4,124 results

1. mCerulean N1

   Plasmid

   #27795

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Insert

   mCerulean N1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SIRT6 Flag

   Plasmid

   #13817

   Purpose

   Mammalian expression of human SIRT6 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-mGFP-Actin

   Plasmid

   #21948

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

   Insert

   betaActin (ACTB Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpCas9n(BB)-2A-Puro (PX462)

   Plasmid

   #48141

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pBABE-FLAG-KD LKB1

   Plasmid

   #8593

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   LKB1 (STK11 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K78I: lysine 78 mutated to isoleucine; kinase dead

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pk7-GR-GFP

   Plasmid

   #15534

   Depositor

   Ian Macara

   Article

   Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

   Insert

   Glucocorticoid receptor (NR3C1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The glucocorticoid receptor was cloned into the X…

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-Raichu-RhoA-CR

   Plasmid

   #40258

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Raichu-RhoA-CR (RHOA Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLifeAct_mScarlet-i_N1

   Plasmid

   #85056

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   LifeAct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pClneoMyc-LATS2

   Plasmid

   #66852

   Purpose

   Express Myc-tagged LATS2 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

   Insert

   LATS2 (LATS2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-Flag-hMcl-1

    Plasmid

    #25392

    Depositor

    Roger Davis

    Insert

    MCL1 (MCL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDH-puro-myr-HA-Akt1

    Plasmid

    #46969

    Purpose

    lentiviral expression of myr-HA-Akt1

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-HDAC4

    Plasmid

    #45636

    Depositor

    Tso-Pang Yao

    Article

    Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

    Insert

    HDAC4 (HDAC4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSBtet-RP

    Plasmid

    #60497

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVSV Venus VSV-G

    Plasmid

    #36399

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    VSV Genome
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    VSV promoters

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pINDUCER11 (miR-RUG)

    Plasmid

    #44363

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hCathepsin L

    Plasmid

    #11250

    Purpose

    Mammalian expression of human cathepsin L

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Has Service

    Cloning Grade DNA

    Insert

    cathepsin L (CTSL Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pL-CRISPR.SFFV.GFP

    Plasmid

    #57827

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag-HA-USP15

    Plasmid

    #22570

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP15 (USP15 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-HIF1 alpha

    Plasmid

    #21101

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTAT-HA

    Plasmid

    #35612

    Depositor

    Steven Dowdy

    Article

    Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

    Type

    Empty backbone

    Use

    Tags

    6xHIS, HA, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mEGFP

    Plasmid

    #18696

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    monomeric EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hAur-A

    Plasmid

    #8510

    Depositor

    Joan Ruderman

    Article

    Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

    Insert

    hAur-A (AURKA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMK286 (mAID-NeoR)

    Plasmid

    #72824

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Neo
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSL-MS2-6X

    Plasmid

    #27118

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    MS2 binding sites (6)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Flag-TRIM24

    Plasmid

    #28138

    Depositor

    Michelle Barton

    Article

    Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pU6-sgGFP-NT1

    Plasmid

    #46914

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgGFP-NT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. cEF.tk-GFP

    Plasmid

    #33308

    Depositor

    Linzhao Cheng

    Article

    Pomper et al Cell Res. 2009 Mar;19(3):370-9.

    Insert

    HSV-TK/GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Tet-O-FUW-Ascl1

    Plasmid

    #27150

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV-N-Flag-HA-IRES-PURO

    Plasmid

    #41033

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Type

    Empty backbone

    Use

    Retroviral; Gateway destination vector

    Tags

    Flag, HA, and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    LTR-driven expression

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EBFP2-C1

    Plasmid

    #54665

    Purpose

    Localization: C1 Cloning Vector, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge