We narrowed to 13,963 results for: eng
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Plasmid#141184PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFPDepositorHas ServiceCloning Grade DNAInsertS surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
TagsSignal peptide from influenza HA and Superfolder …ExpressionMammalianPromoterCMVAvailable SinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-EK_C122S_His5
Plasmid#49048PurposeExpresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coliDepositorInsertEnteropeptidase, peptidase domain (TMPRSS15 Bovine)
Tags5xHisExpressionBacterialMutationP92R, E185D, C122SPromoterT7Available SinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Blast
Plasmid#85133Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralPromoterEF1aAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC-Kan-alphaGal4
Plasmid#13421DepositorInsertRNAP alpha-Gal4
ExpressionBacterialAvailable SinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Hygro
Plasmid#85134Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralPromoterEF1aAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)
Plasmid#24859DepositorAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-opti
Plasmid#106280PurposeA version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
gRNA_GFP-T1
Plasmid#41819PurposeExpresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T1
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pdCas9
Plasmid#46569PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.DepositorInsertstracrRNA
dcas9
CRISPR array
UseCRISPRExpressionBacterialMutationD10A, H840AAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
800 pSG5L HA PTEN wt
Plasmid#10750DepositorAvailable SinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
OCT4-2A-eGFP-PGK-Puro
Plasmid#31938PurposeHuman OCT4 genomic targeting donorDepositorAvailable SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42337PurposeDual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA blast
Plasmid#104993PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHR_pGK_LaG17_synNotch_Gal4VP64
Plasmid#79127PurposeLentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)DepositorInsertLaG17_synNotch_Gal4DBDVP64
UseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T1
Plasmid#41817PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T1
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003
Plasmid#133447PurposeExpresses SpyCatcher003 in bacterial cytoplasmDepositorInsertSpyCatcher003
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXAT2
Plasmid#80494PurposeAAVS1 sgRNA expression vectorDepositorInsertAAVS1 sgRNA-T2
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro
Plasmid#31482DepositorInsertTraffic Light Reporter 1.1 (Sce Target) EF Puro
UseLentiviralMutationmCherry has M9S and M16L mutations. This is what …Available SinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only