166,201 results

1. pAS_FLAG-Mx1201 PylRS

   Plasmid

   #154764

   Purpose

   plasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas9 sgRNA vector

   Plasmid

   #68463

   Purpose

   Cas9 sgRNA vector for cloning

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pepper-fused sgRNA

   Plasmid

   #217520

   Purpose

   Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

   Depositor

   Xing Li

   Article

   Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

   Insert

   Pepper-fused sgRNA for spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

   Plasmid

   #122964

   Purpose

   Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.

   Depositor

   FENG-QIAN Li, Ken-Ichi Takemaru

   Insert

   human Chibby1 (CBY1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Promoter

   FoxJ1 and human FoxJ1

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCast

   Plasmid

   #200169

   Purpose

   E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

   Depositor

   Yong-Su Jin

   Article

   Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-BLK

   Plasmid

   #23940

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   BLK (BLK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

   Plasmid

   #171615

   Purpose

   Soma-targeted Cal-Light with KA2

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
   

   Use

   Synthetic Biology

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-HLA-A0201-His

   Plasmid

   #108213

   Purpose

   HLA-A0201 his tagged

   Depositor

   Sébastien Walchli

   Article

   Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

   Insert

   major histocompatibility complex, class I, B (HLA-A Human)
   

   Use

   Entry vector for gateway system

   Tags

   his

   Available Since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq)

   Plasmid

   #230015

   Purpose

   Expresses anti-TNFalpha PAGER(Gq) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-Ozoralizumab-TEVcs-ALFA-hM1D
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   W442A on hM1D

   Promoter

   CMV

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225703

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K36M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    K36M

    Promoter

    pEF1A

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSW002-Pc-DsRed-Express2

    Plasmid

    #111251

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    DsRed-Express2
    

    Expression

    Bacterial

    Promoter

    Pc

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. EIF2AK4 gRNA (BRDN0001146469)

    Plasmid

    #75876

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. C1-FT-mCitrine-EEVEE-CaaX

    Plasmid

    #155225

    Purpose

    MPAct with increased linker flexiblity

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPIDNB

    Plasmid

    #169901

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Aivian expression

    Expression

    Mammalian

    Promoter

    Dox inducible

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ14901

    Plasmid

    #239276

    Purpose

    Expresses gG1 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PspCas13b-hGAPDH-gRNA1 (GAPDH Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1A-CD19.8H.8TM.BB.3z

    Plasmid

    #200671

    Purpose

    Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSW002

    Plasmid

    #111248

    Purpose

    Broad host-range bacterial vector

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBNP-NFAT-luciferase

    Plasmid

    #24956

    Depositor

    Toren Finkel

    Insert

    BNP promoter (NPPB Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF-N-HiBiT-template

    Plasmid

    #200882

    Purpose

    template for N-terminal HiBIT-tagging

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    HiBIT-linker
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK344 (Hygro-P2A-mAID)

    Plasmid

    #121178

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. EGFP-alphasynuclein-A53T

    Plasmid

    #40823

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A53T (aggregates more rapidly than the wild-type …

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. MSCV-JAK3(M511)_IRES_GFP

    Plasmid

    #206290

    Purpose

    Expression of mutant JAK3 (M511I) that is able to drive constitutive activation of the JAK/STAT pathway

    Depositor

    Charles de Bock

    Article

    Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

    Insert

    JAK3 (M511I)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Methionine 511 to Isoleucine

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAS_FLAG-G1 PylRS

    Plasmid

    #154763

    Purpose

    plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RAB27B

    Plasmid

    #25545

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB27B (RAB27B Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

25. FLYLQ

    Plasmid

    #80421

    Purpose

    expresses cDNA for human CELF1 (CUGBP1) Flag tagged

    Depositor

    Thomas Cooper

    Article

    Kalsotra et al Proc Natl Acad Sci U S A. 2008 Dec 23;10

    Insert

    human CELF1 cDNA (CELF1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. dCas9-SunTag(10X)-2A-BFP

    Plasmid

    #227389

    Purpose

    Adhesion module, dCas9-SunTag

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    dCas9-SunTag
    

    Use

    Lentiviral

    Mutation

    Enzymatically dead Cas9

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pB80-hsKIF5B(1-560)Rigor-L-2xHA

    Plasmid

    #193718

    Purpose

    Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-2xHA (KIF5B Human, Synthetic)
    

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKLV-EF1a-BFP-W

    Plasmid

    #208757

    Purpose

    Lentiviral vector expressing BFP

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEM705 Gs15

    Plasmid

    #109360

    Purpose

    GsX chimera with Gα15 C-terminus

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gs15 chimera
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mEGFP-N1-MST2

    Plasmid

    #166475

    Purpose

    Expresses fusion of mEGFP and MST2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, MST2 (STK3 Human)
    

    Expression

    Mammalian

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEasyG1_nat

    Plasmid

    #184911

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pB80-2xFKBP-L-mCherry-CAAX

    Plasmid

    #193713

    Purpose

    Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    2xFKBP-L-mCherry-CAAX
    

    Tags

    2xFKBP-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1 Deleted

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EIF2AK1 gRNA (BRDN0001162524)

    Plasmid

    #77050

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK1 (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCIN BRCA2 WT

    Plasmid

    #16245

    Depositor

    Mien-Chie Hung

    Article

    Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

    Insert

    BRCA2 (BRCA2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHAGE-EPHA3

    Plasmid

    #116733

    Purpose

    Lentiviral expression of EPHA3

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pmr101A-cPr-mCherry (ErmR)

    Plasmid

    #177206

    Purpose

    Plasmid with p15A origin for constitutive mCherry expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    Synthetic constitutive promoter

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEF-hSEMA4C-Fc

    Plasmid

    #74449

    Purpose

    Expresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cells

    Depositor

    Roland Friedel

    Article

    Le et al Oncotarget. 2015 Mar 30;6(9):7293-304.

    Insert

    human Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)
    

    Tags

    human Fc tag

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLKO.1 - TRC control

    Plasmid

    #10879

    Purpose

    Negative control shRNA vector containing non-hairpin insert.

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Insert

    non-hairpin 18bp
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pmPB3-VSV

    Plasmid

    #68039

    Purpose

    Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B3 (Plxnb3 Mouse)
    

    Tags

    VSV

    Expression

    Mammalian

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge