166,201 results

1. pMOS023: Peredox NADH/NAD+ sensor (cytosolic)

   Plasmid

   #163060

   Purpose

   Peredox NADH/NAD+ sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Peredox
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_TetON_mCherry_P2AT2A_GFP-nb

   Plasmid

   #238239

   Purpose

   For integration of mCherry_P2AT2A_GFP-nb

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb
   

   Expression

   Mammalian

   Available Since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_ENVLPE+

   Plasmid

   #232427

   Purpose

   Expression plasmid to produce ENVLPE+ particles

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   ENVLPE+
   

   Expression

   Mammalian

   Promoter

   2xCMV

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSP1E3D1

   Plasmid

   #20066

   Purpose

   Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

   Insert

   MSP1E3D1
   

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeats of …

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMuLE_ENTR_12GLI-FLuc_R4-R3

   Plasmid

   #113712

   Purpose

   Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Insert

   12GLI-FLuc
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE30-His-PheRS

   Plasmid

   #124116

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

   Plasmid

   #175574

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Peroxisomes-2

   Plasmid

   #54520

   Purpose

   Localization: Peroxisomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   mCherry-Peroxisomes
   

   Tags

   Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEM415-ldhL-mRFP1

   Plasmid

   #99842

   Purpose

   Expression of RFP in Lactobacillus

   Depositor

   Sujin Bao

   Article

   Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

   Insert

   monomeric red fluorescent protein
   

   Tags

   FLAG/Xpress

   Expression

   Bacterial

   Promoter

   ldhl

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS

    Plasmid

    #238243

    Purpose

    For integration of mCherry_P2AT2A_GFP-nb-2xKS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-2xKS
    

    Expression

    Mammalian

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-GGamma1-GFP2

    Plasmid

    #140989

    Purpose

    Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma1-GFP2 (GNGT1 Human)
    

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti Puro HA-Ubiquitin-WT

    Plasmid

    #236289

    Purpose

    Lentiviral vector expressing HA-tagged wild type ubiquitin

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Ubiquitin (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    Single ubiquitin coding sequence (full length UBB…

    Promoter

    CMV

    Available Since

    April 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. MSCV Luciferase PGK-hygro

    Plasmid

    #18782

    Depositor

    Scott Lowe

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase and Retroviral

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid

    #140981

    Purpose

    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasL-RLuc8 (GNAS Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMVR8.74

    Plasmid

    #22036

    Purpose

    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Insert

    gag pol tat rev
    

    Expression

    Mammalian

    Available Since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV hSyn-fDIO-taCasp3-TEVp

    Plasmid

    #207997

    Purpose

    Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase

    Depositor

    Kazunari Miyamichi

    Article

    Goto et al Neuron. 2025 Feb 17:S0896-6273(25)00041-

    Has Service

    AAV5

    Insert

    taCasp3-TEVp
    

    Use

    AAV

    Promoter

    hSyn promoter

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dual-sgRNA_hU6-mU6

    Plasmid

    #154194

    Purpose

    Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEM705 Gs

    Plasmid

    #109350

    Purpose

    Wildtype Gαs

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    GαS (GNAS Human)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDNA2.0 6H-TEV-KRAS

    Plasmid

    #111849

    Purpose

    Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS (KRAS Human)
    

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDT305

    Plasmid

    #128801

    Purpose

    Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    

    Use

    CRISPR and Lentiviral

    Promoter

    hUBC promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pShuttle-CMV

    Plasmid

    #16403

    Purpose

    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSEVA451-Cpf1

    Plasmid

    #192362

    Purpose

    CRISPR-based vector for genome editing

    Depositor

    Juana María Navarro Llorens

    Article

    Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

    Insert

    Cas12a, gRNA scaffold
    

    Use

    CRISPR

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro

    Plasmid

    #231972

    Purpose

    Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSIN4-EF1a-ETV2-IRES-Puro

    Plasmid

    #61061

    Purpose

    lentiviral vector for ETV2

    Depositor

    Igor Slukvin

    Article

    Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

    Insert

    ETV2 (ETV2 Human)
    

    Use

    Lentiviral

    Promoter

    EF1alpha

    Available Since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAB150

    Plasmid

    #101661

    Purpose

    Opto-T7RNAP*(563)

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(563)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET15b-AavLEA1

    Plasmid

    #53093

    Purpose

    AavLEA1 expression (in BL21 DE3 E. coli strain)

    Depositor

    Claude Férec

    Article

    Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

    Insert

    AavLEA1
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. phU6-gRNA

    Plasmid

    #53188

    Purpose

    Expresses the S. pyogenes sgRNA from the human U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBABE-zeo largeTgenomic

    Plasmid

    #1778

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T and small T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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30. AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

    Plasmid

    #163476

    Purpose

    Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

    Depositor

    Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    iCre
    

    Use

    AAV

    Promoter

    Beta Globin minimal promoter

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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31. pcDNA3.1-GGamma13-GFP2

    Plasmid

    #140992

    Purpose

    Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma13-GFP2 (GNG13 Human)
    

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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32. tetO-TBX20

    Plasmid

    #170705

    Purpose

    doxycycline-inducible overexpression of TBX20

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    TBX20 (TBX20 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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33. pTW335h3

    Plasmid

    #237326

    Purpose

    expresses O. sativa RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Article

    Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

    Inserts

    O. sativa RbcL
    O. sativa RbcS-6xHis
    

    Tags

    6xHis and None

    Expression

    Bacterial

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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34. pTW386c

    Plasmid

    #237321

    Purpose

    expresses O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2

    Depositor

    Tina Wang

    Article

    Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

    Insert

    O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2
    

    Expression

    Bacterial

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid

    #140975

    Purpose

    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai3-RLuc8 (GNAI3 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a-MH6-uvsY

    Plasmid

    #163914

    Purpose

    Expresses uvsY for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsY (uvsY Enterobacteria phage T4)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SNAP-Sec61B

    Plasmid

    #141152

    Purpose

    Fluorescent labeling of ER membrane in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    L79M on Sec61B

    Promoter

    CMV

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP-TDP-43

    Plasmid

    #190093

    Purpose

    Expresses TDP-43 in mammalian cells. Note: EGFP and TDP-43 are expressed in different reading frames.

    Depositor

    Bowen Liu

    Article

    Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

    Insert

    TAR DNA-Binding Protein 43 (TARDBP Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TetO-FUW-OSKM

    Plasmid

    #20321

    Purpose

    Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid

    #140976

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoA-RLuc8 (GNAO1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge