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173,051 results

Showing: 2961 - 3000 of 173051 results

  12. Membrane EGFP

    Plasmid
    #198056
    Purpose
    Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Insert
    LCK membrane EGFP (LCK Synthetic, Human, A. victoria)
    Use
    Sea urchin, mammal, zebrafish
    Tags
    EGFP
    Mutation
    Tandem repeat of N-terminal LCK membrane targetin…
    Promoter
    sp6
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    198056-plasmid-map-sequence-id-389155

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  19. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

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  20. mCherry-Mem

    Plasmid
    #55779
    Purpose
    This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCherry-Mem
    Tags
    The N-terminal 20 residues of neuromodulin are fu…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    55779-plasmid-map-sequence-id-121746

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  22. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid
    #102244
    Purpose
    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
    Depositor
    Jacob Corn
    Insert
    dCas9-HA-BFP-KRAB-NLS
    Use
    CRISPR and Lentiviral
    Tags
    HA-TagBFP-KRAB-NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102244-plasmid-map-sequence-id-197289

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  25. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid
    #106965
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106965-plasmid-map-sequence-id-207982

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  34. pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)

    Plasmid
    #72639
    Purpose
    Tol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
    Use
    Gateway Cloning
    Mutation
    Stop codon after mCherry- SEE DEPOSITOR COMMENTS …
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72639-plasmid-map-sequence-id-429021

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  35. FUGW-Arl13b-PR-EGFP-TurboID

    Plasmid
    #248194
    Purpose
    Lentiviral expression of truncated Arl13b containing the Proline-Rich domain at the C-terminus (cytosol localization)
    Depositor
    Xuecai Ge
    Article
    Liu et al Nat Commun. 2024 Apr 25;15(1):3365. doi:
    Insert
    The Arl13b noncilium targeting sequence-EGFP-TurboID (Arl13b Mouse)
    Use
    Lentiviral
    Tags
    EGFP and TurboID
    Expression
    Mammalian
    Mutation
    Truncated to only contain the PR domain
    Promoter
    hUBC
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    248194-plasmid-map-sequence-id-494577

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  36. FUGW-Arl13b-N+RVEP+PR-EGFP-TurboID

    Plasmid
    #248193
    Purpose
    Lentiviral expression of truncated Arl13b containing the N-terminal amphipathic helix, cilium targeting RVEP, and the Proline-Rich domain at the C-terminus (cilium localization)
    Depositor
    Xuecai Ge
    Article
    Liu et al Nat Commun. 2024 Apr 25;15(1):3365. doi:
    Insert
    The Arl13b cilium targeting sequence-EGFP-TurboID (Arl13b Mouse)
    Use
    Lentiviral
    Tags
    EGFP and TurboID
    Expression
    Mammalian
    Mutation
    Truncated to only contain N + RVEP + PR domains
    Promoter
    hUBC
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    248193-plasmid-map-sequence-id-494576

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  39. pRecT_2

    Plasmid
    #167546
    Purpose
    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Insert
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    Expression
    Bacterial
    Promoter
    IPTG
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167546-plasmid-map-sequence-id-332204

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Showing: 2961 - 3000 of 173051 results