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163,682 results

Showing: 3841 - 3880 of 163682 results

  1. TFORF2258

    Plasmid
    #142927
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX11 (SOX11 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142927-plasmid-map-sequence-id-397307

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  3. pAC-LYC

    Plasmid
    #53270
    Purpose
    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53270-plasmid-map-sequence-id-487542

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  5. CLYBL-TO-hNIL-BSD-mApple

    Plasmid
    #124230
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Insert
    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124230-plasmid-map-sequence-id-240604

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  8. ADCYAP1R1-Tango

    Plasmid
    #66208
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADCYAP1R1 (ADCYAP1R1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pINDUCER20-hMYOD1-3xHA (#730)

    Plasmid
    #78328
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYOD1 (MYOD1 Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Expression
    Mutation
    Promoter
    TRE2
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78328-plasmid-map-sequence-id-424966

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  12. pMAH-POLY-eRF1(E55D)

    Plasmid
    #157925
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-
    Insert
    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    157925-plasmid-map-sequence-id-310653

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  13. pPlatTET-gRNA2

    Plasmid
    #82559
    Purpose
    All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.
    Depositor
    Izuho Hatada
    Article
    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103
    Insert
    dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    82559-plasmid-map-sequence-id-207901

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  23. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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  24. pLEX307-TMPRSS2-G418

    Plasmid
    #158459
    Purpose
    Constitutive lentiviral expression of human TMPRSS2.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    158459-plasmid-map-sequence-id-311225

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  28. pPbcas13b

    Plasmid
    #89906
    Purpose
    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available Since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89906-plasmid-map-sequence-id-178224

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  30. TFORF2957

    Plasmid
    #144433
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF4A (HNF4A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144433-plasmid-map-sequence-id-407322

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  31. TFORF2003

    Plasmid
    #142902
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXD3 (FOXD3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142902-plasmid-map-sequence-id-397561

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  35. pE-SUMO CRYAA

    Plasmid
    #80756
    Purpose
    Expression of human alpha A-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYAA (CRYAA Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80756-plasmid-map-sequence-id-153571

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Showing: 3841 - 3880 of 163682 results