163,580 results

1. Active Wnt10B-V5

   Plasmid

   #43823

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   WNT10B (WNT10B Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC65

   Plasmid

   #118865

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease, mGFP

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgPten#2/Cre

   Plasmid

   #173646

   Purpose

   Expresses a Pten-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAB744

   Plasmid

   #176579

   Purpose

   Expressing the whole n-butanol pathway.

   Depositor

   Arpita Bose

   Article

   Bai et al Commun Biol. 2021 Nov 3;4(1):1257. doi:

   Insert

   phaJ, ter, adhE2, phaA, phaB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNuc-TrxRFP1

   Plasmid

   #98998

   Purpose

   mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

   Depositor

   Huiwang Ai

   Article

   Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

   Insert

   TrxRFP1 and nuclear localization sequence
   

   Use

   Tags

   His-tag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBsVchCAST-pyrE

   Plasmid

   #203813

   Purpose

   Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

   Depositor

   Sheng Yang

   Article

   Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pgRNA-CGG

   Plasmid

   #108248

   Purpose

   Lentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   sgRNA targeting GGCGGCGGCGGCGGCGGCGG
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-FLEX-Chrimson-TdTomato

   Plasmid

   #209202

   Purpose

   Cre-dependent expression of mCherry-tagged Chrimson; Optogenetics

   Depositor

   Larry Zweifel

   Article

   Soden et al Nat Neurosci. 2020 Aug;23(8):968-980. do

   Insert

   Chrimson
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. shPBRM1#32-Tet-pLKO-puro

   Plasmid

   #107399

   Purpose

   Lentiviral expression of shRNA targeting PBRM

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   shRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMALp2x-CARP-1

    Plasmid

    #41727

    Depositor

    Koji Okamoto

    Article

    Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    clam ADP-ribosylating protein-1
    

    Use

    Bacterial subcloning

    Tags

    Expression

    Mutation

    deleted 5'-UTR nucleotides 1-64

    Promoter

    pMB1

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

12. LifeAct-EGFP-APEX2 in pCSDEST2

    Plasmid

    #170523

    Purpose

    Vector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTE

    Depositor

    Rob Parton

    Article

    Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

    Insert

    Cavin4 (Cavin4 Mouse)
    

    Use

    Tags

    APEX2-P2A-mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINK-OTUD4 (OTU, aa 22-196)

    Plasmid

    #61413

    Purpose

    Expresses human OTUD4 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-21 and aa 197-1114.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCerulean-Nucleus-12

    Plasmid

    #54556

    Purpose

    Localization: Nucleus, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mCerulean-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

15. TFORF0266

    Plasmid

    #142578

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF.DEST51-mVenus

    Plasmid

    #154899

    Purpose

    Expresses a fluorescent protein, mVenus

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-ERBB3-v2

    Plasmid

    #154894

    Purpose

    Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Tags

    Venus fragment 2 (v2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. SENP2M497A

    Plasmid

    #61088

    Purpose

    cleave the SUMO off when use PETSUMO vector for express fusion protein

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    SENP2M497A catalytic domain (SENP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M497A; Catalytic domain is residues 364-589

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pQTEV-UFD1L

    Plasmid

    #31560

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    ubiquitin fusion degradation 1-like (UFD1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJUMP47-2A(sfGFP)

    Plasmid

    #126991

    Purpose

    Level 2 vector. sfGFP reporter. Origin pSC101 (low copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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