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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,942 results for: LEA

Showing: 5001 - 5050 of 15942 results

  4. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  7. pET21-pelB-LaG-6

    Plasmid
    #172741
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-6
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172741-plasmid-map-sequence-id-340175

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  8. CMV/N3IC-HA

    Plasmid
    #47618
    Purpose
    in vitro transcription and translation of HA-tagged Notch3 Intracellular domain
    Depositor
    Urban Lendahl
    Article
    Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.
    Insert
    Notch3 Intracellular domain (Notch3 Mouse)
    Use
    In vitro transcription and translation
    Tags
    HA
    Expression
    Mammalian
    Mutation
    codons 1664 (Met) to 2318 (Ala)
    Promoter
    CMV
    Available Since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pBAD24-rnc-sfGFP

    Plasmid
    #51560
    Purpose
    Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).
    Depositor
    Francisco Malagon, Sankar Adhya
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    rnc (rnc E. coli)
    Use
    Tags
    GLESTCRHASLAVLADERRFSA and superfolder GFP
    Expression
    Bacterial
    Mutation
    Contains additonal aa at the end of the sfGFP in …
    Promoter
    Available Since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51560-plasmid-map-sequence-id-78838

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  27. pTRIPZ-Myr-PTEN

    Plasmid
    #206983
    Purpose
    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal
    Depositor
    Ming Chen
    Article
    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:
    Insert
    PTEN (PTEN Human)
    Use
    Lentiviral
    Tags
    N-terminal myristoylation site
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on promoter
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206983-plasmid-map-sequence-id-408428

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  29. pOCC292

    Plasmid
    #118881
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    mRuby3, HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118881-plasmid-map-sequence-id-232308

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  37. pOCC291

    Plasmid
    #118867
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mRuby3
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118867-plasmid-map-sequence-id-232275

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  39. pTSSX-STC

    Plasmid
    #115668
    Purpose
    E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag
    Depositor
    David Kramer
    Article
    Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459
    Insert
    small tetraheme cytochrome c
    Use
    Tags
    Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available Since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    115668-plasmid-map-sequence-id-228494

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  46. cycLuc_PPVs

    Plasmid
    #119208
    Purpose
    Circularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with PPVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mutation
    Promoter
    CMV
    Available Since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    119208-plasmid-map-sequence-id-244622

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  48. pOCC61

    Plasmid
    #118877
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    mGFP, HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118877-plasmid-map-sequence-id-232346

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Showing: 5001 - 5050 of 15942 results