164,223 results

1. pSAM_Ec

   Plasmid

   #102939

   Purpose

   Transposon mutagenesis and Tn-seq

   Depositor

   Matthew Mulvey

   Article

   Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

   Insert

   mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. phCMV-GALV-MTR

   Plasmid

   #163612

   Purpose

   Retro and Lentiviral transduction of primary human germinal center B cells

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Insert

   GaLV MTR envelope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. aeBlue chromoprotein

   Plasmid

   #117846

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, aeBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ14901

   Plasmid

   #239276

   Purpose

   Expresses gG1 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

   Depositor

   Stanley Qi

   Article

   Han et al Nature. 2025 Jul;643(8070):241-251. doi:

   Insert

   PspCas13b-hGAPDH-gRNA1 (GAPDH Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pESF140_HisSpy_DDB1∆B

   Plasmid

   #124213

   Purpose

   Insect cell expression vector for His6, Spy tagged hsDDB1∆B

   Depositor

   Eric Fischer

   Article

   Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

   Insert

   DNA damage-binding protein 1 (DDB1 Human)
   

   Use

   Tags

   His6-Spy

   Expression

   Insect

   Mutation

   internal deletion of BPB domain to aid crystalliz…

   Promoter

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST14-SpyCatcher002

   Plasmid

   #102827

   Purpose

   Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEVOL-pBpF

   Plasmid

   #31190

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.

   Depositor

   Peter Schultz

   Article

   Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

   Insert

   M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32G, E107P, D158T, I159S

   Promoter

   Available Since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3-EF1aH N90-beta-catenin

   Plasmid

   #86499

   Purpose

   Mammalian expression of Myc tagged N90 beta-catenin

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-90; constitutively acti…

   Promoter

   EF1a

   Available Since

   Feb. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCOLA-Gent-EM7-Erv1p-DsbC

   Plasmid

   #202485

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Escherichia coli, Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   EM7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ABE8e-Cas9NG

    Plasmid

    #203328

    Purpose

    Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    ABE8e-Cas9NG
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_LacZ

    Plasmid

    #25893

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    LacZ
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE_puro

    Plasmid

    #118692

    Purpose

    Empty vector to express overexpression cDNA

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti PGK V5-LUC Puro (w543-1)

    Plasmid

    #19360

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAJ075_hsCRBN

    Plasmid

    #124214

    Purpose

    Insect cell expression vector for His6 tagged hsCRBN

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    Cereblon (CRBN Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 7xE-Box::Renilla

    Plasmid

    #124532

    Purpose

    c-Myc Renilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    c-Myc Renilla reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Thy1.1-G5

    Plasmid

    #234839

    Purpose

    pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

    Depositor

    Gabriel Victora

    Article

    Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

    Insert

    Thy1.1 (Thy1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-P2A-5xGly

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-HSF1

    Plasmid

    #32537

    Depositor

    Stuart Calderwood

    Article

    Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    CMV

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTriEx-HTNC

    Plasmid

    #13763

    Purpose

    For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

    Depositor

    Klaus Rajewsky

    Article

    Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

    Insert

    His-TAT-NLS-Cre
    

    Use

    Baculovirus

    Tags

    His Tag, NLS, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    p10 promoter

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7 Promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. STAT3-GFP

    Plasmid

    #110495

    Purpose

    reporter of STAT3 signaling activity

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    4 repeats of STAT3 binding site M67 at GFP promoter region
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lenti-CMV-MLH1dn-IRES-puroR

    Plasmid

    #207354

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    MutL homolog 1 (MLH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Δ754-756 dominant negative mutant

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX037

    Plasmid

    #167156

    Purpose

    MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    caffeic acid cycle genes
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pNOS, p35s

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSP2-96-merTetO-EFS-BLaR

    Plasmid

    #118713

    Purpose

    Contains an optimized 96-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSIH1-puro-control shRNA

    Plasmid

    #26597

    Purpose

    Lentiviral control shRNA vector with puromycin selection.

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NW50

    Plasmid

    #133445

    Purpose

    Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW50
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid

    #174665

    Purpose

    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

    Depositor

    Eli Bar

    Inserts

    LUC2
    mCherry:T2A:Hygro
    

    Use

    Lentiviral and Luciferase

    Tags

    mCherry and hygromycin dual reporter gene linked …

    Expression

    Mammalian

    Mutation

    Promoter

    Human cytomegalovirus immediate early enhancer/pr…

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hSTARR-seq_SCP1 vector_blocking 4

    Plasmid

    #99319

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pVoPo-mCh

    Plasmid

    #187841

    Purpose

    Constitutive mCherry-expression vector for Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pICE-EGFP-FLAG-PLIN3

    Plasmid

    #161918

    Purpose

    Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Perilipin-3 (PLIN3 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. eeBxb1_IVT

    Plasmid

    #222342

    Purpose

    Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7 (inactivated)

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

    Plasmid

    #230988

    Purpose

    Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Cre-dependent, membrane-localized optopatch for neuronal dendrites
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pH2B-Electra2

    Plasmid

    #179479

    Purpose

    Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    H2B-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLKO.1-TetON-Puro-HIF1a 0819

    Plasmid

    #118704

    Purpose

    Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    shRNA against human HIF1a
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. dCas9-KRAB

    Plasmid

    #110820

    Purpose

    Conventional dCas9 repressor-dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHR CD19-FcGamma CAR

    Plasmid

    #113014

    Purpose

    for production of lentivirus encoding the CD19 CAR-Pfcgamma

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    CD19 CAR-P intracellular GFP (CD19 Mouse, Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBAD-mGreenLantern

    Plasmid

    #161913

    Purpose

    mGreenLantern fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEF-BFP

    Plasmid

    #138272

    Purpose

    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    BFP H66 PAM
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H66, PAM motif

    Promoter

    Ef1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-U6-sasgRNACMV- mCherry-WPREpA

    Plasmid

    #217015

    Purpose

    S. aureus sgRNAs backbone along with mCherry expression

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAVS1 SA-2A-puro-pA donor

    Plasmid

    #22075

    Purpose

    AAV zinc finger donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    SA-Puro
    

    Use

    Zfn donor

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge