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Plasmid#98942PurposeMammalian expression plasmid for untagged human CXCR4DepositorInsertCXCR4 (CXCR4 Human)
UseTagsExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK2-R67S/S153D
Plasmid#213597PurposeDoxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cellsDepositorInsertMAPK1 (MAPK1 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR67S, S153DPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT_025
Plasmid#158579PurposeLentiviral expression of a customizable open reading frameDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChR2(H134R)-eYFP (AAV PHP.eB)
Viral Prep#127090-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-CAG-DIO-ChR2(H134R)-eYFP (#127090). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-ChR2(H134R)-eYFP plasmid DNA. CAG-driven, Cre-dependent expression of humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEYFP (Cre-dependent)Available sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBB542
Plasmid#27395DepositorInsertdnaK, dnaJ, groESL
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBsacA
Plasmid#158650PurposeConstitutive transcription of sacA crRNADepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…TagsExpressionBacterialMutationPromoterPvegAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.EF1a.eGFP.WPRE.rBG
Plasmid#105547PurposeAAV expression of EGFP from EF1a promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV8InsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-Cdc42-C1
Plasmid#29673DepositorInsertCdc42 (CDC42 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
Frb-NLS-mCherry
Plasmid#217613Purposepart B of SPARK-OFFDepositorInsertFrb-NLS-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA14 (MSW#503)
Plasmid#208069PurposeExpression of GFP-tagged hsRPA3 in human cellsDepositorInsertRPA3 (RPA3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mTurquoise2
Plasmid#98828PurposeIn vivo visualization of lysosomes (can be used for colocalization studies)DepositorArticleInsertLamp1
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mito-TagRFP-T
Plasmid#174543Purposelabels mitochondria with TagRFP-TDepositorInsertMTS-tagRFP-T
UseLentiviralTagstagRFP-TExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2E2-HA-mEGFP
Plasmid#129595PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV rtTA3 Hygro (w785-1)
Plasmid#26730Purpose3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, HygroDepositorInsertTetracycline repressor A3 mutant
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_GCAMP7s
Plasmid#182940Purposealpha7 nACHR expression in mammalian cell lines with calcium reporterDepositorInsertNACH7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET14b – E. coli Exonuclease I
Plasmid#104552Purposebacterial expression of Exonuclease IDepositorInsertExonuclease I (sbcB E.coli)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W
Plasmid#67977PurposeCRISPR gRNA expression vector with an improved scaffold and puro/mCherry markersDepositorInsertU6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6d
Plasmid#215822PurposeExpress CBE6d (with SpCas9) in mammalian cellsDepositorInsertCBE6d-SpCas9-2xUGI (cas9 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GRM5-DuET
Plasmid#213303PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGRM5 (GRM5 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM4D(Gi)
Plasmid#45548PurposeExpresses hM4D (Gi) neuronal inhibitorDepositorInserthM4D (Gi)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCRISPRi-v2, Membrane Proteins (m6), top 5 sgRNAs/gene
Pooled Library#83994PurposeMouse CRISPRi Pooled Library targeting membrane proteinsDepositorAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-mMLKL
Plasmid#208784PurposeInducibly expresses mouse MLKL fused to light-activatable Cry2DepositorInsertmCherry mMLKL Cry2 (Mlkl Mouse)
UseLentiviralTagsCry2 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CW3SL-EGFP
Plasmid#61463PurposeAAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)DepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
EZ-Tet-pLKO-Blast
Plasmid#85973PurposeTet/Dox inducible shRNA lentivirus with blasticidin selectionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterH1 / TetOAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP
Plasmid#37825DepositorHas ServiceAAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1InsertGFP
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationN/APromoterCAGAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-LivePAR-Hygro
Plasmid#176063PurposeEGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassetteDepositorInsertLivePAR
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-Arl13b12
Plasmid#40872DepositorInsertArl13b (Arl13b Mouse)
UseTagsGFPExpressionMammalianMutationno stop codonPromoterCMVAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP-TurboID-NES_pLX304
Plasmid#202019Purposeexpresses GFP-TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-GFP-TurboID-NES
UseLentiviralTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA hygro
Plasmid#104991PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Phusion
Plasmid#206210PurposeInducible expression of DNA polymeraseDepositorInsertPhusion DNA polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-scFvGCN4sfGFPTET1CD
Plasmid#82561PurposeExpression vector for antibody-sfGFP-TET1CD.DepositorInsertscFvGCN4sfGFPTET1CD
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Ef1a-mCherry (AAV8)
Viral Prep#114470-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherryAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-eYFP (AAV2)
Viral Prep#117382-AAV2PurposeReady-to-use AAV2 particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-P4Mx1
Plasmid#108143PurposePI4P biosensorDepositorInsertX.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZP06C
Plasmid#232322PurposepZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterrpsJAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
C1QBP-bio-His
Plasmid#53330PurposeExpresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertC1QBP (C1QBP Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-4EBP1_4xAla
Plasmid#38240DepositorInsert4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationT37A, T46A, S65A, T70A (dominant negative)PromoterTet ONAvailable sinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -