165,688 results

1. Anti-Nav1.2 Na+ channel [K69/3R]

   Plasmid

   #206520

   Purpose

   Mammalian Expression Plasmid of anti-Nav1.2 Na+ channel (Rat). Derived from hybridoma K69/3.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Nav1.2 Na+ channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Scn2a Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-Kv2.1 K+ channel (external) [K39/25R-2b]

   Plasmid

   #206625

   Purpose

   Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (external) (Human). Derived from hybridoma K39/25-2b.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Kv2.1 K+ channel (external) (Homo sapiens) recombinant Mouse monoclonal antibody (KCNB1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Diffusive 80kDa

   Plasmid

   #201384

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Insert

   EGFP-8PrA
   

   Expression

   Mammalian

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNHA-C1-HsLSm1_J

   Plasmid

   #146658

   Purpose

   Mammalian Expression of HsLSm1

   Depositor

   Elisa Izaurralde

   Article

   Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

   Insert

   HsLSm1 (LSM1 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-CTLA4-COMP5AP-AviTag-9xHis

   Plasmid

   #157074

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CTLA4 (CTLA4 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIG-719 CTLA4-GFP R70W

   Plasmid

   #186124

   Purpose

   CTLA4 gene knockin with mutation

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   CRISPR

   Mutation

   R70W

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TetO-FUW-MCS-VP64_C-term

   Plasmid

   #136887

   Purpose

   Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the VP64 activator domain and a STOP codon

   Depositor

   Moritz Mall

   Article

   Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

   Insert

   VP64 activator domain
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. hKCNB1 L211P

   Plasmid

   #124487

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed leucine 211 to proline

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. hKCNB1 G381R

   Plasmid

   #124490

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed glycine at 381 to arginine

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. hKCNB1 F416L

    Plasmid

    #124491

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed phenyalanine 416 to leucine

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. hC73M-HA

    Plasmid

    #111528

    Purpose

    Expresses human KCNB1 C73M in mammalian cells

    Depositor

    Federico Sesti

    Insert

    human C73M-Kv2.1 + HA (KCNB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C73M

    Promoter

    CMV

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_CHD2_p.P195L

    Plasmid

    #81463

    Purpose

    Gateway Donor vector containing CHD2 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CHD2 (CHD2 Human)
    

    Use

    Gateway entry vector

    Mutation

    P195L

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_CHD2_p.E287Q

    Plasmid

    #81467

    Purpose

    Gateway Donor vector containing , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CHD2 (CHD2 Human)
    

    Use

    Gateway entry vector

    Mutation

    E287Q

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_CHD2_p.E412K

    Plasmid

    #81469

    Purpose

    Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CHD2 (CHD2 Human)
    

    Use

    Gateway entry vector

    Mutation

    E412K

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFGC5941-PacI-ath-STTM832-5p

    Plasmid

    #84163

    Purpose

    target miRNA832-5p for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM832-5p
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFGC5941-PacI-ath-STTM832-3p

    Plasmid

    #84162

    Purpose

    target miRNA832-3p for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM832-3p
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PCMV-Tag4A-MMP8 (E138Q)

    Plasmid

    #29549

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    E138Q

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pSicoR human Dicer1

    Plasmid

    #14763

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Dicer shRNA (DICER1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-15F11-HA-Halo

    Plasmid

    #129592

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-3XHA-EGFP-OMP25

    Plasmid

    #83356

    Purpose

    For tagging mitochondria with HA epitopes (HA-MITO construct)

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XHA-EGFP-OMP25
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. VSVG-mCherry-RUSH

    Plasmid

    #203647

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    VSVG
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcmv3-IRG1-HA

    Plasmid

    #198181

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1)

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    HA tag is added to C-terminal of target protein

    Promoter

    CMV

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCRIS-PITChv2-dTAG-BSD (BRD4)

    Plasmid

    #91795

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-BSD
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pvPE-V4-Puro

    Plasmid

    #240290

    Purpose

    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene into mammalian cells for gene editing.

    Depositor

    Lusheng Huang

    Article

    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

    Insert

    pvPE-V4-Puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. Sirius/pRSETB

    Plasmid

    #51956

    Purpose

    bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

    Insert

    Sirius
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pREP-8xARE-GFP-SV40-BFP

    Plasmid

    #134910

    Purpose

    NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Inserts

    8xARE- minimal promoter - GFP
    TagBFP
    

    Expression

    Mammalian

    Promoter

    8xARE - minimal promoter and SV40

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-D1R

    Plasmid

    #89160

    Purpose

    Express vaccinia virus D1R in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D1R
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSicoR human Drosha1

    Plasmid

    #14766

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Drosha shRNA (DROSHA Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pcDNA3-EGFP-RhoA-Q63L

    Plasmid

    #12968

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA constitutively active (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBAD-bARGSer-AxeTxe

    Plasmid

    #192473

    Purpose

    Second-generation bacterial acoustic reporter gene derived from Serratia

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Inserts

    bARGSer
    AxeTxe
    

    Expression

    Bacterial

    Mutation

    the gene Ser39006_001280 was deleted from the clu…

    Promoter

    Native promoter from Enterococcus faecium and pBAD

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Aequorea victoria, Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLB

    Plasmid

    #11619

    Purpose

    pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

    Depositor

    Stephan Kissler

    Article

    Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG-D12L

    Plasmid

    #89161

    Purpose

    Express vaccinia virus D12L in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D12L
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP-N1-FLAG

    Plasmid

    #60360

    Purpose

    Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcs2-GFP-IRES-mCherry

    Plasmid

    #226098

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUC18R6KT-mini-Tn7T-Km

    Plasmid

    #64969

    Purpose

    mini-Tn7 base vector with transcriptional terminator and oriT

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid

    #229676

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Halo, 3xALFA

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBBR1MCS-2

    Plasmid

    #85168

    Purpose

    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

    Depositor

    Kenneth Peterson

    Article

    Kovach et al Gene. 1995 Dec 1;166(1):175-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Constitutive (derepressed P-lac)

    Available Since

    Nov. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pAAV-mCherry-flex-dtA

    Plasmid

    #58536

    Purpose

    Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

    Depositor

    Naoshige Uchida

    Article

    Wu et al Nature. 2014 May 15;509(7500):325-30. do

    Insert

    dtA (DIP_RS18250 )
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge