164,231 results

1. psPAX2

   Plasmid

   #12260

   Purpose

   2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Lentiviral; Packaging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMD2.G

   Plasmid

   #12259

   Purpose

   VSV-G envelope expressing plasmid

   Depositor

   Didier Trono

   Insert

   VSV G
   

   Use

   Lentiviral; Envelope

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV(CAT)T7-SB100

   Plasmid

   #34879

   Depositor

   Zsuzsanna Izsvak

   Article

   Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

   Insert

   SB100X transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-PEmax-P2A-GFP

   Plasmid

   #180020

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-EGFP
   

   Use

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pV238-vPE

   Plasmid

   #225258

   Purpose

   Mammalian expression of vPE

   Depositor

   Robert Langer, Phil Sharp, Vikash Chauhan

   Article

   Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

   Insert

   vPE
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   R221K K848A H982A N1317R

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL3-NFAT luciferase

   Plasmid

   #17870

   Purpose

   3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

   Depositor

   Jerry Crabtree

   Article

   Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

   Insert

   3x NFAT binding sequence (IL2 )
   

   Use

   Luciferase

   Tags

   IL2 promoter and Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   IL-2 promoter

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SP-dCas9-VPR

   Plasmid

   #63798

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDH-EF1a-mCherry-EGFP-LC3B

    Plasmid

    #170446

    Purpose

    Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    mCherry-GFP-LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pROSA26-PA

    Plasmid

    #21271

    Depositor

    Frank Costantini

    Article

    Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sortase A pentamutant (eSrtA) in pET29

    Plasmid

    #75144

    Purpose

    evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

    Depositor

    David Liu

    Article

    Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

    Insert

    sortase A pentamutant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T. Deleted amino a…

    Promoter

    T7

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pA-His6-Tn5

    Plasmid

    #171871

    Purpose

    Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Protein A and hyperactive Tn5 transposase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 3XFlag-pA-Tn5-Fl

    Plasmid

    #124601

    Purpose

    Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

    Depositor

    Steven Henikoff

    Article

    Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

    Insert

    Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
    

    Use

    Tags

    Protein A and Tn5 transposase fusion protein with…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLCAR-CD19-BBz

    Plasmid

    #135992

    Purpose

    Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Promoter

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MaLionR

    Plasmid

    #113908

    Purpose

    Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 8xGTIIC-luciferase

    Plasmid

    #34615

    Purpose

    YAP/TAZ-responsive synthetic promoter driving luciferase expression

    Depositor

    Stefano Piccolo

    Article

    Dupont et al Nature. 2011 Jun 8;474(7350):179-83. doi

    Insert

    synthetic TEAD luciferase reporter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLCAR-CD19-28z

    Plasmid

    #135991

    Purpose

    Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available Since

    June 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge