161,641 results

1. lenti-sgRNA blast

   Plasmid

   #104993

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-eLACCO1.1

   Plasmid

   #168788

   Purpose

   Neuronal expression of fluorescent sensor for extracellular lactate

   Depositor

   Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLACCO1.1
   

   Use

   AAV

   Tags

   ER export and GPI

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4 [luc2P/Myc/Hygro] Vector

   Plasmid

   #236815

   Purpose

   Express Myc response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   Myc (MYC Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   minP

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCS Omomyc-Mer

   Plasmid

   #113170

   Purpose

   Expression of Omomer (tamoxifen inducible Omomyc) in mammalian cells

   Depositor

   Sergio Nasi

   Insert

   Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E57T, E64I, R70Q, R71N

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

5. TFORF2546

   Plasmid

   #144209

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CEBPA (CEBPA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-GFAP-eLACCO1.1

   Plasmid

   #168790

   Purpose

   Astrocyte expression of fluorescent sensor for extracellular lactate

   Depositor

   Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLacco1.1
   

   Use

   AAV

   Tags

   ER export and GPI

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. scFLARE2

   Plasmid

   #158700

   Purpose

   Neuronal expression of scFLARE2 component

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   scFLARE2
   

   Use

   AAV

   Tags

   HA, Flag, VP16

   Expression

   Mammalian

   Mutation

   Truncated form of TEV protease (220-242 amino aci…

   Promoter

   Syn

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXR001: EF1a-CasRx-2A-EGFP

   Plasmid

   #109049

   Purpose

   CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Insert

   CasRx
   

   Use

   CRISPR and Lentiviral

   Tags

   2A-eGFP, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-syn-jGCaMP8m-WPRE

   Plasmid

   #162375

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   Synapsin

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSin Helper

    Plasmid

    #127694

    Purpose

    Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Glycoproteins
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pcDNA-HDAC6.DC-FLAG

    Plasmid

    #30483

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Catalytically inactive mutant (H216/611A)

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE-BI-hGRAD

    Plasmid

    #207837

    Purpose

    Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    mCherry
    hFBXW11 (FBXW11 Human)
    VHH-GFP4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    215 amino acids of the full length protein

    Promoter

    TRE3G BI promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP264

    Plasmid

    #149334

    Purpose

    Bacterial expression of Biotin ligase BirA

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Biotin ligase BirA
    

    Use

    Tags

    His14 and bdNEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEFBOS_CreIRESpuro

    Plasmid

    #183812

    Purpose

    Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

    Depositor

    Ed Stanley

    Article

    Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

    Insert

    Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_MAX_WT_V5

    Plasmid

    #82944

    Purpose

    Gateway Donor vector containing MAX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    MAX (MAX Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 LIC cloning vector (6A)

    Plasmid

    #30124

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

    Plasmid

    #217306

    Purpose

    sgRNA mcherry + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6, Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-cdh1

    Plasmid

    #11596

    Depositor

    Marc Kirschner

    Article

    Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

    Insert

    cdh1 (FZR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lifeact-mCherry

    Plasmid

    #193300

    Purpose

    mammalian transient expression - expression of Lifeact-mCherry

    Depositor

    Klaus Hahn

    Article

    Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

    Insert

    Lifeact
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK793

    Plasmid

    #8827

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219V mutant
    

    Use

    Tags

    His, MBP (with TEV), and polyarginine

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability)

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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21. MSCVneo-HA-ER-Hoxb8

    Plasmid

    #222291

    Purpose

    The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

    Depositor

    Mark Kamps, David Sykes

    Article

    Wang et al Nat Methods. 2006 Apr;3(4):287-93.

    Insert

    ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The estrogen receptor hormone binding domain cont…

    Promoter

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Cbh_v5 AAV-ABE C-terminal

    Plasmid

    #137178

    Purpose

    AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE C-terminal; U6-protospacer
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNCS BFP

    Plasmid

    #91757

    Purpose

    Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

    Insert

    Blue Fluorescent Protein
    

    Use

    Tags

    Hexa-Histidine, Xpress epitope for detection with…

    Expression

    Bacterial

    Mutation

    GFP (Y66H)

    Promoter

    T7

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p4xmts-mScarlet3_N1

    Plasmid

    #189774

    Purpose

    Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    4xmts-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDest17_MAX

    Plasmid

    #234044

    Purpose

    Bacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage site

    Depositor

    Tanja Mittag

    Article

    Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

    Insert

    MAX (MAX Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHR CD19-mMegf10 CAR

    Plasmid

    #113013

    Purpose

    for production of lentivirus encoding the CD19 CAR-Pmegf10

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    CD19 CAR-P Megf10 intracellular domain (CD19, Megf10 Human, Mouse, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJRH-1179 U6-reci Gag-Cas9 v2

    Plasmid

    #201914

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-Cas9 v2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CMV-Mito4x-jRCaMP1b

    Plasmid

    #127873

    Purpose

    Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Red-shifted mitochondrial matrix Ca2+ indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSCV-Cas9-2A-GFP-sgRNA

    Plasmid

    #124889

    Purpose

    Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

    Depositor

    Harvey Lodish

    Article

    Li et al Haematologica. 2016 Jun;101(6):e216-9. d

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    2A-GFP

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAY5 (mAID-EGFP-NLS piggyBac)

    Plasmid

    #140532

    Purpose

    mAID-EGFP-NLS piggyBac

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR223-NTRK2

    Plasmid

    #23883

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NTRK2 (NTRK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKSE401

    Plasmid

    #62202

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-FlipCherry-T2A-GFP

    Plasmid

    #124436

    Purpose

    Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipCherry-T2A-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MYC_pLX307

    Plasmid

    #98363

    Purpose

    Lentiviral expression of MYC

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MSGV1-1D3-28Z.1-3 mut

    Plasmid

    #107227

    Purpose

    Anti-mouse CD19 CAR with CD3 ITAMs mutated

    Depositor

    Steven Rosenberg, James Kochenderfer

    Article

    Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

    Insert

    1D3-28Z. 1-3 mut
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    None

    Promoter

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HA-HIF2alpha-pcDNA3

    Plasmid

    #18950

    Depositor

    William Kaelin

    Article

    Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA-FLAG-SF2

    Plasmid

    #99021

    Purpose

    Mammalian expression of SF2

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHIV-Luc-ZsGreen

    Plasmid

    #39196

    Depositor

    Bryan Welm

    Inserts

    Firefly Luciferase Luc2P
    ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha and EF1-alpha

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge