4,129 results

1. xCas9 3.7

   Plasmid

   #108379

   Purpose

   Mammalian expression vector for xCas9 3.7

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.7
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lentiviral-FOP-dGFP-reporter

   Plasmid

   #14885

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   mutant LEF-1/TCF-responsive promoter (Ctnnb1 )
   

   Use

   Lentiviral

   Tags

   d2 eGFP

   Expression

   Mammalian

   Mutation

   mutant TCF/LEF binding sites

   Promoter

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe 12S E1A

   Plasmid

   #18742

   Depositor

   Scott Lowe

   Article

   Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

   Insert

   12S E1A
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-ReaChR-citrine

   Plasmid

   #50954

   Purpose

   ReaChR-citrine in AAV2 vector under human synapsin promoter

   Depositor

   Roger Tsien

   Article

   Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

   Insert

   ReaChR-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   Promoter

   truncated human Synapsin promoter

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-sgGFP-NT1

   Plasmid

   #46914

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   sgGFP-NT1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-UtrCH

   Plasmid

   #26737

   Depositor

   William Bement

   Article

   Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

   Insert

   Utrophin
   

   Use

   Reporter for f-actin

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Calponin homology domain of utrophin (aa# 1-261).…

   Promoter

   CMV IE94

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. MLM3613

   Plasmid

   #42251

   Purpose

   Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   Streptococcus pyogenes Cas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTAT-HA

   Plasmid

   #35612

   Depositor

   Steven Dowdy

   Article

   Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

   Type

   Empty backbone

   Use

   Tags

   6xHIS, HA, and TAT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCas9-T2A-BFP

   Plasmid

   #78547

   Purpose

   Cas9 protein with BFP

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   In frame with Cas9

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRFP-Clc

    Plasmid

    #14435

    Depositor

    Ari Helenius

    Article

    Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

    Insert

    Clathrin light chain (Clc Mouse)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

    Plasmid

    #61762

    Purpose

    Dual Color betaGlobin reporter to study splicing kinetics in vivo

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    b-Globin
    

    Use

    Tags

    24 X MS2 hairpin cassette in 3'UTR of b-Glob…

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible Tet-ON promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR4-V5 (w71-3)

    Plasmid

    #17425

    Purpose

    Gateway entry vector for N-term V5 tag, frame A

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. p212 pCMV-EGFP/RFP

    Plasmid

    #8389

    Purpose

    Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    floxed EGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-Wnt5A

    Plasmid

    #35911

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt5A (WNT5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE EF1α dCas9-KRAB

    Plasmid

    #50919

    Purpose

    Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMVTNT PINK1 N-myc

    Plasmid

    #13313

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ac5-STABLE2-neo

    Plasmid

    #32426

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Insert

    Flag-mCherry-T2A-GFP-T2A-neo
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. phage-cmv-cfp-24xms2

    Plasmid

    #40651

    Depositor

    Robert Singer

    Article

    Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

    Insert

    CFP-24xMBS
    

    Use

    Lentiviral

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG HRP-TM

    Plasmid

    #44441

    Depositor

    Alice Ting

    Article

    Rhee et al Science. 2013 Jan 31.

    Insert

    Horseradish peroxidase fused to the transmembrane domain of PDGFR
    

    Use

    Tags

    HA tag and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. p40PX-EGFP

    Plasmid

    #19010

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p40phox (NCF4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-148 (PX domains)

    Promoter

    CMV

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. pC1-REX-YFP

    Plasmid

    #48247

    Purpose

    Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    REX-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MSCV h c-MYC IRES GFP

    Plasmid

    #18119

    Depositor

    John Cleveland

    Insert

    c-MYC (MYC Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    The CUG which is the start site for Myc I is pres…

    Promoter

    Available since

    June 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mTagBFP2-TOMM20-N-10

    Plasmid

    #55328

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

    Plasmid

    #44028

    Depositor

    Celeste Simon

    Article

    Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

    Insert

    mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P402A, P577A, N813A

    Promoter

    CMV

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-Flpe:GFP

    Plasmid

    #13788

    Purpose

    Mammalian expression of enhanced form of Flp recombinase fused to GFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Flpe-GFP fusion protein
    

    Use

    Flp/frt

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. G6PD/pRK5

    Plasmid

    #41521

    Depositor

    Xiaolu Yang

    Article

    Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

    Insert

    glucose-6-phosphate dehydrogenase (G6PD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQFlag-USP7 WT puroR

    Plasmid

    #46751

    Purpose

    Retroviral vector that expresses wild type Flag-tagged human USP7

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. hAur-A

    Plasmid

    #8510

    Depositor

    Joan Ruderman

    Article

    Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

    Insert

    hAur-A (AURKA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

    Plasmid

    #31481

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce target) EF BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry contains M9S and M16L to reduce backgroun…

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. -962 human cyclin D1 promoter pGL3Basic

    Plasmid

    #32727

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase; Luciferase reporter gene

    Tags

    Expression

    Mutation

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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