163,584 results

1. pCAG-T7pol

   Plasmid

   #59926

   Purpose

   Expression vector for T7 RNA polymerase gene

   Depositor

   Ian Wickersham

   Insert

   T7 RNA polymerase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB-cLUC

   Plasmid

   #174051

   Purpose

   Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

   Depositor

   Alberto Macho

   Article

   Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCX137

   Plasmid

   #229212

   Purpose

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LAMP1-mRFP-FLAG

   Plasmid

   #34611

   Purpose

   3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

   Depositor

   David Sabatini

   Article

   Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   2xFlag and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBBR1k-GFPuv

   Plasmid

   #106382

   Purpose

   IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mpeg:lamp1b-mCherry-stop; cmlc2:GFP

   Plasmid

   #213739

   Purpose

   Expresses Lamp1b-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

   Depositor

   William Talbot

   Article

   Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

   Insert

   lamp1b-mCherry
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   mpeg1.1

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mPiezo1-IRES-eGFP

   Plasmid

   #80925

   Purpose

   expresses mouse Piezo1 with GFP

   Depositor

   Ardem Patapoutian

   Article

   Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

   Insert

   mouse Piezo1 CDS (Piezo1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIB

   Plasmid

   #80610

   Purpose

   pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGWB-nLUC

   Plasmid

   #174050

   Purpose

   Gateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assay

   Depositor

   Alberto Macho

   Article

   Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   N-terminal luciferase

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pD4sE_SC12

    Plasmid

    #219721

    Purpose

    DV4 stable E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV4 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pD1sE_SC12

    Plasmid

    #219718

    Purpose

    DV1 E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV1 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…

    Promoter

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pttq18

    Plasmid

    #69122

    Purpose

    regulated expression of genes in Escherichia coli

    Depositor

    Michael Stark

    Article

    Stark et al Gene. 1987;51(2-3):255-67.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR- flag-Lag16-Ecad

    Plasmid

    #205197

    Purpose

    This vector encodes of the synCAM (Ecad) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM Ecad and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pD2sE_SC12

    Plasmid

    #219719

    Purpose

    DV2 stable E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV2 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, T280P, S29K, T33V, A2…

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. enAsABE8e

    Plasmid

    #138506

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dAsCas12a (En)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHSE901

    Plasmid

    #91708

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcsII-pb5-flag

    Plasmid

    #86769

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    proteasome beta subunit 5 (PSMB5 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDD120

    Plasmid

    #119879

    Purpose

    Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_Che9c60 and Che9c61
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

    Plasmid

    #216694

    Purpose

    Synchronize trafficking of GPI-AP from the ER (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    SBP-mApple-FIREtag-GPI
    

    Use

    Tags

    FIREtag, IL2 signal sequence, Streptavidin Bindin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV6M-Pak1 K299R

    Plasmid

    #12210

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Catalytically inactive Pak1: K299R

    Promoter

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAG1344 EGFP-FIREtag

    Plasmid

    #205165

    Purpose

    Expresses EGFP-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    EGFP-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCdrA-gfpC

    Plasmid

    #111614

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV_TRET_hNgn2_UBC_Puro

    Plasmid

    #61474

    Purpose

    3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn2 (NEUROG2 Human)
    Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. nuclear cpVenus control

    Plasmid

    #186790

    Purpose

    Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear cpVenus control
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1_AGIA-AirID

    Plasmid

    #182210

    Purpose

    Express AirID in mammalian cells.

    Depositor

    Shogo Nakano, Sohei Ito, Akira Nozawa, Tatsuya Sawasaki

    Article

    Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

    Insert

    AGIA-AirID
    

    Use

    Tags

    AGIA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-HA-Tuba

    Plasmid

    #22214

    Depositor

    Pietro De Camilli

    Article

    Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

    Insert

    Tuba (DNMBP Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

29. pRC0940 (circRNA entry vector)

    Plasmid

    #189967

    Purpose

    Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR- flag-Lag16-JAM-B

    Plasmid

    #205200

    Purpose

    This vector encodes of the synCAM (JAM-B) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM JAM-B and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOsU3

    Plasmid

    #170132

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAV SYN flex PSAM4 GlyR IRES EGFP

    Plasmid

    #119741

    Purpose

    Cre-dependent chemogenetic inhibitor expression

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5 and AAV9

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Sumo3 PolQM1

    Plasmid

    #78462

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; Q2513R (please see depositor commen…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

34. c-Fos

    Plasmid

    #187905

    Purpose

    Expresses c-Fos in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    cFos (FOS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMi(3xP3-EGFP)

    Plasmid

    #102540

    Purpose

    Transgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP marker

    Depositor

    Charalambos Savakis, Michalis Averof

    Article

    Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

    Insert

    Minos transpoable element with 3xP3-EGFP marker
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PJEBAN6

    Plasmid

    #203684

    Purpose

    Expresses red fluorescent marker DsRed-Express in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    DsRed-Express
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-iCre

    Plasmid

    #89573

    Purpose

    Expresses iCre under pCAG

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iCre
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSL3617 (pCRISPR(BbsI)_Pse)

    Plasmid

    #200902

    Purpose

    Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCRISPR (entry)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

    Plasmid

    #75470

    Purpose

    Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Reversed ChR2(H134R)-mCherry
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCIP-AMPKa1_WT

    Plasmid

    #79010

    Purpose

    For mammalian expression of WT AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGs

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge