKAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRE-dCas9-KRAB-MeCP2-BSD
Plasmid#140690PurposeInducible knockdown of gene expression in human cells for pooled scRNA-seq experimentsDepositorInsertCas9m4-KRAB-MeCP2
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003 S49C
Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRA_p2_TCRB
Plasmid#131033PurposeExpresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptideDepositorArticleAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-hATG2A
Plasmid#114462PurposeExpresses human ATG2A with GFP in mammalian cellsDepositorInserthuman ATG2A (ATG2A Human)
UseRetroviralTagsGFPExpressionMammalianMutationP656R (please see depositors comment below)PromoterCMVAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKC105_pLenti_TetON_PABPC1_INT
Plasmid#242110PurposeTet- or Dox-inducible expression of PABPC1 fused to INT (human PARP4 fragment)DepositorInsertPAB4L, PARP4 fragment (PABPC1 Human)
UseLentiviralMutationPABPC1 aa 1-370, PARP4 aa 1562-1724PromoterTRE3GAvailable SinceDec. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3
Plasmid#172923PurposeFor enriched expression of jGCaMP8f in axons with cytosolic expression of mRuby3DepositorHas ServiceAAV1 and AAV9Insertaxon-jGCaMP8f-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domain (N terminal on insert…ExpressionMammalianPromoterhSynapsin1Available SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
scAAV-hSyn-Cre
Plasmid#138253PurposeA self-complementary AAV vector expresses Cre recombinase from hSyn promoter.DepositorInsertCre recombinase
UseAAV and Cre/LoxExpressionMammalianPromoterhSynAvailable SinceJuly 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Cre
Plasmid#34564PurposeRetroviral expression of CreDepositorInsertCre
UseCre/Lox and RetroviralExpressionMammalianPromoter5'LTRAvailable SinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3-mRFP
Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
TagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR_LG lenti-(empty)-del-CTS
Plasmid#112895PurposeNon-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid librariesDepositorArticleTypeEmpty backboneUseLentiviralPromoternoneAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G_SS-HA-V5-LaccID-CD4 TM_Puro
Plasmid#234047Purposeexpresses LaccID on the mammalian cell surface, dox-inducible lentiviral vectorDepositorInsertLaccID-CD4TM
UseLentiviralExpressionMammalianPromoterTRE3GAvailable SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-N108Q
Plasmid#194157PurposeMammalian expression of ABE8e-N108QDepositorInsertTadA-8e (N108Q)-nSpCas9
UseCRISPRTags6*HisExpressionMammalianAvailable SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu8
Plasmid#98297PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd3
Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EGFP-P4M-SidMx2
Plasmid#136997PurposeLentiviral EGFP lipid sensor for PI(4)PDepositorInsertEGFP-P4M-SidMx2
UseLentiviralTagsEGFPPromoterEF1 alphaAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA
Plasmid#61593PurposeA single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.DepositorInsertshSaCas9
Chimeric guide for SaCas9
UseAAV and CRISPRTagsHA, NLS, and OLLAS tagExpressionMammalianMutationK175R and K736R (deUb mutations)Available SinceFeb. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-mE1
Plasmid#32534DepositorAvailable SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIB184-Km
Plasmid#90195PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
ER-B
Plasmid#209870PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddGFP B
TagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
2Flag-pDEST-N
Plasmid#118371PurposeGateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2FlagExpressionMammalianAvailable SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-N1
Plasmid#54614PurposeLocalization: N1 Cloning Vector, Excitation: 559, Emission: 600DepositorTypeEmpty backboneTagsmRuby2ExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pATetO 6XHis-Phusion
Plasmid#206210PurposeInducible expression of DNA polymeraseDepositorInsertPhusion DNA polymerase
ExpressionBacterialAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLHKII-pGFPN3
Plasmid#21920DepositorInsertFull length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)
TagsGFPExpressionMammalianMutationThis is the full length human HKII cDNA sequence …Available SinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only