We narrowed to 11,202 results for: ena
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Plasmid#67629PurposeMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOraiDepositorInsertdmBACCS2VL-IRES-dOrai
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
ND4 right TALE–G1333 DddAtox N
Plasmid#158096Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg1+sg2+sg3
Plasmid#113969PurposeTriple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTCDepositorInsertsg1+sg2+sg3
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (OTU, aa 287-437)
Plasmid#61407PurposeExpresses human OTUD1 (OTU domain) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286 and aa 438-481.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD
Plasmid#168135PurposeInducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.DepositorInsertsAvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_AB
Plasmid#66004PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNRDc-E>A-V5
Plasmid#51298Purposeexpression of the inactive mutant of mouse NRDc in mammalian cellsDepositorInsertNRDc E247A (Nrd1 Mouse)
UseTagsHIS and V5ExpressionMammalianMutationE247A, enzymatic inactive mutantPromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_26G
Plasmid#91149PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:barDepositorInsertEngineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
G1333 DddAtox-C–SaKKH-Cas9(D10A)
Plasmid#157838Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RIPK1 K115R
Plasmid#78835PurposeTo overexpress RIPK1 K115R in Mammalian CellsDepositorInsertRIPK1 (RIPK1 Human)
UseTagsHAExpressionMammalianMutationK115RPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPPC001
Plasmid#171138PurposeFor integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-MCS-P2A-iLIDcaax
Plasmid#173864PurposeMammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)DepositorInsertmVenus-NES-SspB-MCS-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUB2 (full-length, aa 1-234)
Plasmid#61421PurposeExpresses human OTUB2 (full-length) in E. coli.DepositorInsertOTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos4b (mEosFP4b-V69T)
Plasmid#99226PurposeBacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).DepositorInsertpr-mEos4b
UseTagsExpressionBacterialMutationV69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
G1397 DddAtox-N–SaKKH-Cas9(D10A)
Plasmid#157839Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25G
Plasmid#91144PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf117
Plasmid#12805PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf117
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPKm-196
Plasmid#90511PurposepcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoterDepositorInsertPIF6-DBD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G2-RacE
Plasmid#188966PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: gttagacgctgattacatggactagg
racE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf42
Plasmid#12730PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf42
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS1-mEGFP-NLS
Plasmid#205013PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mCherry-NLS
Plasmid#205009PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.DepositorInsertmCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha1 clone2
Plasmid#162120PurposeLentiviral sgRNA plasmid targeting human AMPK alpha1DepositorInsertsgAMPK alpha1 (PRKAA1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEXNKI-GST-3C-LIC
Plasmid#108710PurposeExpression of a GST-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsGST-3C protease cleavage siteExpressionBacterialMutationPromotertacAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV-MS2-TV
Plasmid#136381PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion proteinDepositorInsertAaCas12b (D570A)-TV-T2A-MCP-TV
UseCRISPRTagsExpressionPlantMutationD570APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_23D
Plasmid#91141PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS6: pHelper(AvCAST)_entry_ΔTnsD
Plasmid#168139PurposeInducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.DepositorInsertsAvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_jRCaMP1b
Plasmid#183758PurposeBxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporterDepositorInsertjRCaMP1b
UseTagsNESExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf196
Plasmid#12884PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf196
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 PE2-3HA
Plasmid#169853PurposeMammalian Expression, SpyCas9 prime editor with 3HA-tagDepositorInsertSpCas9-H840A-M-MLV-3HA
UseTags3XHA tag and bpSV40 NLSExpressionMammalianMutationPromotercmvAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-kan
Plasmid#108706PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
BRD4 CRISPRi plasmid
Plasmid#154890PurposeHuman BRD4 CRISPRi gRNADepositorInsertBRD4-targeting gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
E1010m (RFP)_CD
Plasmid#66033PurposeMoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]DepositorInsertFluorescent reporter - RFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0-iRFP670-micro
Plasmid#135954PurposeExpresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells.DepositorInsertiLID micro (SspB R73Q)
UseLentiviralTagsiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-sCAG-GluClβ.CreON
Plasmid#196996PurposeCre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.DepositorInsertGluClβ -YFP
UseAAV and Cre/LoxTagsYFPExpressionMutationPromotershort CAGAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_CDK8
Plasmid#111685PurposeMAC-tagged gene expressionDepositorInsertCDK8 (CDK8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W
Plasmid#105025PurposeLentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFPDepositorInsertNelfcd (Th1l Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W
Plasmid#105026PurposeLentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFPDepositorInsertNelfcd (Th1l Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25I
Plasmid#91146PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_GSK3B
Plasmid#111687PurposeMAC-tagged gene expressionDepositorInsertGSK3B (GSK3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only