We narrowed to 6,346 results for: rel

1. pRD443

   Plasmid

   #168469

   Purpose

   For the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mScarlet-I-H-NSdbd-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRD444

   Plasmid

   #168470

   Purpose

   For the insertion of NLS-mNeonGreen-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mNeonGreen-H-NSdbd-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3HA-AMOT p130∆PPxY2

   Plasmid

   #166712

   Purpose

   expresses AMOT with inactivating mutation in second PPxY motif in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   Angiomotin p130 P262A P263A (AMOT Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   changed proline 262 to alanine, proline 263 to al…

   Promoter

   CMV promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3HA-AMOT p130∆PPxY3

   Plasmid

   #166713

   Purpose

   expresses AMOT with inactivating mutation in third PPxY motif in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   Angiomotin p130 P3072A P308A (AMOT Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   changed proline 307 to alanine, proline 308 to al…

   Promoter

   CMV promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K)

   Plasmid

   #171130

   Purpose

   expresses AMOT(E104K/E105K) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   Angiomotin p130 E104K/E105K (AMOT Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   changed glutamic acid residues 104 and 105 to lys…

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. human TLNRD1-4H pET151

   Plasmid

   #159386

   Purpose

   Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

   Depositor

   Ben Goult

   Article

   Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
   

   Use

   Tags

   his-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   TLNRD1 4-helix domain (residues 143-273)

   Promoter

   T7

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mWasabi-ADAP1

   Plasmid

   #163906

   Purpose

   Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Use

   Tags

   mWasabi fluorescent tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP N1 AURKA deltaNter

   Plasmid

   #157753

   Purpose

   Expression of AURKA without Nter, fused to GFP in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV_Donor/Reporter-COL4A3

   Plasmid

   #130279

   Purpose

   COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   mCherry-eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV_COL4A3_self-cleavingCas9

    Plasmid

    #130281

    Purpose

    Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    spCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV_COL4A5_self-cleavingCas9

    Plasmid

    #130282

    Purpose

    Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    spCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSECC-sgSIK1-B

    Plasmid

    #138666

    Purpose

    Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSECC-sgSIK3-A

    Plasmid

    #138672

    Purpose

    Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSECC-sgSIK3-C

    Plasmid

    #138674

    Purpose

    Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSECC-sgSIK1-A

    Plasmid

    #138665

    Purpose

    Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSECC-sgSIK1-D

    Plasmid

    #138668

    Purpose

    Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSECC-sgSIK3-B

    Plasmid

    #138673

    Purpose

    Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSECC-sgSIK1-E

    Plasmid

    #138669

    Purpose

    Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSECC-sgSIK3-D

    Plasmid

    #138675

    Purpose

    Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-Blast-sgSIK1-D

    Plasmid

    #138680

    Purpose

    Expresses a mouse SIK1-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-Blast-sgSIK1-B

    Plasmid

    #138679

    Purpose

    Expresses a mouse SIK1-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pIE-N-hα2M-H6

    Plasmid

    #128493

    Purpose

    Expresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (S24-E908) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    Promoter

    IE1

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pS6-N-hα2M-H6

    Plasmid

    #128496

    Purpose

    Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (G27-A788) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn-NOS-IN133.3xFLAG-WPRE

    Plasmid

    #127869

    Purpose

    pAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1 (Nos1 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG

    Expression

    Mutation

    Amino acids 1-133 only

    Promoter

    hSyn

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. DLG3_PDZ_2

    Plasmid

    #103947

    Purpose

    Protein expression and purification of NE-DLG PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG3 (DLG3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MAGI1_PDZ_1

    Plasmid

    #103898

    Purpose

    Protein expression and purification of MAGI1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MAGI1_PDZ_4

    Plasmid

    #103901

    Purpose

    Protein expression and purification of MAGI1 PDZ4 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA4-Tet-on-vsv-Incenp d543-746iPRC1_279-482-GFP

    Plasmid

    #108500

    Purpose

    inducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746iPRC1 304-509 (PRC1 Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha Helix, insert PRC1 microtubule domain

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCR3-vsv-Incenp 8KQ

    Plasmid

    #108501

    Purpose

    expression of vsv-INCENP 8KQ

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5-FRT-TO-vsv-Incenp d543-746iPRC1_279-482-GFP

    Plasmid

    #108504

    Purpose

    inducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746iPRC1 304-509 (PRC1 Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha Helix, insert PRC1 microtubule domain

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHDT2:GUS

    Plasmid

    #108444

    Purpose

    Arabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in roots

    Depositor

    Ton Bisseling

    Article

    Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

    Insert

    HDT2 promoter (HD2B Mustard Weed)
    

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    HDT2

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHDT2:HDT2-GFP

    Plasmid

    #108566

    Purpose

    Arabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in roots

    Depositor

    Ton Bisseling

    Article

    Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

    Insert

    HDT2 (HD2B Mustard Weed)
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    HDT2

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PA-mCherry-miniSOG-Keratin-17

    Plasmid

    #57792

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

34. PA-mCherry-miniSOG-Alpha-Actinin-19

    Plasmid

    #57782

    Purpose

    Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

35. PA-mCherry-miniSOG-EB3-7

    Plasmid

    #57788

    Purpose

    Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

36. tdminiSOG-Actin-7

    Plasmid

    #57803

    Purpose

    Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    tdminiSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. mCherry-miniSOG-Vimentin-7

    Plasmid

    #55099

    Purpose

    Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    NM_003380.3 (VIM Human)
    

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

38. mCherry-miniSOG-Keratin-17

    Plasmid

    #55091

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pGEX-5x3-hMeCP2-delN256-G273X

    Plasmid

    #48090

    Purpose

    Bacterial expression of GST tagged AT-Hook2-G273X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted the N-terminal 256 codons, expresses AT-H…

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pGEX-5x3-hMeCP2-delN256-R270X

    Plasmid

    #48089

    Purpose

    Bacterial expression of GST tagged AT-Hook2-R270X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted the N-terminal 256 codons, expresses AT-H…

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

41. pCMX-Gal4-hMeCP2-G273X-R111G

    Plasmid

    #48087

    Purpose

    Mammalian expression of Gal4 tagged MeCP2-G273X-R111G

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    R111G mutant MeCP2, expresses AT-hook G273X

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

42. pX68

    Plasmid

    #46857

    Purpose

    OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

    Depositor

    Monica Hollstein

    Article

    Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

    Insert

    attB-Puro-2A and Hupki p53 A138V (TP53 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    p53 A138V

    Promoter

    Available since

    Aug. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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43. pCMV-Tag4A-MMP8 (E87D)

    Plasmid

    #29547

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    E87D

    Promoter

    Available since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

44. PCMV-Tag4A-MMP8 (E138Q)

    Plasmid

    #29549

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    E138Q

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

45. pMS2M-PH-gagpol-D64V

    Plasmid

    #166031

    Purpose

    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

    Insert

    MS2-Gag-Pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D64V mutation within the integrase within Pol

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLVX-BSD-tet-mEGFP-MIB1

    Plasmid

    #140241

    Purpose

    Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    Tet-inducible mEGFP-MIB1 (MIB1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

    Plasmid

    #204146

    Purpose

    Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Inserts

    SARS-CoV-2 BA.1 variant spike
    zsGreen-T2A-PuR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV and TRE3G

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #168997

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. AAV.CBA.eGFP.2A.wtTau

    Plasmid

    #140424

    Purpose

    AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau (MAPT Human, Synthetic)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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