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Showing: 301 - 350 of 11375 results

  1. 3xmTurquoise2_UtrCH

    Plasmid
    #112963
    Purpose
    In vivo visualization of F-actin (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    UtrCH
    Use
    Tags
    3x mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112963-plasmid-map-sequence-id-220458

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  2. pRS428

    Plasmid
    #11257
    Depositor
    Fred Cross
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    11257-plasmid-map-sequence-id-6931

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  4. CytERM_3xmTurquoise2

    Plasmid
    #112958
    Purpose
    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
    Depositor
    Dorus Gadella
    Insert
    CytERM
    Use
    Tags
    3x mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112958-plasmid-map-sequence-id-220333

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  8. pCAG-mTHC

    Plasmid
    #112620
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112620-plasmid-map-sequence-id-226488

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  16. LUC2 (L/Sec/deltaP)

    Plasmid
    #112145
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U) and d…
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112145-plasmid-map-sequence-id-214904

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  21. pcDNA3-ΔN-RNMT

    Plasmid
    #112708
    Purpose
    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10
    Insert
    RNMT (RNMT Human)
    Use
    Tags
    FLAG and HIV Rev nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    deleted amino acids 2-120, mutated active-site D2…
    Promoter
    CMV
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112708-plasmid-map-sequence-id-217040

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  22. pAAVS hOPM

    Plasmid
    #112498
    Purpose
    Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in AAVS locus.
    Depositor
    Bruce Conklin
    Insert
    OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    112498-plasmid-map-sequence-id-232755

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  23. pGDP4 tet(A)

    Plasmid
    #112887
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    tet(A)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Sequence was codon optimized for E. coli K12 usin…
    Promoter
    Plac
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112887-plasmid-map-sequence-id-219894

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  29. pESR1-donor

    Plasmid
    #112337
    Purpose
    CRISPR donor plasmid to tag human transcription factor ESR1 with GFP
    Depositor
    Kevin White
    Insert
    ESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Unknown
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    112337-plasmid-map-sequence-id-241244

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  33. pORFE0011

    Plasmid
    #112075
    Purpose
    pcUBI promoter module Pro+5U
    Depositor
    Christian Chevalier
    Insert
    pcUBI promoter
    Use
    Tags
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112075-plasmid-map-sequence-id-217625

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  34. pVHC_PGKneoLox2DTA.2

    Plasmid
    #112622
    Purpose
    H2BVenus_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Venus
    Expression
    Mutation
    Promoter
    No Promoter
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112622-plasmid-map-sequence-id-222021

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  35. pANY6

    Plasmid
    #112198
    Purpose
    This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.
    Depositor
    Yingfeng An
    Article
    Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61
    Type
    Empty backbone
    Use
    Tags
    ELP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    112198-plasmid-map-sequence-id-218116

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  41. pESR1.1.0-gDNA

    Plasmid
    #112433
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1
    Depositor
    Kevin White
    Insert
    ESR1 (ESR1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112433-plasmid-map-sequence-id-230374

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  42. pcDNA3.1-hRIPK1-Flag-S320A

    Plasmid
    #112491
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S320A
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112491-plasmid-map-sequence-id-214534

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  44. pARNTL-donor

    Plasmid
    #112384
    Purpose
    CRISPR donor plasmid to tag human transcription factor ARNTL with GFP
    Depositor
    Kevin White
    Insert
    ARNTL homology arms flanking EGFP-IRES-Neo cassette (BMAL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112384-plasmid-map-sequence-id-229842

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  45. LUC45 (L/Sec/mutP)

    Plasmid
    #112148
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U) and SE…
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112148-plasmid-map-sequence-id-214874

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  46. pOP3SU

    Plasmid
    #112599
    Purpose
    Used for expression of protein in E. coli as N-terminal Sumo tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Sumo
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112599-plasmid-map-sequence-id-220209

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  49. pTFE3.1.0-gDNA

    Plasmid
    #112473
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3
    Depositor
    Kevin White
    Insert
    TFE3 (TFE3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112473-plasmid-map-sequence-id-230504

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Showing: 301 - 350 of 11375 results